First record of<i>Ceratocystis fimbriata</i>associated with shisham (<i>Dalbergia sissoo</i>) decline in Pakistan

Poussio, Gul Bahar; Kazmi, Munawar Raza; Akem, C.; Fateh, Faisal Sohail

doi:10.1071/dn10023

Cited by 22 publications

(24 citation statements)

References 3 publications

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“…was recently reported associated with P. cinerea and D. sissoo in Oman and Pakistan (Al Adawi et al 2009, Poussio et al 2010. The main objective of this study was to identify the species of Ceratocystis responsible for the disease associated with these native trees using morphological and DNA sequence comparisons.…”

mentioning

confidence: 94%

Ceratocystis manginecans associated with a serious wilt disease of two native legume trees in Oman and Pakistan

Adawi

Barnes

Khan

et al. 2013

Australasian Plant Pathol.

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A serious wilt disease has recently been found on Prosopis cineraria (Ghaf) in Oman and on Dalbergia sissoo (Shisham) in Pakistan. Disease symptoms on both these native, leguminous hosts include vascular discolouration and partial or complete wilt of affected trees. A species of Ceratocystis was consistently isolated from symptomatic material. Morphological comparisons and analyses of DNA sequence data of the ITS, β-tubulin, and EF 1-α gene regions showed that the Ceratocystis isolates obtained from both tree species represent C. manginecans. This is the same pathogen that is causing the devastating mango sudden decline disease in Oman and Pakistan. This is also the same pathogen that has been reported causing a wilting disease on Acacia mangium in Indonesia. Cross inoculation with C. manginecans isolates from P. cineraria, D. sissoo and mango showed that the fungus can cause disease on all three trees.

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confidence: 94%

Ceratocystis manginecans associated with a serious wilt disease of two native legume trees in Oman and Pakistan

Adawi

Barnes

Khan

et al. 2013

Australasian Plant Pathol.

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“…Both biotic and abiotic factors are probably involved in this problem. Many findings confirmed the association of Fusarium solani with shisham wilt and dieback in India (Shukla, ); Arif et al, ), and the involvement of Ceratocystis fimbriata alone or in combination with F. solani with shisham decline in Pakistan (Poussio, Kazmi, Akem, & Fateh, ). Another study by (Al Adawi et al, ) also confirmed that C. manginecans (which is now considered a synonym of C. fimbriata ) is associated with the wilt of shisham in Pakistan.…”

Section: Introductionmentioning

confidence: 67%

Biocontrol potential of Pseudomonas azotoformans, Serratia marcescens and Trichoderma virens against Fusarium wilt of Dalbergia sissoo

et al. 2020

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Wilt disease caused by Fusarium solani is a serious constraint to Dalbergia sissoo (shisham) plantations in northern India. In this study, the antagonistic potential of 40 bacterial isolates recovered from rhizophere soil of healthy shisham trees, and a well‐characterized Trichoderma species (Trichoderma virens) were tested for their possibility as biocontrol agents for F. solani. Two promising isolates (S1 and S15) were identified which inhibited pathogen growth, caused chitin degradation, produced siderophores and solubilized phosphate in vitro. Isolate S15 scored highest for hydrogen cyanide (HCN) production while isolate S1 was a non‐HCN producer. These two isolates were identified as Serratia marcescens (S1) and Pseudomonas azotoformans (S15) following sequence analysis of 16S rDNA. In dual culture assays, T. virens caused 80% inhibition of mycelial growth of the test fungus. The three selected antagonists when tested in planta in the glasshouse completely suppressed production of wilt symptoms on 12‐month‐old shisham plants. Further work is needed to ascertain the potential of these isolates to be used as biocontrol agents to manage shisham wilt under field conditions.

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“…; Poussio et al. ). In the present work, potentially pathogenic fungi were invariably isolated from every root and shoot sample of wilted D. sissoo plants, but not from healthy plants.…”

Section: Discussionmentioning

confidence: 96%

Molecular phylogeny and pathotyping of Fusarium solani: a causal agent of Dalbergia sissoo decline

et al. 2013

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Summary Sissoo (Dalbergia sissoo), commonly known as shisham, is amongst the finest woods of South Asia, but ‘wilt’ disease has caused a rapid decline in this species. The cause of the disease remains uncertain. The aim of this study is to identify the causal agent of the disease and characterize isolates made from diseased trees, based on genomic data and variations in virulence. Samples of infected roots, stems and the ooze exuded from infected trees were obtained from plants showing symptoms in different geographical regions of India for the isolation of microorganisms. Isolates were used to inoculate healthy plants. Based on the morphological characteristics, genus‐ and species‐specific PCR, and in silico analysis of 5.8S rDNA‐ITS regions, of the 38 fungal isolates, 24 and 14 were identified as Fusarium solani and Fusarium sp., respectively. In a pathotyping study, eighteen F. solani isolates, isolated from roots and stem parts of symptomatic plants, induced typical wilt symptoms when inoculated through soil and roots on D. sissoo seedlings of 1–15 months in age. The population of F. solani was the highest in infected roots and the lowest in parts of stems, gradually decreasing with height, and was isolated constantly up to approximately 40% height of the seedling. F. solani isolates used in inoculations were successfully re‐isolated from the rhizosphere, infected roots and wilted stems, as confirmed using isolate‐specific DNA fingerprints. Molecular phylogenies based on rDNA‐ITS sequences showed that the 38 isolates fell into 2 groups. Group I comprised of F. solani isolates from D. sissoo and F. solani sequences in the NCBI GenBank database, whereas group II included Fusarium isolates other than F. solani. These results are helpful in developing integrated control measures for this highly variable pathogen and to establish a base for future population studies.

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First record ofCeratocystis fimbriataassociated with shisham (Dalbergia sissoo) decline in Pakistan

Cited by 22 publications

References 3 publications

Ceratocystis manginecans associated with a serious wilt disease of two native legume trees in Oman and Pakistan

Ceratocystis manginecans associated with a serious wilt disease of two native legume trees in Oman and Pakistan

Biocontrol potential of Pseudomonas azotoformans, Serratia marcescens and Trichoderma virens against Fusarium wilt of Dalbergia sissoo

Molecular phylogeny and pathotyping of Fusarium solani: a causal agent of Dalbergia sissoo decline

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