First Report of a Lipopeptide Biosurfactant from Thermophilic Bacterium Aneurinibacillus thermoaerophilus MK01 Newly Isolated from Municipal Landfill Site

Sharafi, Hakimeh; Abdoli, Mahya; Hajfarajollah, Hamidreza; Samie, Nima; Alidoust, Leila; Abbasi, Habib; Fooladi, Jamshid; Zahiri, Hossein Shahbani; Noghabi, Kambiz Akbari

doi:10.1007/s12010-014-0928-9

Cited by 29 publications

(24 citation statements)

References 28 publications

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“…Lipopeptide produced by MSA31 was found to be thermostable as revealed by DSC analysis. Similar thermostable lipopeptide were produced by Nocardiopsis sp ., Brevibacterium aureum , and Aneurinibacillus thermoaerophilus MK01 (Gandhimathi et al, 2009; Kiran et al, 2010b; Sharafi et al, 2014). …”

Section: Resultsmentioning

confidence: 99%

Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

et al. 2017

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Biosurfactants are smart biomolecules which have wide spread application in medicines, processed foods, cosmetics as well as in bioremediation. In food industry, biosurfactants are used as emulsion stabilizing agents, antiadhesives, and antimicrobial/antibiofilm agents. Nowadays biosurfactant demands in industries has increased tremendously and therefore new bacterial strains are being explored for large scale production of biosurfactants. In this study, an actinobacterial strain MSA31 was isolated from a marine sponge Fasciospongia cavernosa which showed high activity in biosurfactant screening assays such as drop collapsing, oil displacement, lipase and emulsification. Lipopeptide produced by MSA31 was found to be thermostable which was evident in differential scanning calorimetry analysis. The spectral data obtained in the Fourier transform infrared spectroscopy showed the presence of aliphatic groups combined with peptide moiety which is a characteristic feature of lipopeptides. The stability index of lipopeptide MSA31 revealed “halo-alkali and thermal tolerant biosurfactant” which can be used in the food industry. Microtiter plate assay showed 125 μg/ml of lipopeptide was effective in reducing the biofilm formation activity of pathogenic multidrug resistant Staphylococcus aureus. The confocal laser scanning microscopic images provided further evidences that lipopeptide MSA31 was an effective antibiofilm agent. The antioxidant activity of lipopeptide MSA31 may be due to the presence of unsaturated fatty acid present in the molecule. The brine shrimp cytotoxicity assay showed lipopeptide MSA31 was non-toxic and can be used as food additives. Incorporation of lipopeptide MSA31 in muffin showed improved organoleptic qualities compared to positive and negative control. This study provides a valuable input for this lipopeptide to be used in food industry as an effective emulsifier, with good antioxidant activity and as a protective agent against S. aureus.

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Section: Resultsmentioning

confidence: 99%

Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

et al. 2017

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“…From the biological, biochemical, and structural point of view, the most biosurfactants are complex molecules comprising different structures. Here, they are represented in the following groups: (1) glycolipids, (2) lipopeptides and lipoproteins, (3) polysaccharide protein complex, (4) fatty acids, (5) phospholipids, and (6) polymeric biosurfactants . Other combination of these groups like glycolipopeptide and lipopolysaccharide also exist.…”

Section: Introductionmentioning

confidence: 99%

Biosurfactants from probiotic bacteria: A review

Hajfarajollah

Eslami

Mokhtarani

et al. 2018

Biotech and App Biochem

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Among microorganisms, bacteria are the main group of biosurfactant-producing organisms. Different types of bacteria including Pseudomonas sp., Acinetobacter sp., Bacillus sp., and Arthrobacter sp. are among the most commonly studied bacteria in the realm of scientific research. However, due to the pathogenic nature of the producing organisms, the application of these compounds is restricted, therefore, not suitable for use in food-related industries. Given that probiotic bacteria impact human health, applying probiotics as nonpathogenic and safe organisms have gained much attention for the production of biosurfactants in recent years. Most biosurfactants obtained from probiotic bacteria are related to a number of lactic acid bacteria (LAB). These types of biosurfactants are classified based on their structures as protein-carbohydrate complexes, lipids, or fatty acids. The present paper seeks to provide comprehensive and useful information about the production of various kinds of biosurfactants by different probiotic bacteria. In addition, we have extensively reviewed their potential for possible future applications.

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“…For Bacillus brevis, this was 71.89 ± 0.56% with crude oil (Mouafi et al, 2016). The stability of MSI 54 was higher than that reported Sharafi et al, 2014;Jha et al, 2016). The MSI 54 lipopeptide effectively remediated heavy metals such as mercury, lead, cadmium, and manganese at 2.0 × CMC.…”

Section: Discussionmentioning

confidence: 87%

“…Stability studies showed that the activity of the MSI 54 biosurfactant was not affected at higher temperature, pH, and salt concentrations, suggesting its environmental applications such as heavy metal remediation and metal recovery from the complex using ultrafiltration (Ferella et al, 2007). Several lipopeptide biosurfactants from marine sponge-associated bacteria have been reported Kiran et al, 2010bKiran et al, , 2017Sharafi et al, 2014). However, the MSI 54 lipopeptide biosurfactant seems to be unique based on GC-MS analysis; its stability and emulsification index were found to be higher than those reported.…”

Section: Discussionmentioning

confidence: 89%

Revealing the Efficacy of Thermostable Biosurfactant in Heavy Metal Bioremediation and Surface Treatment in Vegetables

et al. 2020

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Biosurfactants are amphiphilic molecules which showed application in the food, medical, and cosmetics industries and in bioremediation. In this study, a marine spongeassociated bacteria (MSI 54) was identified as a biosurfactant producer which showed high emulsification and surface tension-reducing property. The isolate MSI 54 was identified as Bacillus sp. and the biosurfactant was chemically characterized as a lipopeptide analog based on the spectral data including Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The MSI 54 lipopeptide biosurfactant was an anionic molecule which showed high affinity toward cationic heavy metals including Pb, Hg, Mn, and Cd. The heavy metal bioremediation efficacy of the biosurfactant was evaluated using atomic absorption spectroscopy, scanning electron microscopy/energy-dispersive X-ray spectroscopy, and high-resolution transmission electron microscopy analysis. When MSI 54 lipopeptide biosurfactant was added to heavy metals, this resulted in a white co-precipitate of the metal-biosurfactant complex. The heavy metal remediation efficacy of the biosurfactant at a 2.0 × critical micelle concentration (CMC) showed removal of 75.5% Hg, 97.73% Pb, 89.5% Mn, and 99.93% Cd, respectively, in 1,000 ppm of the respective metal solution. The surface treatment of farm fresh cabbage, carrot, and lettuce with 2.0 × CMC of the lipopeptide showed effective removal of the surface heavy metal contaminants.

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First Report of a Lipopeptide Biosurfactant from Thermophilic Bacterium Aneurinibacillus thermoaerophilus MK01 Newly Isolated from Municipal Landfill Site

Cited by 29 publications

References 28 publications

Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

Biosurfactants from probiotic bacteria: A review

Revealing the Efficacy of Thermostable Biosurfactant in Heavy Metal Bioremediation and Surface Treatment in Vegetables

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