First Report, Characterization and Pathogenicity of Vibrio chagasii Isolated from Diseased Reared Larvae of Chilean Scallop, Argopecten purpuratus (Lamarck, 1819)

Urtubia, Rocío; Miranda, Claudio D.; Rodríguez, S.; Dubert, Javier; Barja, Juan L.; Rojas, Rodrigo

doi:10.3390/pathogens12020183

Cited by 11 publications

(5 citation statements)

References 54 publications

(84 reference statements)

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“…Likely of greater concern is the potential for oyster reefs to function as hot spots for PPM by condensing pathogens onto the benthos [202], where they can both infect reef inhabitants and be moved through the ecosystem via trophic transfer. Oyster reefs are homes to diverse assemblages of polychaetes, crustaceans, molluscs, and other animals [203][204][205][206] that can be infected by contacting or consuming the pathogens potentially found in the biodeposit samples investigated in this study [114,120,135]. Infected animals often die, but they can also spread pathogens to higher-level predators such as wading birds [207] and several commercially and recreationally important fishery species, including juvenile groupers, snappers and flounders, and adult drum and sheepshead [179,208].…”

Section: Discussionmentioning

confidence: 99%

Oyster Reefs Are Reservoirs for Potential Pathogens in a Highly Disturbed Subtropical Estuary

Sweat,

Busch,

Craig

et al. 2023

Environments

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Estuaries worldwide are grappling with deteriorating water quality and benthic conditions that coincide with the rising detection of pathogenic and potentially pathogenic microbes (PPM). Both indigenous PPM and those that enter estuaries through urban and agricultural runoff are funneled through suspension-feeding organisms and deposited onto the benthos, where they can be moved through food webs. This study explored PPM communities in the Indian River Lagoon system, a biodiverse but urbanized estuary in east central Florida (USA). PPM were surveyed in estuary water, at stormwater outfalls, and in biodeposits of a key suspension feeder, the eastern oyster Crassostrea virginica. A total of 52 microbial exact sequence variants, with per-sample relative abundances up to 61.4%, were identified as PPM. The biodeposits contained relatively more abundant and diverse PPM than the water samples. PPM community composition also differed between seasons and between biodeposits and water. The community differences were driven primarily by Vibrio and Pseudoalteromonas spp. This investigation provides evidence that, through biodeposition, oyster reefs in the IRL estuary are a reservoir for PPM, and it documents some taxa of concern that should be conclusively identified and investigated for their pathogenicity and potential to pervade food webs and fisheries.

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Section: Discussionmentioning

confidence: 99%

Oyster Reefs Are Reservoirs for Potential Pathogens in a Highly Disturbed Subtropical Estuary

Sweat,

Busch,

Craig

et al. 2023

Environments

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“…Initial studies on the colonization of pathogenic bacteria in larvae of different bivalves such as oysters, scallops and mussels were carried out mainly by microscopic histological observation [15,56,57]. However, this method does not allow the main pathogens to be distinguished from conventional microorganisms in larval tissues.…”

Section: Discussionmentioning

confidence: 99%

The Probiotic Bacillus hwajinpoensis Colonizes the Digestive System of Crassostrea gigas Larvae and Protects Them from Vibrio alginolyticus Infection

Zheng,

Huang,

Zhang

et al. 2023

Microorganisms

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The Pacific oyster Crassostrea gigas is one of the most important cultured marine species around the world. Production of Pacific oysters in China has depended primarily on hatchery produced seeds since 2016, with the successful introduction and development of triploid oysters. However, the seed supply of Pacific oysters is threatened by recurring mass mortality events in recent years. Vibriosis is the most commonly encountered disease associated with intensive oyster culture in hatcheries and nurseries. Vibrio alginolyticus and Bacillus hwajinpoensis were the two strains with pathogenic and probiotic effects, respectively, identified during the Pacific oyster larvae production. To monitor their colonization process in Pacific oyster larvae, green fluorescent protein (GFP) and red fluorescent protein (RFP) were labeled to the pathogenic V. alginolyticus and the probiotic B. hwajinpoensis stain, respectively. The pathogenic and probiotic effects of the two strains during the colonization process were then assessed. Stabile expression of GFP and RFP were observed in corresponding stains, and the capabilities of growth, biofilm formation and in vitro adhesion of GFP- and RFP- tagged stains were not significantly different from those of the wild-type strains. Usage of probiotics of 105 CFU/mL significantly inhibited the growth of pathogenic V. alginolyticus and reduced the mortality of D-sharped larvae. Both the pathogenic and probiotic strains employed a similar route to enter and colonize the oyster larvae, which indicates that competing with pathogens for binding and spreading sites were one of the mechanisms of B. hwajinpoensis to provide the probiotic effects to oyster larvae. In summary, employment of fluorescence-tagged pathogenic and probiotic strains simultaneously provides us with an excellent bioassay model to investigate the potential mechanisms of probiotics.

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“…Vibrio chagasii, previously isolated from various environments, including marine sediments, seawater, Artemia rotifers, turbot larvae, and sea bass [78][79][80], has been reported as a bacterium involved in oyster, scallop, and mussel diseases [81][82][83]. More recently, it was held responsible for the massive mortality of Argopecten purpuratus scallop larvae during vibriosis outbreaks in commercial scallop larvae hatcheries in Northern Chile [84]. Among the diseases caused by Vibrio spp., larval vibriosis can seriously threaten both the fishing industry and the marine ecosystem, leading to high mortality of the organisms [85,86].…”

Section: Discussionmentioning

confidence: 99%

Screening of Undaria pinnatifida (Laminariales, Phaeophyceae) Lipidic Extract as a New Potential Source of Antibacterial and Antioxidant Compounds

Stabili,

Acquaviva,

Cecere

et al. 2023

JMSE

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The lipidic extract of Undaria pinnatifida, one of the worst invasive species, was investigated for its potential exploitation in biotechnological applications. The antimicrobial activity of the lipidic extract in three different portions (blade, sporophyll, and holdfast) was assessed by using the Kirby–Bauer method, while the antioxidant activity was evaluated by the TEAC, ORAC, and Folin–Ciocalteu assays. NMR spectroscopy and thin-layer chromatography were employed for the chemical characterization. The extracts showed antibacterial activity against several of the tested Vibrio species: V. aestuarinus, V. fischeri, V. furnisii, V. inusitatus, V. litoralis, and V. mediterranei, including some pathogens for farmed fish. Intriguing antioxidant activity was recorded, with the highest value in the blade (126.907 ± 28.993 mmol Trolox equivalent/g TEAC). Free, saturated, unsaturated, and polyunsaturated fatty acids were highlighted by 1D and 2D NMR spectroscopy. The presence of ω-3 and ω-6 PUFAs indicates the importance of this algal species in the food industry. We suggest the employment of U. pinnatifida as source of new and safer therapeutic agents to control fish and shellfish diseases due to vibriosis, as well as a source of natural antioxidants that are useful for human health, considering the growing interest in the development of strategies for invasive seaweed control.

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First Report, Characterization and Pathogenicity of Vibrio chagasii Isolated from Diseased Reared Larvae of Chilean Scallop, Argopecten purpuratus (Lamarck, 1819)

Cited by 11 publications

References 54 publications

Oyster Reefs Are Reservoirs for Potential Pathogens in a Highly Disturbed Subtropical Estuary

Oyster Reefs Are Reservoirs for Potential Pathogens in a Highly Disturbed Subtropical Estuary

The Probiotic Bacillus hwajinpoensis Colonizes the Digestive System of Crassostrea gigas Larvae and Protects Them from Vibrio alginolyticus Infection

Screening of Undaria pinnatifida (Laminariales, Phaeophyceae) Lipidic Extract as a New Potential Source of Antibacterial and Antioxidant Compounds

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