First isolation of polysaccharidic ulvans from the cell walls of freshwater algae

Pankiewicz, Radosław; Łęska, Bogusława; Messyasz, Beata; Fabrowska, Joanna; Sołoducha, M.; Pikosz, Marta

doi:10.1016/j.algal.2016.02.025

Cited by 42 publications

(20 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of sulfated polysaccharides in C. surera is in agreement with two recent studies on C. glomerata from different freshwater environments (Nan river in Thailand and Lake Oporzynskie in Poland) ( Pankiewicz et al, 2016 ; Surayot et al, 2016 ). In all three cases, they developed in freshwater environments.…”

Section: Resultssupporting

confidence: 92%

“…In an opposite way, very recently, it was found that, at least the green alga Cladophora glomerata and also Ulva flexuosa (Ulvophyceae, Chlorophyta), from two different freshwater environments (Nan river in Thailand and Lake Oporzynskie in Poland), with no detectable salt, were able to synthesize sulfated polysaccharides ( Pankiewicz et al, 2016 ; Surayot et al, 2016 ). In both investigations, although the presence of sulfated polysaccharides was well established, the fine structures of polysaccharides where these sulfate groups were detected, were not conclusive, and further studies are required to confirm these findings.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation

et al. 2017

View full text Add to dashboard Cite

The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment. Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants. Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses. Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units. Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected. In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized. It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus. Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor. Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength. Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Additional modifications of the procedure were performed separately to test other hypotheses or add data to the material balance (Table 1) and are discussed in detail in the following sections. [16] determined amount of sulfated polysaccharides and rounded up to 2 g per one 30 g extraction run. b Calcium citrate is better soluble in excess citric acid.…”

Section: Materials Balance Determinationmentioning

confidence: 99%

“…In our preliminary studies we have found that the mass obtained after evaporation of water extract obtained with a Soxhlet apparatus was a very modest 0.97 g per 100 g of dry biomass. That obviously meant that the bulk of sulfated polysaccharide (SP) fraction was left inside the thimble, as that fraction should amount to 5-10 % of dry mass [16]. The extract, however, was quite viscous, brown and showed a protein-rich FT-IR spectrum, with some bands that could be ascribed to sulfates and other salts -probably inorganic matter or small sulfated sugar molecules cleaved from the polysaccharide chain: all notable contaminants of SPs isolated by conventional means.…”

Section: Addition Of Edta and Isolation Of Sulfated Polysaccharides (mentioning

confidence: 99%

“…This is also described in following sections. The multi-step approach presented in this work allows for isolation of different potentially valuable fractions (including the sulfated polysaccharide fraction [16]), while simultaneously removing heavy metal ions -a common algal biomass contaminantin a selective manner, without sacrificing any important compounds in the process.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Material Balance for Stepwise Extraction of Freshwater Algae Biomass with Heavy Metal Sequestration

Piotrowicz

Tabisz

Łęska

2019

Ecological Chemistry and Engineering S

View full text Add to dashboard Cite

During our studies concerning the isolation of sulfated polysaccharides (SPs) from freshwater algae species widespread in Poland, an improved overall extraction methodology has been developed. This new, multi-step procedure allows for obtainment of several fractions that could find use in cosmetic and food industries, with the final one containing the valuable SPs, important both from economic and scientific perspective. Moreover, the process allows for easy use of typical chelating agents to remove harmful heavy metal ions without important losses in natural compounds of interest. Furthermore, a material balance has been established for said process, allowing for its easier implementation on bigger scales, and highlighting the areas which could still be improved to positively affect the final time-cost ratio of the methodology. Combined with previously published information concerning the detailed composition of obtained fractions, we aim to provide a robust and informative outlook on the potential of native freshwater algae species as cheap, raw and easily purifiable resource, usable in a number of important industries. According to the mass balance, nearly 5 % of dry mass of Cladophora glomerata is extractable with ethanol and this fraction consists mostly of fatty acids, phenolics and pigments. Another 5 % of mass can be isolated as pure SPs from aqueous fraction. Additionally, calcium from natural incrustations on the surface of C. glomerata amounts to 17 % of dry material weight and can be reclaimed from acidic wash by simple precipitation; such calcium salts have garnered significant interest as nutritional supplements.

show abstract

Biopolymers from Microbial Flora

Velamakanni

Sree²,

Vuppugalla

et al. 2022

Biopolymers

View full text Add to dashboard Cite

First isolation of polysaccharidic ulvans from the cell walls of freshwater algae

Cited by 42 publications

References 54 publications

Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation

Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation

Material Balance for Stepwise Extraction of Freshwater Algae Biomass with Heavy Metal Sequestration

Biopolymers from Microbial Flora

Contact Info

Product

Resources

About