First molecular data on Eimeria truttae from brown trout (Salmo trutta)

Mixed Eimeria and Cryptosporidium infection and its effects on pathology and clinical outcomes in juvenile Asian seabass (Lates calcarifer) cultured in Thailand

“…Both Eimeria and Cryptosporidium colonization in the intestinal system of Asian seabass were evident by microscopic observation and PCR amplification of the 18S rRNA gene. Phylogenetic analysis in this study showed the considerable genetic distance between piscine‐ and other higher vertebrate host‐derived isolates of Cryptosporidium and Eimeria species, which was consistent with previous studies (Couso‐Pérez et al., 2019, 2022; Ryan et al., 2015; Zanguee et al., 2010). Thai Cryptosporidium sp.…”

First report of Eimeria myoxi in the garden dormouse (Eliomys quercinus Linnaeus, 1766) from Doñana Natural Area (Andalusia, SW Spain)

“…Eimeria species described in rodents. Amplification and sequencing of the SSU-rRNA, ORF470 and COI genes are currently being used to detect and characterise Eimeria in multiple host species [16,[27][28][29][30]. In the present study, molecular analysis of a fragment of the SSU-rRNA gene confirmed that the oocysts visualized in 15 faecal samples from E. quercinus belong to E. myoxi and allowed to identify this coccidian species in other seven garden dormouse samples, increasing the prevalence value obtained by microscopical examination.…”

“…Genomic DNA was extracted from approximately 0.5 g of each faecal sample with the Stool DNA Isolation Kit (Norgen Biotek Corp., Thorold, ON, Canada) according to the manufacturer's instructions. Samples were screened using PCR techniques which amplified fragments of ~420 bp, ~ 810 bp and ~ 780 bp of the small subunit ribosomal RNA (SSU-rRNA), the apicoplast open reading frame 470 (ORF470) and the mitochondrial cytochrome c oxidase subunit I (COI) genes of Eimeria, respectively [16][17][18]. The amplicons were electrophoresed on 2% agarose gels and stained with Real Safe (Real Laboratory S.L., Paterna, Valencia, Spain).…”

Molecular identification of Eimeria species in Spanish bats

“…The faecal samples were processed using a previously described diphasic concentration method [12]. Briefly, 0.1-0.5 g of each sample was individually ground in a mortar with 30 mL of 0.04 M phosphate buffered saline (PBS) pH 7.2, filtered through a set of two sieves (150 and 45 μm of mesh size), shaken vigorously with diethyl ether (2:1) and finally concentrated by centrifugation at 1250 ×g, 4 • C, for 15 min.…”

“…The extracted DNA was stored at − 20 • C until use. A heminested PCR technique was used to amplify a ~ 420 bp fragment of the SSU-rRNA gene of Eimeria [12]. In all PCR runs, genomic DNA of Eimeria tenella from naturally infected chickens was used as a positive control, and molecular biology grade water was used as a negative control.…”