First report about the screening, characterization, and fosmid library construction of Xanthomonas oryzae pv. oryzae strain with resistance to Fubianezuofeng

Yi, Chongfen; Chen, Jixiang; Hu, Deyu; Song, Baoan

doi:10.1016/j.pestbp.2020.104645

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…However, the misuse/overuse of antibiotics causes widespread antibiotic resistance, and these bacteria are insensitive to the currently available antibiotics. Recently, antibiotic-resistant X. oryzae strains were identified 2 of 13 in a number of studies [11][12][13]. Thus, the mechanisms underlying drug resistance have aroused the interest of researchers.…”

Section: Introductionmentioning

confidence: 99%

Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae

et al. 2021

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Xanthomonas oryzae severely impacts the yield and quality of rice. Antibiotics are the most common control measure for this pathogen; however, the overuse of antibiotics in past decades has caused bacterial resistance to these antibiotics. The agricultural context is of particular importance as antibiotic-resistant bacteria are prevalent, but the resistance mechanism largely remains unexplored. Herein, using gas chromatography–mass spectrometry (GC–MS), we demonstrated that zhongshengmycin-resistant X. oryzae (Xoo-Rzs) and zhongshengmycin-sensitive X. oryzae (Xoo-S) have distinct metabolic profiles. We found that the resistance to zhongshengmycin (ZS) in X. oryzae is related to increased fatty acid biosynthesis. This was demonstrated by measuring the Acetyl-CoA carboxylase (ACC) activity, the expression levels of enzyme genes involved in the fatty acid biosynthesis and degradation pathways, and adding exogenous materials, i.e., triclosan and fatty acids. Our work provides a basis for the subsequent control of the production of antibiotic-resistant strains of X. oryzae and the development of coping strategies.

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Section: Introductionmentioning

confidence: 99%

Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae

et al. 2021

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“…High capacity cosmid/fosmid-based genomic libraries can be produced using DNA from pure cultures of single bacterial species [16][17][18][19] or from complex metagenomic DNA samples such as those associated with soil [20,21] or gut [22,23] communities. Studies employing 25-40 kb genomic DNA inserts from single species typically involve the isolation, and banking, of between 1000 and 2500 clones before subsequently screening for the gain of targeted phenotypes and sequencing insert DNA [16][17][18][19]. As we intended to employ en masse antibiotic selection to validate our library, we bypassed the banking of individual clones and prepared a pooled library of tenfold higher coverage than that used in other similar studies -consisting of 2.5×10 4 c.f.u.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Validation of a random Vibrio parahaemolyticus genomic library by selection of quinolone resistance in a heterologous host

O’Boyle

Boyd

2022

Microbiology

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Vibrio parahaemolyticus is a shellfish-borne pathogen that is a highly prevalent causative agent of inflammatory gastroenteritis in humans. Genomic libraries have proven useful for the identification of novel gene functions in many bacterial species. In this study we prepared a library containing 40 kb fragments of randomly sheared V. parahaemolyticus genomic DNA and introduced this into Escherichia coli HB101 using a commercially available low copy cosmid system. In order to estimate coverage and suitability of the library and potentially identify novel antimicrobial resistance determinants, we screened for the acquisition of resistance to the fluoroquinolone norfloxacin – a phenotype exhibited by V. parahaemolyticus but not the heterologous E. coli host. Upon selection on solid medium containing norfloxacin, 0.52% of the library population was resistant, consistent with the selection of a single resistance locus. End-sequencing identified six distinct insert fragments. All clones displayed fourfold increased norfloxacin MIC compared with E. coli HB101 carrying an empty vector. The common locus contained within resistant clones included qnr, a previously described quinolone resistance gene. These results indicate that the library was unbiased, of sufficient coverage and that heterologous expression was possible. While we hope that this library proves useful for identifying the genetic determinants of complex phenotypes such as those related to virulence, not all norfloxacin resistance genes were detected in our screen. As such, we discuss the benefits and limitations of this approach for identifying the genetic basis of uncharacterized bacterial phenotypes.

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“…On the other, people blindly pursue bactericidal effect and abuse chemical bactericides for a long time, which causes the rapid development of drug resistance, shortened agent service life, and the increased difficulty and cost of plant disease control 15–17 . Currently, just a few agricultural bactericides such as bismerthiazol (BT), thiodiazole copper (TC), streptomycin, and zhongshengmycin are under long‐term use 18–22 . Therefore, there is an urgent requirement to discover and develop new agricultural bactericides to control plant pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Short pathways to highly active antimicrobial: structurally diverse polyamines derivatives from Amino‐Aldehyde condensation strategy

Ma,

Zhou,

Wang

et al. 2023

Pest Management Science

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BackgroundChemical fungicides are the mainstay of plant disease control in agricultural production, but there are a very limited number of drugs that can effectively control plant diseases. Two series of secondary amine derivatives were synthesized using the diamine skeleton combined with saturated aromatic and aliphatic aldehydes, and their antibacterial and antifungal activities against plant pathogens were determined. In addition, the antimicrobial mechanism of the highly active compound A26 was preliminarily examined against Xanthomonas oryzae (Xoo).ResultsCompound A26 exhibited the highest antibacterial potency among all the target compounds, with MIC values of 3.12, 3.12, and 12.5 μg/mL against Xoo, Xanthomonas axonopodis pv. Citri (Xac) and Pseudomonas sollamacearum (Ps), respectively. In addition, compound A26 had powerful curative and protective effects against Xoo at 200 μg/mL, and was better than the control agent Xinjunan. Preliminary mechanistic studies showed that compound A26 reduced the bacterial pathogenicity by targeting cell membranes and inhibiting the secretion of extracellular polysaccharides (EPS). Meanwhile, the toxicity of compound A26 to Human Embryonic Kidney 293 cells and Human Liver‐7702 was similar to that of Xinjunan, and it had the moderate toxicity according to the World Health Organization classification standard of oral exogenous toxicity with the LD50 of 245.47 mg/kg.ConclusionSecondary amines have efficient and broad‐spectrum antibacterial activity against plant pathogenic bacteria and are expected to be a new class of candidate compounds for antibacterial drugs.This article is protected by copyright. All rights reserved.

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First report about the screening, characterization, and fosmid library construction of Xanthomonas oryzae pv. oryzae strain with resistance to Fubianezuofeng

Cited by 10 publications

References 22 publications

Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae

Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae

Validation of a random Vibrio parahaemolyticus genomic library by selection of quinolone resistance in a heterologous host

Short pathways to highly active antimicrobial: structurally diverse polyamines derivatives from Amino‐Aldehyde condensation strategy

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