First Pregnancies Carried to Term After Transfer of Vitrified Buffalo Embryos Entirely Produced in vitro

Neglia, Gianluca; Gasparrini, B.; Brienza, V. Caracciolo di; Palo, R. Di; Zicarelli, L.

doi:10.1023/b:verc.0000045414.65331.6a

Cited by 20 publications

(10 citation statements)

References 5 publications

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“…The results of Experiment 1 demonstrated that CTV is an efficient method to cryopreserve advanced IVP embryos in buffalo species, as indicated by the higher survival rates compared to those previously reported employing both the traditional straws (Neglia et al, 2004) and open pulled straw (De Rosa et al, 2006). Similar survival rates were reported for buffalo nuclear transfer HBL cryopreserved by CTV in an earlier work (Laowtammathron et al, 2005).…”

Section: Resultssupporting

confidence: 89%

“…The overall pregnancy rate here recorded was similar to that previously reported in another study after ET of vitrified embryos (Hufana-Duran et al, 2004). On the contrary, it was slightly lower than that we previously obtained in buffalo heifers in an earlier study, in which embryos were vitrified with 3.4 M glycerol and 4.6 M EG in French straws (Neglia et al, 2004).…”

Section: Pregnancysupporting

confidence: 63%

“…In Experiment 2, ovum pick-up was performed twice/week on 10 lactating pluriparous buffalo cows, over 10 sessions, as previously described (Neglia et al, 2004). The donors were under controlled nutrition, barn-housed, and the farm was in appropriate sanitary condition.…”

Section: Oocyte Source and In Vitro Maturationmentioning

confidence: 99%

“…Indeed, buffalo IVP embryos are very sensitive to cryopreservation, likely due to their high lipid content (Gasparrini, 2002). Nevertheless, they have been successfully cryopreserved by vitrification, as demonstrated by their survival following in vitro culture (Gasparrini et al, 2001) and development to term after embryo transfer (ET) (Hufana-Duran et al, 2004;Neglia et al, 2004). However, efficiency still needs to be improved for the diffusion of OPU-IVEP technologies in the field.…”

Section: Introductionmentioning

confidence: 97%

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Developmental Speed Affects the Cryotolerance ofIn VitroProduced Buffalo (Babalus Bubalis) Embryos

Boccia

Rosa

Attanasio

et al. 2013

Italian Journal of Animal Science

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The aim of this study was to evaluate whether the developmental speed affects the cryotolerance of in vitro produced buffalo embryos. In Experiment 1, abattoir-derived oocytes were in vitro matured, fertilized and cultured. The embryos produced by Say 7 of culture were vitrified at the tight morula (TM), early blastocyst (EBL), blastocyst (BL), expanded-blastocyst (XBL) and hatched-blastocyst (HBL) stage. The embryos were vitrified by cryotop in 16.5% ethylene glycol (EG) and 16.5% dimethyl sulfoxide (DMSO) and 0.5 M sucrose. Embryos were warmed in 0.25 M sucrose for 1 min and then in 0.15 M sucrose for 5 min and cultured in vitro for 24 h, to evaluate post-culture viability. In Experiment 2, ovum pick-up (OPU) was carried out on lactating buffaloes to produce embryos that were vitrified-warmed and transferred into synchronized recipients. The lowest (P<0.01) survival rates were recorded with TM (22.4%) and the highest (P<0.01) with HBL (84.5%), whereas intermediate results were observed with EBL, BL and XBL (54.5, 64.7, 67.9%, respectively). Pregnancy rate on both Days 25 and 45 was significantly higher (P<0.05) for faster (XBL and HBL) developing embryos (75.0% and 62.5%, respectively) than for the slower (TM, EBL and BL) developing counterparts (36.4% and 0, respectively). Interestingly, pregnancies to term were only recorded when HBL were transferred (20%). In conclusion, it was demonstrated that the chronology of development is a major factor affecting the cryotolerance of in vitro produced buffalo embryos.

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Section: Resultssupporting

confidence: 89%

Section: Pregnancysupporting

confidence: 63%

Section: Oocyte Source and In Vitro Maturationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Developmental Speed Affects the Cryotolerance ofIn VitroProduced Buffalo (Babalus Bubalis) Embryos

Boccia

Rosa

Attanasio

et al. 2013

Italian Journal of Animal Science

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“…Early attempts to produce buffalo embryos in vitro resulted in poor efficiency because the methods were extrapolated from cattle, without accounting for species‐specific differences. Although the efficiency of buffalo IVEP technology has improved, development to term following transfer of cryopreserved embryos is still poor (Neglia et al. 2004; Sá Filho et al.…”

Section: Introductionmentioning

confidence: 99%

Ion, Protein, Phospholipid and Energy Substrate Content of Oviduct Fluid During the Oestrous Cycle of Buffalo (Bubalus bubalis)

Vecchio¹,

Neglia²,

Palo³

et al. 2009

Reproduction in Domestic Animals

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The aim of this research was to analyse the composition of oviduct fluid (ODF) in buffalo cows at different oestrous cycle phases to fulfil the requirements of buffalo embryos in vitro. ODF was collected by chronic cannulation from three cows that were synchronized by administering a synthetic prostaglandin. Based on hormonal profiles, the pre-ovulatory, ovulatory, post-ovulatory and luteal phases of the oestrous cycle were defined. The volume of ODF produced (ml/24 h) was influenced by the oestrous cycle, with values (mean ± SE) around ovulation (1.0 ± 0.2) greater (p < 0.05) than in both the luteal (0.4 ± 0.1) and the post-ovulatory phases (0.5 ± 0.1), but not different from the intermediate values in the pre-ovulatory phase (0.8 ± 0.2). Among cycle phases, no differences were found in sodium, potassium, calcium and magnesium concentrations (130.0 ± 1.1, 5.1 ± 0.3, 2.8 ± 0.1 and 0.59 ± 0.04 mmol/l respectively). Interestingly, the chloride secretion (μm/24 h) was higher (p < 0.05) at ovulation (150.2 ± 16.5) than during both the luteal (73.7 ± 22.0) and the post-ovulatory phases (63.7 ± 11.2), with intermediate values in the pre-ovulatory phase (113.4 ± 23.5). Glucose concentration (mmol/l) was higher (p = 0.056) in the pre-ovulatory phase (0.06 ± 0.02) than in the luteal (0.02 ± 0.01) and post-ovulatory (0.02 ± 0.01) phases but not different from values in the ovulatory phase (0.04 ± 0.02). Concentrations of pyruvate and lactate among oestrous cycle phases were similar (0.08 ± 0.01 and 1.0 ± 0.1 mmol/l respectively). The total quantity of phospholipids (μmol/24 h) was greater (p < 0.05) at ovulation (0.21 ± 0.02) compared with the luteal, pre-ovulatory and post-ovulatory phases of the cycle (0.09 ± 0.02, 0.13 ± 0.02 and 0.09 ± 0.01 respectively). No differences were found in either the protein concentration (1.8 ± 0.3 mg/ml) or the quantity of proteins secreted in 24 h (1.8 ± 0.4 mg) among oestrous cycle phases. In conclusion, this study provides the first characterization of buffalo ODF during the oestrous cycle, showing species-specific differences that may be useful for developing suitable media for buffalo in vitro embryo production.

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Vitrification: Research in Animal Models

Vajta¹

2012

Practical Manual of in Vitro Fertilization

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First Pregnancies Carried to Term After Transfer of Vitrified Buffalo Embryos Entirely Produced in vitro

Cited by 20 publications

References 5 publications

Developmental Speed Affects the Cryotolerance ofIn VitroProduced Buffalo (Babalus Bubalis) Embryos

Developmental Speed Affects the Cryotolerance ofIn VitroProduced Buffalo (Babalus Bubalis) Embryos

Ion, Protein, Phospholipid and Energy Substrate Content of Oviduct Fluid During the Oestrous Cycle of Buffalo (Bubalus bubalis)

Vitrification: Research in Animal Models

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