First Isolation of Dietzia cinnamea from a Dog Bite Wound in an Adult Patient

Hirvonen, Jari J.; Lepistö, Inari; Mero, Sointu; Kaukoranta, Suvi-Sirkku

doi:10.1128/jcm.01939-12

Cited by 13 publications

(13 citation statements)

References 18 publications

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Section: Discussionmentioning

confidence: 99%

“…Virulence Tests With C. elegans and G. mellonella Because several other Dietzia isolates have been considered as potential pathogens based on their origins from immunocompromised patients (Hirvonen et al, 2012), we pursued studies to determine whether or not strain 55 exhibited virulence traits. Assays with the nematode C. elegans are frequently used to analyze broad-host range microbial pathogenicity when P. aeruginosa PA14, which is an effective killer of nematodes, is used as a positive control.…”

Section: Outside Garden Studiesmentioning

confidence: 99%

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Inoculation With a Microbe Isolated From the Negev Desert Enhances Corn Growth

et al. 2020

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Corn (Zea mays L.) is not only an important food source, but also has numerous uses, including for biofuels, fillers for cosmetics, glues, and so on. The amount of corn grown in the U.S. has significantly increased since the 1960's and with it, the demand for synthetic fertilizers and pesticides/fungicides to enhance its production. However, the downside of the continuous use of these products, especially N and P fertilizers, has been an increase in N 2 O emissions and other greenhouse gases into the atmosphere as well as runoff into waterways that fuel pollution and algal blooms. These approaches to agriculture, especially if exacerbated by climate change, will result in decreased soil health as well as human health. We searched for microbes from arid, native environments that are not being used for agriculture because we reasoned that indigenous microbes from such soils could promote plant growth and help restore degraded soils. Employing cultivation-dependent methods to isolate bacteria from the Negev Desert in Israel, we tested the effects of several microbial isolates on corn in both greenhouse and small field studies. One strain, Dietzia cinnamea 55, originally identified as Planomicrobium chinense, significantly enhanced corn growth over the uninoculated control in both greenhouse and outside garden experiments. We sequenced and analyzed the genome of this bacterial species to elucidate some of the mechanisms whereby D. cinnamea 55 promoted plant growth. In addition, to ensure the biosafety of this previously unknown plant growth promoting bacterial (PGPB) strain as a potential bioinoculant, we tested the survival and growth of Caenorhabditis elegans and Galleria mellonella (two animal virulence tests) as well as plants in response to D. cinnamea 55 inoculation. We also looked for genes for potential virulence determinants as well as for growth promotion.

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Section: Discussionmentioning

confidence: 99%

Section: Outside Garden Studiesmentioning

confidence: 99%

Inoculation With a Microbe Isolated From the Negev Desert Enhances Corn Growth

et al. 2020

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“…Other Dietzia strains have been used for bioremediation, for biosurfactants, bioemulsifiers, and carotenoid pigment production, and also as therapeutic probiotics for ruminant animals with paratuberculosis (Click and Van Kampen, 2010;Click, 2011;Procópio et al, 2013;Gharibazhedi et al, 2013). Nevertheless, some Dietzia strains have been reported to be opportunistic pathogens (Hirvonen et al, 2012), which is the reason we focused on finding potential virulence factors in strain 55. Surprisingly we could not detect obvious PGPB genes for plant growth promotion such as those found in other for phytohormone biosynthesis, other than the production of the polyamine spermidine, which interferes with ethylene biosynthesis (Maymon et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Inoculation with a microbe isolated from the Negev Desert enhances corn growth

Khan

iacutenez-Hidalgo

Humm

et al. 2019

Preprint

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Corn (Zea mays L.) is not only an important food source, but also has numerous uses, including for biofuels, fillers for cosmetics, glues, and so on. The amount of corn grown in the U.S. has significantly increased since the 1960s and with it, the demand for synthetic fertilizers and pesticides/fungicides to enhance its production. However, the downside of the continuous use of these products, especially N and P fertilizers, has been an increase in N2O emissions and other greenhouse gases into the atmosphere as well as run-off into waterways that fuel pollution and algal blooms. These approaches to agriculture, especially if exacerbated by climate change, will result in decreased soil health as well as human health. We searched for microbes from arid, native environments that are not being used for agriculture because we reasoned that indigenous microbes from such soils could promote plant growth and help restore degraded soils.Employing cultivation-dependent methods to isolate bacteria from the Negev Desert in Israel, we tested the effects of several microbial isolates on corn in both greenhouse and small field studies.One strain, Dietzia cinnamea 55, originally identified as Planomicrobium chinensis, significantly enhanced corn growth over the uninoculated control in both greenhouse and outside garden experiments. We sequenced and analyzed the genome of this bacterial species to elucidate some of the mechanisms whereby D.cinnamea 55 promoted plant growth. In addition, to ensure the biosafety of this previously unknown plant growth promoting bacterial (PGPB) strain as a potential bioinoculant, we tested the survival and growth of Caenorhabditis elegans (a test for virulence) in response to D. cinnamea 55. We also looked for genes for potential virulence determinants as well as for growth promotion.3

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“…This strain has also been detected from the skin of healthy individuals [ 17 ]. The strain D. cinnamea has been isolated from a perianal swab in a patient with bone marrow transplantation [ 18 ] and from a dog bite wound in an adult patient [ 19 ]. The authors in both case reports questioned the potential of D. cinnamea to cause infection.…”

Section: Discussionmentioning

confidence: 99%

Identification of Dietzia Species in a Patient with Endophthalmitis following Penetrating Injury with Retained Intraocular Metallic Foreign Body

Navaratnam

Dedi

Tjølsen

et al. 2018

Case Reports in Infectious Diseases

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To the best of our knowledge, we report the first case of Dietzia species in a patient with endophthalmitis. A 47-year-old man presented to the ophthalmology department with decreased vision, redness, and minimal pain in his right eye after a foreign body struck his eye following working using a hammer and chisel. Broad-spectrum polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) sequencing targeting 16S ribosomal ribonucleic acid-(rRNA-) encoding gene on an undiluted vitreous sample revealed 100% identity with GenBank sequences of Dietzia species including D. natronolimnaea, D. dagingensis, and D. cercidiphylli. The culture of the vitreous samples demonstrated the growth of Gram-positive cocci and polymorphic rods. The isolate from the culture was identified as D. natronolimnaea using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The combination of surgical and medical treatment (pars plana vitrectomy and systemic and topical antibiotics) eradicated the infection successfully.

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First Isolation of Dietzia cinnamea from a Dog Bite Wound in an Adult Patient

Abstract: We report the first case of deep-wound colonization by Dietzia cinnamea in a patient who had been bitten by a dog.

Cited by 13 publications

References 18 publications

Inoculation With a Microbe Isolated From the Negev Desert Enhances Corn Growth

Inoculation With a Microbe Isolated From the Negev Desert Enhances Corn Growth

Inoculation with a microbe isolated from the Negev Desert enhances corn growth

Identification of Dietzia Species in a Patient with Endophthalmitis following Penetrating Injury with Retained Intraocular Metallic Foreign Body

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