First nanoplate digital PCR method to trace allergenic foods: Improved sensitivity for the detection of sesame

Villa, Caterina; Costa, Joana; Mafra, Isabel

doi:10.1016/j.foodchem.2024.138650

Food Chemistry

2024

DOI: 10.1016/j.foodchem.2024.138650

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First nanoplate digital PCR method to trace allergenic foods: Improved sensitivity for the detection of sesame

Caterina Villa,

Joana Costa,

Isabel Mafra

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Cited by 3 publications

References 26 publications

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Research progress on detection methods for food allergens

Zhang,

Lei,

Zou

et al. 2025

Journal of Food Composition and Analysis

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Research progress on detection methods for food allergens

Zhang,

Lei,

Zou

et al. 2025

Journal of Food Composition and Analysis

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Screening, identification, and application of aptamers against allergens in food matrices

Zhu,

Du,

Liu

et al. 2024

Critical Reviews in Food Science and Nutrition

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Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach

Llano-Suárez,

Sánchez-Visedo,

Ortiz-Gómez

et al. 2024

Biosensors

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Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation.

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First nanoplate digital PCR method to trace allergenic foods: Improved sensitivity for the detection of sesame

Cited by 3 publications

References 26 publications

Research progress on detection methods for food allergens

Research progress on detection methods for food allergens

Screening, identification, and application of aptamers against allergens in food matrices

Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach

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