First Prize: Isoprostane: Quantitation of Renal Ischemia and Reperfusion Injury After Renal Artery Clamping in an Animal Model

Wang, Zijun; Colli, Janet; Keel, Christopher; Bailey, Kayleen; Grossman, Leah; Majid, Dewan S. A.; Lee, Benjamin R.

doi:10.1089/end.2011.0188

Cited by 13 publications

(5 citation statements)

References 16 publications

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“…One of the approaches used to quantify oxidative stress in vivo consists of measuring the 8-isoprostane levels. In Wang et al 11 study, such levels significantly increased after 60 minutes of only ischaemia, while they did not change significantly after 15, 30 and 45 minutes. In that study, the authors compared the 8-isoprostane levels between two groups of rats subjected to 30 minutes of reperfusion after a period of 60 minutes of renal ischaemia, one of which was given 100 mg·kg -1 intravenous allopurinol, while the other group received no treatment.…”

Section: Effect Of Allopurinol On the Kidney Function Histology And mentioning

confidence: 80%

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

et al. 2014

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PURPOSE:To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM-1 and IL-18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury. METHODS:

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Section: Effect Of Allopurinol On the Kidney Function Histology And mentioning

confidence: 80%

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

et al. 2014

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“…Warm ischemia was identified as the “ultimate enemy” for partial nephrectomy [ 20 ], and several methods to ameliorate its negative aspects have been proposed [ 5 – 8 ]. Although mannitol is largely employed for this purpose [ 9 ], its effects for ischemia protection during partial nephrectomy were only recently investigated [ 10 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, recent studies have shown that the quality and quantity of remnant renal parenchyma is of great importance to predict renal function [ 4 ]. Thus, various methods have been employed to prevent damage to the remnant kidney parenchyma after prolonged warm renal ischemia [ 5 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Mannitol reduces nephron loss after warm renal ischemia in a porcine model

et al. 2018

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BackgroundMannitol has been employed to ameliorate renal warm ischemia damage during partial nephrectomy, however, there is limited scientific evidence to support the use of mannitol during partial nephrectomy. The objective of the present study was to investigate the glomerular number after renal warm ischemia, with and without the use of mannitol in a Pig Model.MethodsTwenty-four male pigs were assigned into three groups. Eight animals were allocated to the sham group that was subjected to laparoscopic dissection of the left renal hilum, without renal ischemia. Eight animals were allocated to the ischemia group that had the left renal hilum clamped for 30 min through laparoscopic access. Eight animals received mannitol (250 mg/kg) before the occlusion of renal hilum for 30 min. The kidneys were collected after the euthanasia of the pigs 21 days post surgery. The right kidney was utilized as a self-control for each animal. Serum creatinine, urea levels, the weight and volume of the kidneys were measured. Glomerular volumetric density, volume-weighted glomerular volume, and cortical volume were quantified through stereological methods and employed to determine the number of nephrons per kidney. Student’s t test and ANOVA were used for statistical analysis.ResultsIn the ischemia group, the left kidney recorded a reduction of 24.6% (290, 000 glomeruli) in the number of glomeruli in comparison to the right kidney. Kidneys subjected to ischemia also displayed decreased weight and volume in comparison to the sham and mannitol groups. No difference was observed between the left and right kidneys from the sham and mannitol groups. Further, no distinction in serum creatinine and urea among the groups was observed.ConclusionThe use of mannitol significantly reduces nephron loss during warm ischemia in pigs.

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“…Thus, here the applicability of the trans/cis-epoxy-PUFA ratio as oxidative stress marker cannot be evaluated based on comparison with IsoP levels. Even though IRI is linked to excess ROS formation [50,51] and an increase of oxidative damage derived IsoP following renal IRI has been observed in rat models [52,53], there are various factors influencing measured IsoP levels in this model. These include clamping time, since longer clamping seem to lead to higher IsoP levels [52], as well as the time point of sampling following reperfusion and the specimen analyzed, e.g.…”

Section: Correlation Of Isopmentioning

confidence: 92%

Formation of trans-epoxy fatty acids correlates with formation of isoprostanes and could serve as biomarker of oxidative stress

Rund

Heylmann

Seiwert

et al. 2019

Prostaglandins & Other Lipid Mediators

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In mammals, epoxy-polyunsaturated fatty acids (epoxy-PUFA) are enzymatically formed from naturally occurring all-cis PUFA by cytochrome P450 monooxygenases leading to the generation of cis-epoxy-PUFA (mixture of R,S-and S,R-enantiomers). In addition, also non-enzymatic chemical peroxidation gives rise to epoxy-PUFA leading to both, cis-and trans-epoxy-PUFA (mixture of R,R-and S,S-enantiomers). Here, we investigated for the first time trans-epoxy-PUFA and the trans/cis-epoxy-PUFA ratio as potential new biomarker of lipid peroxidation. Their formation was analyzed in correlation with the formation of isoprostanes (IsoP), which are commonly used as biomarkers of oxidative stress. Five oxidative stress models were investigated including incubations of three human cell lines as well as the in vivo model Caenorhabditis elegans with tert-butyl hydroperoxide (t-BOOH) and analysis of murine kidney tissue after renal ischemia reperfusion injury (IRI). A comprehensive set of IsoP and epoxy-PUFA derived from biologically relevant PUFA (ARA, EPA and DHA) was simultaneously quantified by LC-ESI(-)-MS/ MS. Following renal IRI only a moderate increase in the kidney levels of IsoP and no relevant change in the trans/cis-epoxy-PUFA ratio was observed. In all investigated cell lines (HCT-116, HepG2 and Caki-2) as well as C. elegans a dose dependent increase of both, IsoP and the trans/cis-epoxy-PUFA ratio in response to the applied t-BOOH was observed. The different cell lines showed a distinct time dependent pattern consistent for both classes of autoxidatively formed oxylipins. Clear and highly significant correlations of the trans/cis-epoxy-PUFA ratios with the IsoP levels were found in all investigated cell lines and C. elegans. Based on this, we suggest the trans/cis-epoxy-PUFA ratio as potential new biomarker of oxidative stress, which warrants further investigation.

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First Prize: Isoprostane: Quantitation of Renal Ischemia and Reperfusion Injury After Renal Artery Clamping in an Animal Model

Abstract: Our data have demonstrated that isoprostane levels are a potential real-time marker of renal ischemia and reperfusion injury. We also found allopurinol administration demonstrated a trend toward renoprotective abilities in the hilar occluded kidney.

Cited by 13 publications

References 16 publications

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Mannitol reduces nephron loss after warm renal ischemia in a porcine model

Formation of trans-epoxy fatty acids correlates with formation of isoprostanes and could serve as biomarker of oxidative stress

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