First molecular characterisation of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) in Victoria, Australia

Abeywardena, Harshanie; Jex, Aaron R.; Samson‐Himmelstjerna, Georg von; Haydon, Shane R.; Stevens, Melita; Gasser, Robin B.

doi:10.1016/j.meegid.2013.07.019

Cited by 32 publications

(62 citation statements)

References 58 publications

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“…parvum was the predominant species in young buffaloes (Cacciò et al 2007;Maurya et al 2013;Inpankaew et al 2014), and in the previous study in Australia, C. parvum was only detected in buffalo <6 months of age (Abeywardena et al 2013a). In the present study, however, all buffalo samples were adults (2-5 years), indicating that this species can also commonly infect adult buffaloes.…”

Section: Discussioncontrasting

confidence: 57%

“…This species has been detected previously in buffaloes in many countries including Spain, Italy, Australia, Egypt, India, and Thailand (Amer et al 2013;Cacciò et al 2007;Gómez-Couso et al 2005;Maurya et al 2013;Abeywardena et al 2013a;Inpankaew et al 2014;Mahfouz et al 2014;Aquino et al 015). Previous studies have reported that C.…”

Section: Discussionmentioning

confidence: 62%

“…The prevalence of Cryptosporidium species in the farmed and wild buffalo fecal samples was 30.0 and 12.0 % respectively. A previous study of Cryptosporidium in farmed water buffalo in Victoria reported a prevalence of 13.0 % (62/476) (Abeywardena et al 2013a). Other studies have reported prevalences in buffalo ranging from 5.7 to 62.1 % (Amer et al 2013;Inpankaew et al 2014;Abeywardena et al 2014;Aquino et al 2015;Ma et al 2015).…”

Section: Discussionmentioning

confidence: 95%

“…However, relatively little is known about the range of species and genotypes of Cryptosporidium in other members of the family Bovidae, including water buffalo (Bubalus bubalis), but to date, C. parvum, C. ryanae, C. bovis, C. ubiquitum, a "C. suis-like" genotype, and C. ryanae variants have been reported (Gómez-Couso et al 2005;Cacciò et al 2007;Feng et al 2012;Venu et al 2012;Abeywardena et al 2013a;Abu Samra et al 2013;Amer et al 2013;Helmy et al 2013;Inpankaew et al 2014;Mahfouz et al2014;Ma et al 2015;Abeywardena et al 2015;Aquino et al 2015;Helmy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…To date, only one study of Cryptosporidium in farmed water buffalo in Victoria, Australia, has been conducted (Abeywardena et al 2013a), and nothing is known about the prevalence and species of Cryptosporidium infecting water buffalo in Northern Australia. Therefore, the aim of the present study was to use molecular tools to better understand the public health risks of Cryptosporidium sp.…”

Section: Introductionmentioning

confidence: 99%

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First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

Zahedi

Phasey²,

Boland³

et al. 2016

Parasitol Res

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A molecular epidemiological survey of Cryptosporidium from water buffalo (Bubalus bubalis) in the Northern Territory in Australia was conducted. Fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18S quantitative PCR (qPCR). Positives were typed by sequence analysis of 18S nested PCR products. The qPCR prevalence of Cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. Sequence analysis identified two species: C. parvum and C. bovis, with C. parvum accounting for ~80 % of positives typed from the farmed buffalo fecal samples compared to 50 % for wild buffalo. Subtyping at the 60 kDa glycoprotein (gp60) locus identified C. parvum subtypes IIdA19G1 (n = 4) and IIdA15G1 (n = 1) in the farmed buffalo and IIaA18G3R1 (n = 2) in the wild buffalo. The presence of C. parvum, which commonly infects humans, suggests that water buffaloes may contribute to contamination of rivers and waterways with human infectious Cryptosporidium oocysts, and further research on the epidemiology of Cryptosporidium in buffalo populations in Australia is required.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

Zahedi

Phasey²,

Boland³

et al. 2016

Parasitol Res

View full text Add to dashboard Cite

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Molecular identification of the Cryptosporidium deer genotype in the Hokkaido sika deer (Cervus nippon yesoensis) in Hokkaido, Japan

et al. 2015

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The protozoan Cryptosporidium occurs in a wide range of animal species including many Cervidae species. Fecal samples collected from the Hokkaido sika deer (Cervus nippon yesoensis), a native deer of Hokkaido, in the central, western, and eastern areas of Hokkaido were examined by polymerase chain reaction (PCR) to detect infections with Cryptosporidium and for sequence analyses to reveal the molecular characteristics of the amplified DNA. DNA was extracted from 319 fecal samples and examined with PCR using primers for small-subunit ribosomal RNA (SSU-rRNA), actin, and 70-kDa heat shock protein (HSP70) gene loci. PCR-amplified fragments were sequenced and phylogenetic trees were created. In 319 fecal samples, 25 samples (7.8 %) were positive with SSU-rRNA PCR that were identified as the Cryptosporidium deer genotype. Among Cryptosporidium-positive samples, fawns showed higher prevalence (16.1 %) than yearlings (6.4 %) and adults (4.7 %). The result of Fisher's exact test showed a statistical significance in the prevalence of the Cryptosporidium deer genotype between fawn and other age groups. Sequence analyses with actin and HSP70 gene fragments confirmed the SSU-rRNA result, and there were no sequence diversities observed. The Cryptosporidium deer genotype appears to be the prevalent Cryptosporidium species in the wild sika deer in Hokkaido, Japan.

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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1–2-month-old highland yaks in Qinghai Province, China

Wang

et al. 2018

Parasitol Res

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Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, China. Cryptosporidium spp. and Giardia duodenalis were detected by light and immunofluorescence microscopy and nested PCR (nPCR). Fifteen samples were positive (4.5%) by Kinyoun staining, 40 (11.6%) samples were positive by immunofluorescence test (IFT), and 39 (11.3%) samples were positive by nPCR for Cryptosporidium spp., Cryptosporidium bovis (11/39, 28.2%) was the most prevalent species, followed by C. ryanae (6/39, 15.4%), C. andersoni (5/39, 12.8%), C. struthionis (5/39, 12.8%), C. parvum (5/39, 12.8%), C. hominis (4/39, 10.3%) and C. canis (3/39, 7.7%). Thirteen out of 344 (3.8%) samples were positive for Giardia by simple microscopy, 20 (5.8%) by IFT and 18 samples (5.2%) yak faecal samples were Giardia positive by nPCR. Two G. duodenalis assemblages (B, E) were detected in this study. Nine positive samples for G. duodenalis assemblage E were from the towns of Xiewu (8/9, 4.9%) and Xiangda (1/9, 1.3%), and nine positive samples (9/9, 8.5%) for G. duodenalis assemblage B were from the town of Zhenqin. This report provides information about infection with Cryptosporidium species and G. duodenalis assemblages in domesticated 1-2-month-old highland yaks living in the Qinghai-Tibet Plateau region of China.

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First molecular characterisation of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) in Victoria, Australia

Cited by 32 publications

References 58 publications

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

Molecular identification of the Cryptosporidium deer genotype in the Hokkaido sika deer (Cervus nippon yesoensis) in Hokkaido, Japan

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1–2-month-old highland yaks in Qinghai Province, China

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