First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

He, Li; Miao, Xiaoyan; Hu, Jinfang; Huang, Yuan; He, Pei; He, Junwei; Yu, Liang; Malobi, Ngabu; Shi, Liangrong; Zhao, Junlong

doi:10.3389/fmicb.2017.01577

Cited by 27 publications

(24 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of investigations of canine piroplasmosis have been performed during the spring or autumn, the seasons of highest vector tick activity. It has been previously reported in China that the prevalence of B. gibsoni and B. vogeli is 1.5–11.86% and 1.2–11.3%, respectively [ 9 , 18 , 30 , 33 ]. In 2019, 130 blood samples were randomly collected from pet dogs in Henan Province to investigate the infection status of piroplasms during the winter.…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence of piroplasm infection in companion animals in Hunan Province, China

Wang

Sun

et al. 2020

BMC Vet Res

View full text Add to dashboard Cite

Background Feline and canine babesiosis is an important tick-borne disease caused by parasites of the genus Babesia. The disease has a worldwide distribution and causes serious health problems in domestic and wild canidae and felidae. Results Genomic DNA was isolated from blood samples, which were randomly collected from pet dogs ( n = 115) and cats ( n = 25) in Changsha city of Hunan Province, China. Results of nested PCR assay targeting 18S rRNA gene and partial gene sequencing revealed that seven animals were infected with Babesia species, five dogs (5/115, 4.3%) and two cats (2/25, 8.0%). Sequence analysis showed that four dogs (3.5%) were positive for Babesia canis , and the other one for Babesia vogeli (0.87%). The two cats were infected by Babesia hongkongensis. Conclusions The findings of this study will expand knowledge of the distribution of Babesia species and provide important epidemiological information for the control of animal babesiosis in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular evidence of piroplasm infection in companion animals in Hunan Province, China

Wang

Sun

et al. 2020

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…Finally, if one considers the global distribution of B. gibsoni , some epidemiological findings need to be mentioned. Babesia gibsoni is widespread throughout the Oriental region, for instance in China [ 43 ]. However, cases of canine babesiosis caused by B. gibsoni have been reported outside the distributional range of its main vector tick, H. longicornis , incriminating other ticks for its transmission.…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence for the transovarial passage of Babesia gibsoni in Haemaphysalis hystricis (Acari: Ixodidae) ticks from Taiwan: a novel vector for canine babesiosis

Jongejan

Yang

et al. 2018

Parasites Vectors

View full text Add to dashboard Cite

BackgroundBabesia gibsoni is the predominant tick-borne protozoan blood parasite affecting dogs throughout the Oriental region. Babesia gibsoni is transmitted by Haemaphysalis longicornis, whereas a similar role has been suggested for Rhipicephalus sanguineus. Haemaphysalis longicornis does not occur in Taiwan, but R. sanguineus is widely distributed on dogs. However, clinical cases of babesiosis are mainly restricted to the northern part of the island. The discrepancy between tick distribution and clinical cases stimulated us to investigate the tick species distribution on dogs in northern Taiwan, with the aim to identify the local vector for canine babesiosis.MethodsTicks were collected from stray dogs or free ranging pet dogs in northern Taiwan between 2015 and 2017 and, after identification, were tested for the presence of tick-borne Babesia parasites using PCR and reverse line blot (RLB) hybridisation. Moreover, engorged ticks collected from the dogs were incubated at 28 °C to allow them to oviposit. Their subsequent larval progeny was also examined by PCR/RLB.ResultsA total of 1085 ticks collected from 144 stray dogs at different residential areas consisted of 5 different species: H. hystricis (n = 435), R. sanguineus (n = 582), R. haemaphysaloides (n = 43), Amblyomma testudinarium (n = 14) and Ixodes ovatus (n = 11) were identified. Babesia gibsoni DNA was detected in H. hystricis females (10.3%), males (7.0%) and in 2.6% of the nymphs. One R. sanguineus female and one A. testudinarium female tick also carried B. gibsoni DNA. DNA of B. gibsoni was demonstrated in 11 out of 68 (16.2%) batches of larval ticks derived from engorged H. hystricus ticks only. Babesia vogeli DNA was detected only in R. sanguineus females (2.6%) and males (2.4%). DNA of B. vogeli was detected in 13 out of 95 (13.7%) batches of larval ticks derived from engorged R.sanguineus females.ConclusionsBabesia gibsoni DNA was detected in the larval progeny of H. hystricis ticks only, whereas B. vogeli was restricted to the larvae of R. sanguineus. This provides evidence for transovarial passage of B. gibsoni in H. hystricis and evidence that this tick does act as the local vector for this parasite on dogs in northern Taiwan where most cases of babesiosis are reported. The vectorial capacity of R. sanguineus for babesiosis is probably restricted to the transmission of B. vogeli only.

show abstract

“…Studies have shown that it can spread through ticks, blood transfusion, and placenta (2, 3). In 2017, He et al reported the strain of B. gibsoni in Wuhan and provided the molecular biological evidence such as 18S rRNA and ITS gene sequence, confirming the epidemic of Babesia in Wuhan from the evolutionary point of view (4).…”

Section: Introductionmentioning

confidence: 94%

Evaluation of Babesia gibsoni GPI-anchored Protein 47 (BgGPI47-WH) as a Potential Diagnostic Antigen by Enzyme-Linked Immunosorbent Assay

Zhan

et al. 2019

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Babesia gibsoni is one of the important pathogens causing severe incurable canine babesiosis, suggesting the necessity to develop a sensitive, specific, and highly automated diagnostic method for clinical application. Surface proteins are ideal candidates for diagnostic targets because they are the primary targets for host immune responses during host-parasite interactions. Glycosylphosphatidylinositol (GPI)-anchored proteins are abundant on the surface of parasites and play an important role in parasite diagnosis. In this study, a GPI-anchored protein named BgGPI47-WH was obtained and mouse anti-rBgGPI47-WH polyclonal antibody was produced by immunizing mice with the purified protein and Freund's adjuvant. Western blot was used to identify the native form and immunogenicity of BgGPI47-WH. An ELISA method was established by using recombinant BgGPI47-WH protein to evaluate its potential as a diagnostic antigen and the established method exhibited high specificity. The antibody response was evaluated by using the B. gibsoni-infected sera collected from different experimental dogs and the established ELISA could recognize antibodies at day 6 until day 101 post infection, indicating the potential use of BgGPI47-WH for early stage diagnosis. The specificity of the established ELISA was further evaluated by using 147 clinical samples collected from animal hospitals and 17.0% (25/147) of the samples were tested positive, with an overall proportion agreement of 86.39% between the results from BgGPI47-WH and BgSA1. Our results indicated that BgGPI47-WH could be used as a reliable diagnostic antigen and this study has proposed a practical method for early diagnosis of B. gibsoni.

show abstract

First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

Cited by 27 publications

References 39 publications

Molecular evidence of piroplasm infection in companion animals in Hunan Province, China

Molecular evidence of piroplasm infection in companion animals in Hunan Province, China

Molecular evidence for the transovarial passage of Babesia gibsoni in Haemaphysalis hystricis (Acari: Ixodidae) ticks from Taiwan: a novel vector for canine babesiosis

Evaluation of Babesia gibsoni GPI-anchored Protein 47 (BgGPI47-WH) as a Potential Diagnostic Antigen by Enzyme-Linked Immunosorbent Assay

Contact Info

Product

Resources

About