First isolation of the enterohaemorrhagic Escherichia coli O145:H- from cattle in feedlot in Argentina

Padola, Nora Lía; Sanz, Marcelo E.; Lucchesi, Paula María Alejandra; Blanco, Jesús E.; Blanco, Jorge; Blanco, Miguel; Etcheverría, Analía Inés; Arroyo, Guillermo H; Parma, A

doi:10.1186/1471-2180-2-6

Cited by 19 publications

(7 citation statements)

References 23 publications

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“…Following individual O157 strain isolation, those samples positive for the eae-c gene were subjected to immunomagnetic separation (IMS), a rapid method used for detecting O157 strains. The methods described by Padola et al (2004) were followed. Samples were inoculated in peptone water supplemented with antibiotics (0.05 mg cefixime l 21 , 8 mg vancomycin l 21 , 10 mg cefsulodin l 21 ) and incubated with shaking at 37 uC for 6 h. One millilitre of each sample was then subjected to IMS with polystyrene magnetic beads coated with antibody specific for O157 (Dynal) as described by Lindqvist et al (1998).…”

Section: Bacteriological Procedures and Analysis Of Virulence Genesmentioning

confidence: 99%

Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Rivero

Passucci

Rodríguez

et al. 2010

Journal of Medical Microbiology

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The aim of this study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish this objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60 % of the children studied presented watery or mucous diarrhoea without blood, and in 25.2 % of the cases the samples contained blood. In a first screening, a multiplex PCR was performed to detect the presence of the vt 1 , vt 2 , eae, ehxA and saa virulence genes. The strains were then isolated and analysed to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty-four of the 437 samples (10.1 %) were positive for VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.3 % belonged to the non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157 : H7, O145 : H " , O26 : H11, O121 : H19, O111 : H2 and O118 : H2. HUS developed in 16 (36.4 %) patients positive for VTEC virulence genes. All of the VTEC isolates produced a cytopathic effect on Vero cell monolayers, confirming the ability to express VT. Despite most strains being sensitive to all of the antimicrobials studied, a positive association between clinical progression to HUS and antibiotic therapy was observed for the total number of patients studied, as well as for the VTEC + group. In conclusion, the data obtained in this study increase our knowledge of the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhoea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be using multiplex PCR to search for the presence of the vt 1 , vt 2 , eae and ehxA genes.

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Section: Bacteriological Procedures and Analysis Of Virulence Genesmentioning

confidence: 99%

Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Rivero

Passucci

Rodríguez

et al. 2010

Journal of Medical Microbiology

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“…In Colombia, E. coli O157 was isolated in 6.5 % of 307 rectal swabs from cattle (Mattar & Vásquez, 1998). The prevalence of E. coli O157 in healthy young beef steers and grazing-fed cattle in Argentina was 0.5 and 6.8 %, respectively (Meichtri et al, 2004;Padola et al, 2004), whilst similar studies in Brazil have shown rates of 0.6 % in dairy cattle (Irino et al, 2005), and 1.5 % in beef and dairy calves (Cerqueira et al, 1999). In developed countries, the prevalence rates of E. coli O157 : H7 vary considerably from 1.8 % in Japan (Miyao et al, 1998) to 23 % in the USA (Smith et al, 2001).…”

Section: Serum Bactericidal Activitymentioning

confidence: 99%

Common epitopes in LPS of different Enterobacteriaceae are associated with an immune response against Escherichia coli O157 in bovine serum samples

Navarro

Eslava

Torre

et al. 2007

Journal of Medical Microbiology

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Epidemiological studies in both humans and animals conducted in Mexico have shown that the isolation frequency of Escherichia coli O157 : H7 is low. In a previous study, IgG antibodies against E. coli O157, O7 and O116 LPS were found in serum samples from children and adults with no previous history of E. coli O157 : H7 infection. The present study was designed to determine whether a similar immune response against E. coli O157 : H7 and other antigenically related bacteria was present in bovine serum samples. A total of 310 serum samples from different herds in Mexico was analysed by microagglutination assays against different enterobacterial antigens, including E. coli O157. Microagglutination assays were positive against E. coli O7 (55 %), O116 (76 %) and O157 (36 %), Escherichia hermannii (15 %), Salmonella enterica serotype Urbana (14 %) and Salmonella enterica subsp. arizonae (40 %). These results were confirmed using a specific ELISA with purified LPS. A positive reaction was observed against the LPS of E. coli O7 (29 %), O116 (12 %) and O157 (22 %), E. hermannii (4 %), Salmonella Urbana (13 %) and S. enterica subsp. arizonae (12 %). Serum absorption studies of positive serum samples indicated the existence of at least three common epitopes shared by the LPS of E. coli O7, O116 and O157, and two others between E. coli O157 and Salmonella Urbana and S. enterica subsp. arizonae. A bactericidal assay against E. coli O157 : H7 using 31 bovine serum samples was performed, and 22 (71 %) of these serum samples gave positive results. The data demonstrated that bovine serum showed a response against different enterobacteria, including E. coli O157, and that this response could be due to the presence of shared epitopes in the LPS of these organisms. INTRODUCTIONEscherichia coli O157 : H7 infection and the consequent associated diseases constitute an important health problem for children and the elderly, mainly in developed countries (Bitzan et al., 1993;Gillespie et al., 2005;Griffin & Tauxe, 1991;Rangel et al., 2005). Cattle and sheep are natural reservoirs, and are also important transmission vectors for these bacteria (Chapman et al., 1997;Hancock et al., 1997). Isolation rates have been widely reported (Naylor et al., 2005), with levels of 1.8 % in Japan (Miyao et al., 1998), 1.9 % in Australia (Cobbold & Desmarchelier, 2000) and from 0 to 7.4 % in the USA (Faith et al., 1996). In Mexico, serotyping data of approximately 16 000 E. coli isolates collected over the past 20 years have shown an isolation frequency of 1 % for E. coli O157 strains (Navarro et al., 2003). In another study in Mexico, Callaway et al. (2004) showed an isolation frequency of 1.25 % for E. coli O157 in cattle and 2.1 % in swine. Studies conducted in countries with defined outbreaks of haemolytic uraemic syndrome or haemorrhagic colitis associated with the presence of E. et al., 1986) has revealed that a common structural epitope consisting of Nacetyl derivatives of 4-amino-4,6-dideoxy-a-D-mannopyranosyl residues is present in each respe...

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“…A total of 95 strains were selected from a well characterized, previously described strain collection of our Laboratory. To assess potential associations among subA, other virulence factors and serotypes, we selected representative strains taking into account the presence of vt 1 , vt 2 , eae, saa, and ehxA genes determined in previous studies (15,16,22). In the present work, a previously described multiplex PCR, which detects subA, vt 1 and vt 2 , was used for subA screening (20).…”

mentioning

confidence: 99%

Occurrence of subtilase cytotoxin and relation with other virulence factors in verocytotoxigenic Escherichia coli isolated from food and cattle in Argentina

Velandia¹,

Sanso²,

Krüger³

et al. 2011

Braz. J. Microbiol.

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First isolation of the enterohaemorrhagic Escherichia coli O145:H- from cattle in feedlot in Argentina

Cited by 19 publications

References 23 publications

Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Common epitopes in LPS of different Enterobacteriaceae are associated with an immune response against Escherichia coli O157 in bovine serum samples

Occurrence of subtilase cytotoxin and relation with other virulence factors in verocytotoxigenic Escherichia coli isolated from food and cattle in Argentina

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