First isolation of Coxiella burnetii from clinical material by cell-free medium (ACCM2)

Boden, Katharina; Wolf, Katharina; Hermann, Beate; Frangoulidis, Dimitrios

doi:10.1007/s10096-015-2321-1

Cited by 23 publications

(14 citation statements)

References 22 publications

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“…They proposed using two panels of markers to have a better discriminatory power (169). In the literature, there are 17 references for studies that have used this genotyping method, almost all from Europe and mainly among ruminants (66,73,(170)(171)(172)(173)(174)(175)(176)(177)(178)(179)(180)(181)(182)(183)(184). The clone causing the Netherlands outbreak was identified as CbNL01 using this method, and a very similar clone was detected in goats in Belgium (178).…”

Section: Comparative Genomics and Pangenomic Analysismentioning

confidence: 99%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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Coxiella burnetii is the agent of Q fever, or "query fever," a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection with C. burnetii was the breaking of the old dichotomy between "acute" and "chronic" Q fever. The clinical presentation of C. burnetii infection depends on both the virulence of the infecting C. burnetii strain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients with C. burnetii infection.

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Section: Comparative Genomics and Pangenomic Analysismentioning

confidence: 99%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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“…A subsequent version (ACCM-2) supported improved growth and was shown to be effective for the virulent Nine Mile Phase 1 and Q212 C. burnetii isolates (Omsland et al 2011). It has been demonstrated that C. burnetii can be isolated from a clinical sample using ACCM-2 (Boden et al 2015), but whether ACCM-2 is effective for isolation of all contemporary sequence types is not known.…”

Section: Introductionmentioning

confidence: 99%

Genotyping and Axenic Growth ofCoxiella burnetiiIsolates Found in the United States Environment

Kersh

Priestley

Hornstra

et al. 2016

Vector-Borne and Zoonotic Diseases

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Coxiella burnetii is a gram-negative bacterium that is the etiologic agent of the zoonotic disease Q fever. Common reservoirs of C. burnetii include sheep, goats, and cattle. These animals shed C. burnetii into the environment, and humans are infected by inhalation of aerosols. A survey of 1622 environmental samples taken across the United States in 2006-2008 found that 23.8% of the samples contained C. burnetii DNA. To identify the strains circulating in the U.S. environment, DNA from these environmental samples was genotyped using an SNP-based approach to derive sequence types (ST) that are also compatible with multispacer sequence typing methods. Three different sequence types were observed in 31 samples taken from 19 locations. ST8 was associated with goats and ST20 with dairy cattle. ST16/26 was detected in locations with exposure to various animals and also in locations with no direct animal contact. Viable isolates were obtained for all three sequence types, but only the ST20 and ST16/26 isolates grew in acidified citrate cysteine medium (ACCM)-2 axenic media. Examination of a variety of isolates with different sequence types showed that ST8 and closely related isolates did not grow in ACCM-2. These results suggest that a limited number of C. burnetii sequence types are circulating in the U.S. environment and these strains have close associations with specific reservoir species. Growth in ACCM-2 may not be suitable for isolation of many C. burnetii strains.

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“…However, in a recent study, the first isolation of C. burnetii from a clinical specimen using a newly developed cell-free medium, acidified citrate cysteine medium 2 (ACCM2), was described. 39 The sensitivity of this medium is yet unknown, but results may modify the current investigative methods.…”

Section: Culturementioning

confidence: 99%

Q Fever (Coxiella Burnetii)

España

Uranga

Cillóniz

2020

Semin Respir Crit Care Med

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AbstractQ fever is a zoonotic infectious disease caused by the Coxiella burnetii bacterium. It is an obligate intracellular pathogen with a high infection capacity that proliferates exclusively in an acidified medium, forming a lysosome-like vacuole. It presents a peculiar phenomenon called “antigenic phase variation,” produced by a modification in the complexity of the membrane lipopolysaccharides. Q fever can be found worldwide and presents variable clinical features and geographical distribution. It mostly affects people in rural areas who are in contact with animals. The most common type of transmission to humans is via the inhalation of aerosols containing the pathogen, especially those formed from placental derivatives. Wild animals, domestic animals, and ticks are the principal reservoirs.Diagnosis is mainly made by indirect methods such as serology or by direct methods such as microbiological cultures or tests that detect the specific DNA. Typically, there are two clinical presentations: the acute disease, which is more frequent and often asymptomatic, and a persistent focalized infection in 4 to 5% of patients, generally with a poor evolution. Treatment of the acute form in both children and adults consists of administering doxycycline, while persistent focalized infection should be treated with at least two antibiotics, such as doxycycline and hydroxychloroquine. Several measures should be undertaken to minimize exposure among people working with animals or handling birth products. Different vaccines have been developed to prevent infection, though few data are available.

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First isolation of Coxiella burnetii from clinical material by cell-free medium (ACCM2)

Cited by 23 publications

References 22 publications

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

Genotyping and Axenic Growth ofCoxiella burnetiiIsolates Found in the United States Environment

Q Fever (Coxiella Burnetii)

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