First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan

Kouda, Shuntaro; Kuwahara, Ryuichi; Ohara, Masaru; Shigeta, Masanobu; Fujiwara, Takanori; Komatsuzawa, Hitoshi; Usui, Tsuguru; Sugai, Motoyuki

doi:10.1007/s10156-007-0520-0

Cited by 15 publications

(9 citation statements)

References 8 publications

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“…Carbapenems have been used to treat difficult gram-negative infections due to their broad spectrum of activity and resistance to hydrolysis by most β-lactamases. However, prevalence of carbapenem-resistant P. aeruginosa has increased worldwide (1,2). Carbapenem resistance in P. aeruginosa may be mediated by decreased outer membrane permeability, upregulation of multidrug efflux pumps, interplay between impermeability, and production of β-lactamases or carbapenemases such as metallo-βlactamases (MBLs) (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

“…MBLs hydrolyze all β-lactam antibiotics with the exception of aztreonam, are inhibited by metal chelators such as ethylenediaminetetraacetic acid (EDTA) and thiol compounds, and are resistant to serine β-lactamase inhibitors such as clavulanate and tazobactam. MBLencoding genes are divided into 6 groups: IMP, VIM, SIM, SPM, GIM, and AIM (2,7,8). These genes are usually integron-mediated and can be carried by transferable plasmids, or may be chromosomal (8).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of blaIMP and blaVIM gene carriage in metallo-β–lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran

Salimi¹,

Eftekhar²

2014

Turk J Med Sci

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Background/aim: To study the prevalence of bla VIM and bla IMP genes in metallo-β-lactamase (MBL)-producing burn isolates of Pseudomonas aeruginosa in relation with AmpC and extended-spectrum β-lactamase (ESBL) production. Materials and methods:Thirty-two carbapenem-resistant MBL-producing P. aeruginosa burn isolates from Shahid Motahari Burn Hospital in Tehran were employed. Antibiotic susceptibility was determined to 13 antibiotics including imipenem and meropenem by disk diffusion. AmpC and ESBL production was detected by the AmpC disk test and combined disk diffusion assay, respectively. bla IMP and bla VIM gene carriage was shown by polymerase chain reaction and type-specific primers.Results: AmpC production was observed in 81% and ESBL production was detected in 12.5% of the isolates. bla IMP carriage was observed in 56.25% and bla VIM gene in 46.8% of the isolates. Surprisingly, 43.5% of the isolates carried both bla IMP and bla VIM genes. Conclusion:We think that this is the first report on the cocarriage of bla IMP and bla VIM in P. aeruginosa. There was also a strong association between MBL gene carriage and AmpC β-lactamase production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence of blaIMP and blaVIM gene carriage in metallo-β–lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran

Salimi¹,

Eftekhar²

2014

Turk J Med Sci

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“…Although prevalent in Asia, in Europe IMP-producing P. aeruginosa has been observed mostly in Italy so far (17). IMP-7 has been reported in Canada (6), Malaysia (7), and Japan (10,20), but to our knowledge no MLST of the producer P. aeruginosa strains has been performed. Integronic arrays with bla IMP-7 in the Canadian and Japanese isolates were different than those here (6,20).…”

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confidence: 95%

Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-β-Lactamase in Central Europe

et al. 2011

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“…MBL‐producing ST235 isolates seem to be the most widely spread clones associated with multidrug resistance and are frequently detected in several countries . P. aeruginosa ST235 and ST357 isolates with IMP‐1, IMP‐7 or VIM‐1 have been identified in several countries, including Central Europe . ST235 isolates with IMP‐1 and IMP‐7, and ST357 isolates with IMP‐1 have already been reported, but ST235 isolates with VIM‐1 or ST357 isolates with IMP‐7 have not been reported in Japan .…”

Section: Discussionmentioning

confidence: 99%

“…4,5,28,29 ST235 isolates with IMP-1 and IMP-7, and ST357 isolates with IMP-1 have already been reported, but ST235 isolates with VIM-1 or ST357 isolates with IMP-7 have not been reported in Japan. 2,5,28,29 Therefore, we newly identified the ST235 isolate with VIM-1 in Japan. Furthermore, in ST235 isolates, the isolates with IMP-1 (rep-PCR type a) or the isolates with VIM-1 or IMP-7 (rep-PCR type b) were closely related, as shown by rep-PCR, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of carbapenem‐resistant Pseudomonas aeruginosa isolated from urine in Hyogo, Japan

et al. 2018

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Objectives To investigate the molecular characteristics and epidemiology of metallo‐β‐lactamase‐producing Pseudomonas aeruginosa from urine of urinary tract infection patients in Hyogo Prefecture, Japan. Methods Carbapenem‐resistant P. aeruginosa isolated from the urine of 21 urinary tract infection patients in three general hospitals in Hyogo Prefecture (Japan) were collected between 2007 and 2014. Their antibiotic susceptibilities, metallo‐β‐lactamase screening test, metallo‐β‐lactamase gene sequencing, multilocus sequence typing and repetitive‐sequence‐based polymerase chain reaction were determined for epidemiological analyses to investigate the genetic characteristics. Results Out of 21 isolates, 13 (61.9%) were positive for metallo‐β‐lactamase. There were 11 (52.4%) isolates with IMP‐1 in them, one (4.5%) isolate with IMP‐7 and one (4.5%) isolate with VIM‐1. Metallo‐β‐lactamase‐positive isolates were mainly identified as ST235, and metallo‐β‐lactamase‐negative isolates were STs 357, 277, 234, 439 and 639. Repetitive‐sequence‐based polymerase chain reaction showed metallo‐β‐lactamase‐positive isolates were grouped in eight clusters, and ST235 isolates with IMP‐1 from three hospitals belonging to the identical group I, the other ST235 isolates with IMP‐7 and VIM‐1 were from two hospitals belonging to group II. Conclusions Metallo‐β‐lactamase‐positive P. aeruginosa of ST235 isolates with IPM‐1 were mainly identified from the urine of urinary tract infection patients in Hyogo, Japan. A ST235 isolate with VIM‐1 was found for the first time. Further investigation is necessary to follow the spread of metallo‐β‐lactamase‐positive isolates.

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First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan

Cited by 15 publications

References 8 publications

Prevalence of blaIMP and blaVIM gene carriage in metallo-β–lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran

Prevalence of blaIMP and blaVIM gene carriage in metallo-β–lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran

Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-β-Lactamase in Central Europe

Molecular characteristics of carbapenem‐resistant Pseudomonas aeruginosa isolated from urine in Hyogo, Japan

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