We provide new data on the distribution of 14 long-legged fly species (Diptera, Brachycera, Dolichopodidae) belonging to 10 genera from the Fars Province, Southern Iran. The genera Diaphorus Meigen, 1824, Dolichopus Latreille, 1796, Hydrophorus Fallén, 1823, and Syntormon Loew, 1857 are recorded in the Fars Province for the first time. Dolichopus lairdi Olejnicek, Mohsen & Ouda, 1995 is newly added to the Iranian insect fauna. Four species, Asyndetus albifrons Loew, 1869, Asyndetus fallahzadehi Grichanov, 2019, Chrysotus suavis Loew, 1857, and Medetera pallipes (Zetterstedt, 1843) were the most abundant in the collected material. Available information on geographical distribution and short taxonomic comments for each species are also included.