First molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a gram negative septicaemia

Reis, Mário; Vale, Ana do; Pinto, Carlos Felipe; Nascimento, Diana S.; Costa-Ramos, Carolina; Silva, Daniela S.; Silva, Manuel T.; Santos, Nuno M.S. dos

doi:10.1016/j.molimm.2006.07.293

Cited by 44 publications

(39 citation statements)

References 56 publications

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“…A similar tendency of caspase-9 mRNA change to caspase-3 was observed in the infected head kidneys in sea bass [24]. In the previous study, the enzyme activity of large yellow croaker caspase-9 (Lyccasp9) in the spleen and kidney was shown to be increased when the fish was stimulated with the poly(I:C) or bacterial vaccine [1].…”

Section: Discussionsupporting

confidence: 74%

Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea)

Ding

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 74%

Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea)

Ding

et al. 2011

Fish & Shellfish Immunology

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“…Such interactions have been shown to activate caspases. The morphological and biochemical changes associated with apoptosis are largely caused by activation of a family of intracellular cysteine aspartyl-specific proteases known as caspases, which cleave many vital cellular substrates contributing to cell death and to the apoptotic phenotype (35). Caspase-9 is an initiator caspase in the apoptotic process whose function is to activate effector caspases that occur downstream in the mitochondrial pathway of apoptosis (35).…”

Section: Discussionmentioning

confidence: 99%

Effects of apicidin, a histone deacetylase inhibitor, on the regulation of apoptosis in H-ras-transformed breast epithelial cells

Park

Im²,

Kim³

et al. 2008

Int J Mol Med

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Abstract. The cellular susceptibility of cancer cells to histone deacetylase (HDAC) inhibitors is increased by the etopic expression of oncogenic Ras. However, the ability of HDAC inhibitors to regulate the apoptotic pathway in human breast cancer cells is still not completely understood. In this study, the anti-proliferative effects of apicidin were compared in Hras-transformed human breast epithelial (MCF10A-ras) and non-transformed epithelial (MCF10A) cells. MCF10A-ras cells showed a significantly higher growth rate than MCF10A cells. Apicidin significantly increased the levels of acetylated histone H3 and H4 in both cell lines. Western blot analysis and flow cytometry were used to determine if the anti-proliferative effects of apicidin in MCF10A and MCF10A-ras cells could be mediated by modulating the cell cycle. Apicidin attenuated the expression of cyclin E and CDK2 in MCF10A cells, decreased cyclin D1 and cyclin E levels in MCF10A-ras cells, and increased the levels of CDK inhibitors, p21WAF1/Cip1 and p27Kip1 , in both cell lines. Notably, the levels of hyperphosphorylation of the Rb protein levels were lower in the MCF10A-ras cells after apicidin treatment. Studies on the regulation of apoptosis showed that apicidin induces the upregulation of p53 and the downstream activation of ERK in MCF10A-ras cells. The up-regulation of p53 promoted Bax expression leading to activation of caspases-9 and -6, and eventually to apoptosis in MCF10A-ras cells. In addition, apicidin significantly increased the levels of ERK1/2 phosphorylation in MCF10A-ras cells. Therefore, the apicidinmediated ERK pathway appears to play an important role in modulating the pro-apoptotic pathway in MCF10A-ras cells.

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“…One study showed that injection of mRNA encoding zebrafish caspase-9 induced apoptosis and embryonic death, although to a lesser degree compared to the same amount of caspase-8 [16]. In sea bass, caspase-9 expression was found to be very low under normal conditions in most organs, but it was significantly enhanced in head kidney upon bacterial infection, where its expression was associated with morphological alterations typical of apoptosis [60]. Both studies thus are consistent with the notion that caspase-9 is involved in apoptosis of fish cells.…”

Section: Fish Caspases Their Activators and Inhibitorsmentioning

confidence: 99%

Fish as model systems for the study of vertebrate apoptosis

Krumschnabel

Podrabsky

2008

Apoptosis

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Apoptosis is a process of pivotal importance for multi-cellular organisms and due to its implication in the development of cancer and degenerative disease it is intensively studied in humans and mammalian model systems. Invertebrate models of apoptosis have been well-studied, especially in C. elegans and D. melanogaster, but as these are evolutionarily distant from mammals the relevance of findings for human research is sometimes limited. Presently, a non-mammalian vertebrate model for studying apoptosis is missing. However, in the past few years an increasing number of studies on cell death in fish have been published and thus new model systems may emerge. This review aims at highlighting the most important of these findings, showing similarities and dissimilarities between fish and mammals, and will suggest topics for future research. In addition, the outstanding usefulness of fish as research models will be pointed out, hoping to spark future research on this exciting, often underrated group of vertebrates.

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First molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a gram negative septicaemia

Cited by 44 publications

References 56 publications

Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea)

Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea)

Effects of apicidin, a histone deacetylase inhibitor, on the regulation of apoptosis in H-ras-transformed breast epithelial cells

Fish as model systems for the study of vertebrate apoptosis

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