First Molecular Detection and Vp7 (G) Genotyping of Group a Rotavirus by Semi-Nested Rt-PCR From Sewage in Nigeria

Motayo, Babatunde Olanrewaju; Ja, Aznar; Faneye, Adedayo Omotayo

doi:10.1590/s1678-9946201658074

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…During this time seven VP7 genotypes were reported from Nigeria, genotypes G1, G2, G3, G8, G9, G10, and G12, with genotypes G9 and G12 being the most recent isolates [ 22 – 24 , 26 – 29 ]. Environmental isolates recovered from sewage effluent were also identified in our analysis [ 42 ]. Our analysis shows a rapid emergence and dominance of genotype G12 lineage III in Nigeria.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Rotavirus A in Nigeria: Molecular Diversity and Current Insights

Motayo

Faneye

Adeniji

2018

Journal of Pathogens

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Rotavirus induced acute gastroenteritis AGE has been a major disease burden in Nigeria, since it was first reported in 1985. Prevalence rates have increased with severe public health consequences particularly among children. The vaccine Rotarix® has been introduced and is commercially available in Nigeria. However routine rotavirus vaccination is yet to be introduced into the National Immunization Program. Molecular epidemiology of rotavirus in Nigeria has shown the presence of various genotypes, with genotype G12P[8] being the most recent introduction. There are however gaps in molecular data on rotavirus in Nigeria. We therefore reviewed molecular data on rotavirus isolated in Nigeria and also analyzed VP4 and VP7 genes of Nigerian rotavirus strains in Genbank. We have shown that there is a distinct trend in rotavirus molecular epidemiology in Nigeria, with new genotype introductions occurring after the year 2010. We also observed from our analysis the emergence of genotype G12 Lineage III as a dominant genotype. This information elucidates rotavirus molecular epidemiology in Nigeria and gives insight to the expanding landscape of rotavirus genotypes. We recommend the institution of molecular surveillance country wide, before considering the inclusion of rotavirus vaccination into the National Immunization Program in Nigeria, in other to monitor evolution of divergent or recombinant strains.

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Section: Discussionmentioning

confidence: 99%

Epidemiology of Rotavirus A in Nigeria: Molecular Diversity and Current Insights

Motayo

Faneye

Adeniji

2018

Journal of Pathogens

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“…Some articles assessed the presence of poliovirus, [69][70][71][72] either wildtype or vaccine-derived, including potential neurovirulence of vaccine-derived poliovirus. [73][74][75] Many articles documented the diversity of non-polio enteroviruses found in wastewater, 73,[76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92] with a variety of focuses including rotavirus, [93][94][95][96][97] norovirus, 88,92,[98][99][100] astrovirus, 101,102 polyomavirus, 103 Saffold virus, 104 hepatitis A virus, 85 hepatitis E virus, 105 mastadenovirus, 106 Aichi virus, 107 and human bocavirus. 107 Surveillance of Giardia and/or Cryptosporidium was also documented.…”

Section: Figure 2 Global Distribution Of Studies Of Wastewater Surveillance For Infectious Diseasementioning

confidence: 99%

Wastewater surveillance for infectious disease: a systematic review

Kilaru

Hill

Anderson³

et al. 2021

Preprint

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Wastewater surveillance of SARS-CoV-2 has shown to be a valuable source of information regarding SARS-CoV-2 transmission and COVID-19 cases. Though the method has been used for several decades to track other infectious diseases, there has not been a comprehensive review outlining all of the pathogens surveilled through wastewater. The aim of this study is to identify what infectious diseases have been previously studied via wastewater surveillance prior to the COVID-19 Pandemic and identify common characteristics between the studies, as well as identify current gaps in knowledge. Peer-reviewed articles published as of August 1, 2020 that examined wastewater for communicable and infectious human pathogens on 2 or more occasions were included in the study. Excluded from this list were all reviews and methods papers, single collection studies, and non-human pathogens. Infectious diseases and pathogens were identified in studies of wastewater surveillance, as well as themes of how wastewater surveillance and other measures of disease transmission were linked. This review did not include any numerical data from individual studies and thus no statistical analysis was done. 1005 articles were identified but only 100 were included in this review after applying the inclusion criteria. These studies came from 38 countries with concentration in certain countries including Italy, Israel, Brazil, Japan, and China. Twenty-five separate pathogen families were identified in the included studies, with the majority of studies examining pathogens from the family Picornaviridae, including polio and non-polio enteroviruses. Most studies of wastewater surveillance did not link what was found in the wastewater to other measures of disease transmission. Among those studies that did compare wastewater surveillance to other measures of disease transmission the value observed was dependent upon pathogen and varied by study. Wastewater surveillance has historically been used to assess water-borne and fecal-orally transmitted pathogens causing diarrheal disease. However, numerous other types of pathogens have been surveilled using wastewater and wastewater surveillance should be considered as a potential tool for many infectious diseases. Wastewater surveillance studies can be improved by incorporating other measures of disease transmission at the population-level including disease incidence and hospitalizations.

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“…However, rotavirus still causes about 192,700 deaths per year in children ˂5 years mostly in low-and middle-income countries [6]. In Nigeria, they have been reports of various genotypes including mixed and unusual genotype combination isolated from young children and environmental samples [7,8,9]. This might have undermined the public health efforts in controlling pediatric rotavirus infection in Nigeria.…”

Section: Introductionmentioning

confidence: 99%

Environmental Survey of Rotavirus in Sewage Water within Maiduguri Metropolis, Nigeria

Kadai

Bello

Ghamba

et al. 2020

Afro-Egyptian Journal of Infectious and Endemic Diseases

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Backgroundand study aim: Inadequately treated water supply and unhygienic food consumption are the major sources of contracting feco-orally transmitted viral pathogens, including Rotavirus. This prospective study aimed to assess the presence of Rotavirus in sewage, underground and surface waters collected from different locations within Maiduguri Metropolis, Nigeria. Materials and Methods:A total of 170 sewage and water samples (underground and surface waters) were collected from various sources in high-density and lowdensity areas of the Maiduguri metropolis. A modified adsorption-elution method for concentration of enteric viruses in sewage and water samples and detection of the presence of rotavirus (if present) was done using Enzyme linked immunesorbent assay (ELISA). The viruses in water were concentrated by negatively charged membrane filtration, eluted with 0.05M Glycine at pH 11.5, and reconcentrated by centrifugation at 12,000rpm for one hour.Results: Out of the 60 domestic sewage samples collected from high-density areas, 8.3% were positive for rotavirus antigen, whereas none of the samples from low-density areas were positive. In addition, rotavirus antigen was not detected in underground and surface water samples. The temperature range of the 5 rotavirus positive samples was 23-30 o C. All the 5 positive samples had alkaline pH that ranged between 8 and 9. Conclusion:This study has demonstrated the persistence of rotavirus in sewage and its possible transmission through contaminated food and poor treated water especially in unvaccinated children.

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First Molecular Detection and Vp7 (G) Genotyping of Group a Rotavirus by Semi-Nested Rt-PCR From Sewage in Nigeria

Cited by 13 publications

References 25 publications

Epidemiology of Rotavirus A in Nigeria: Molecular Diversity and Current Insights

Epidemiology of Rotavirus A in Nigeria: Molecular Diversity and Current Insights

Wastewater surveillance for infectious disease: a systematic review

Environmental Survey of Rotavirus in Sewage Water within Maiduguri Metropolis, Nigeria

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