First record of epizootics in the ocola skipper,<i>Panoquina ocola</i>(Lepidopera: Hesperiidae), caused by<i>Isaria tenuipes</i>in flooded rice fields of Central Brazil

Mascarin, Gabriel Moura; Dunlap, Christopher A.; Barrigossi, J.A.F.; Quintela, E.D.; Noronha, Newton C.

doi:10.1111/jam.13390

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…For several years, Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) have helped study the epidemiology of many pathogens [8,[12][13][14][15] or the phylogenetic relationships between organisms [16]. The RAPD and AFLP methods have also been used to generate unique PCR products or amplicons in filamentous fungal species, or strains, of interest to be converted into species-or strain-specific Sequence-Characterized Amplified Region (SCAR) markers [17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Development of SCAR Markers for Genetic Authentication of Metarhizium acridum

Toriello,

Duarte-Escalante,

Frías-De-León

et al. 2024

JoF

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In this study, molecular typing using Randomly Amplified Polymorphic DNA (RAPD-PCR) was conducted on 16 original isolates of Metarhizium acridum obtained from locusts (Schistocerca piceifrons ssp. piceifrons.) in Mexico (MX). The analysis included reference strains of the genus Metarhizium sourced from various geographical regions. The isolates were identified by phenotypic (macro and micromorphology) and genotypic methods (RAPD-PCR and Amplified Fragment Length Polymorphisms (AFLP), through a multidimensional analysis of principal coordinates (PCoA) and a minimum spanning network (MST). Subsequently, Sequences-Characterized Amplified Region (SCAR) markers were developed for the molecular detection of M. acridum, these markers were chosen from polymorphic patterns obtained with 14 primers via RAPD-PCR. Phenotypic and genotypic characterization identified the MX isolates as M. acridum. Of all the polymorphic patterns obtained, only OPA04 and OPA05 were chosen, which presented species-specific bands for M. acridum, and further utilized to create SCAR markers through cloning and sequencing of the specific bands. The specificity of these two markers was confirmed via Southern hybridization. The SCAR markers (Ma-160OPA-05 and Ma-151OPA-04) exhibit remarkable sensitivity, detecting down to less than 0.1 ng, as well as high specificity, as evidenced by their inability to cross-amplify or generate amplification with DNAs from other strains of Metarhizium (as Metarhizium anisopliae) or different genera of entomopathogenic fungi (Cordyceps fumosorosea and Akanthomyces lecanii). These SCAR markers yield readily detectable results, showcasing high reproducibility. They serve as a valuable tool, especially in field applications.

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Section: Introductionmentioning

confidence: 99%

Development of SCAR Markers for Genetic Authentication of Metarhizium acridum

Toriello,

Duarte-Escalante,

Frías-De-León

et al. 2024

JoF

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“…Rice, Oryza sativa L. (Poaceae) is one of the most important staple foods, providing more than 20% of consumed calories worldwide. More than 60% of the world population depends on rice as their main food, especially in east and south Asia, the Middle East, the West Indies and Latin America (Fukagawa & Ziska, 2019; Mascarin et al, 2017; Sharif et al, 2014). In Iran, rice is one of the main foods and its consumption per capita is recorded at 40.6 kg in recent years (Helgi library, 2023).…”

Section: Introductionmentioning

confidence: 99%