First Organisms with Acquired Metallo-β-Lactamases (IMP-13, IMP-22, and VIM-2) Reported in Austria

Duljasz, Wojciech; Gniadkowski, Marek; Sitter, Silvia; Wojna, Alexandra; Jebelean, C.

doi:10.1128/aac.01573-08

Cited by 29 publications

(18 citation statements)

References 17 publications

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“…Two previously described clonal complexes, CC111 (previously described as CC4/BG4) and CC235 (previously described as CC11/BG11), have been shown to dominate among MBLproducing P. aeruginosa isolates (7,8,12,15,18,20,33), and the same CCs dominate in the isolates from Norway and Sweden. All isolates from Skåne County, along with isolates imported from Denmark and Greece, were of serotype O12 and displayed ST111/ST229, which is part of CC111, and all harbored bla VIM-2 as part of In59 variants.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, VIM-2 has emerged as a dominant MBL variant worldwide. Multilocus sequence typing (MLST) has identified international clonal complexes (CCs) responsible for the dissemination of MBL-producing Pseudomonas aeruginosa, particularly in European countries (7,8,12,18,20,33), but also in Japan (15), Singapore, and Brazil (http: //pubmlst.org/paeruginosa/).…”

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confidence: 99%

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Molecular Epidemiology of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa Isolates from Norway and Sweden Shows Import of International Clones and Local Clonal Expansion

Samuelsen

Toleman

Sundsfjord

et al. 2010

Antimicrob Agents Chemother

153

120

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Scandinavia is considered a region with a low prevalence of antimicrobial resistance. However, the number of multidrug-resistant (MDR) Gram-negative bacteria is increasing, including metallo-␤-lactamase (MBL)-producing Pseudomonas aeruginosa. In this study MBL-producing P. aeruginosa isolates identified in Norway (n ‫؍‬ 4) and Sweden (n ‫؍‬ 9) from 1999 to 2007 were characterized. Two international clonal complexes (CC), CC111 (n ‫؍‬ 8) and CC235 (n ‫؍‬ 2), previously associated with MBL-producing isolates, were dominant. CC111 isolates (ST111/229; serotype O12; bla VIM-2 ) included clonally related isolates identified in Skåne County, Sweden (n ‫؍‬ 6), and two isolates associated with importation from Greece and Denmark. In all CC111 isolates, bla VIM-2 was located in integron In59.2 or In59 variants. The two CC235 isolates (ST235/ST230; serotype O11; bla VIM-4 ) were imported from Greece and Cyprus, were possibly clonally related, and carried bla VIM-4 in two different integron structures. Three isolates imported from Ghana (ST233; serotype O6; bla VIM-2 ), Tunisia (ST654; serotype O11; bla VIM-2 ), and Thailand (ST260; serotype O6; bla IMP-14 ) were clonally unrelated. ST233 was part of a new CC (CC233) that included other MBL-producing isolates, while ST654 could also be part of a new CC associated with MBL producers. In the isolates imported from Ghana and Tunisia, bla VIM-2 was part of unusual integron structures lacking the 3 conserved segment and associated with transposons. The bla VIM gene was found to be located on the chromosome in all isolates. Known risk factors for acquisition of MBL were reported for all patients except one. The findings suggest that both import of successful international clones and local clonal expansion contribute to the emergence of MBL-producing P. aeruginosa in Scandinavia.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Molecular Epidemiology of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa Isolates from Norway and Sweden Shows Import of International Clones and Local Clonal Expansion

Samuelsen

Toleman

Sundsfjord

et al. 2010

Antimicrob Agents Chemother

153

120

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“…In particular, most Argentinean isolates harbored integron InPSG (11,13), which was also the most common integron platform among Italian isolates (13) and that found in isolates from Romania (9) and Austria (7). Moreover, sequence analysis showed that InPSG from the Argentinean isolates was inserted into a Tn5051-like transposon at the same res insertion site already described for the InPSG-harboring Italian isolates (11), suggesting a common origin for these genetic structures.…”

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confidence: 89%

Intercontinental Dissemination of IMP-13-Producing Pseudomonas aeruginosa Belonging in Sequence Type 621

et al. 2010

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“…This lineage belongs to the O12 serotype, which has been associated with multidrug resistance and expansion in hospitals for decades (1,11). It has been described in many European and Mediterranean countries, including France, Italy, Greece, the Netherlands, Spain and the Canary islands, Hungary, Scandinavia, Croatia, Austria, and Germany (6,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21) as well as in the United Kingdom (22,23). It has been frequently associated with VIM-2 or less commonly with VIM-4 or IMP-type MBLs but sometimes only with noncarbapenemase beta-lactamases, such as VEB-1 and OXA extendedspectrum beta-lactamases (24,25).…”

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confidence: 99%

High-Resolution Analysis by Whole-Genome Sequencing of an International Lineage (Sequence Type 111) of Pseudomonas aeruginosa Associated with Metallo-Carbapenemases in the United Kingdom

et al. 2015

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(1); one isolate had VIM-2 and IMP-18, and 7 carried no metallo-beta-lactamase (MBL) gene. Single nucleotide polymorphism analysis divided the isolates into distinct clusters; the NDM-1 isolate was an outlier, and the IMP isolates and 6/7 MBL-negative isolates clustered separately from the main set of 73 VIM-2 isolates. Within the VIM-2 set, there were at least 3 distinct clusters, including a tightly clustered set of isolates from 3 hospital laboratories consistent with an outbreak from a single introduction that was quickly brought under control and a much broader set dominated by isolates from a long-running outbreak in a London hospital likely seeded from an environmental source, requiring different control measures; isolates from 7 other hospital laboratories in London and southeast England were also included. Bayesian evolutionary analysis indicated that all the isolates shared a common ancestor dating back ϳ50 years (1960s), with the main VIM-2 set separating approximately 20 to 30 years ago. Accessory gene profiling revealed blocks of genes associated with particular clusters, with some having high similarity (>95%) to bacteriophage genes. WGS of widely found international lineages such as ST-111 provides the necessary resolution to inform epidemiological investigations and intervention policies. Concern over the increasing prevalence in hospitals of multiresistant bacteria, especially those that are resistant to carbapenem antibiotics, has prompted the use of typing methods that easily allow interlaboratory comparison of isolates. As a result, it has become clear that (i) high-risk clones of various Gram-negative bacteria, including Pseudomonas aeruginosa, can be found in many disparate locations, often with no obvious epidemiological links between the affected patients, and (ii) many of these clones are adept at acquiring various antibiotic resistance determinants (1, 2). These clones are commonly referred to as international lineages since they have been described in many countries across the globe. Epidemiological investigations of apparent outbreaks and interhospital spread of such lineages are confounded by the fact that they are so widely found; microbiological evidence of the same type must be considered alongside information about patient location and movement, for example, and patient location and movement information should be sufficiently robust to allow firm conclusions to be drawn. Greater resolution than that provided by conventional molecular typing methods, such as multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and variable-number tandem repeat (VNTR) analysis, is essential if we are to infer or discount potential transmission pathways with confidence. Understanding these pathways will be critical for precise public health interventions to contain these highrisk clones effectively. Whole-genome sequencing (WGS) can provide this resolution and has been used to investigate outbreaks of Klebsiella pneumoniae ST-258 producing K. pneumoniae carbapenemase (KPC) enz...

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First Organisms with Acquired Metallo-β-Lactamases (IMP-13, IMP-22, and VIM-2) Reported in Austria

Cited by 29 publications

References 17 publications

Molecular Epidemiology of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa Isolates from Norway and Sweden Shows Import of International Clones and Local Clonal Expansion

Molecular Epidemiology of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa Isolates from Norway and Sweden Shows Import of International Clones and Local Clonal Expansion

Intercontinental Dissemination of IMP-13-Producing Pseudomonas aeruginosa Belonging in Sequence Type 621

High-Resolution Analysis by Whole-Genome Sequencing of an International Lineage (Sequence Type 111) of Pseudomonas aeruginosa Associated with Metallo-Carbapenemases in the United Kingdom

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