First report of a livestock‐associated methicillin‐resistant
            <i>Staphylococcus aureus</i>
            ST126 harbouring the
            <i>mecC</i>
            variant in Brazil

Silva, José Givanildo da; Araújo, Wydemberg J.; Leite, Elma Lima; Dias, Larissa Maranhão; Vasconcelos, Priscylla Carvalho; Silva, Núbia Michelle Vieira; Oliveira, Raylson Pereira de; Sena, Marcelo M.; Oliveira, Celso José Bruno de; Mota, Rinaldo Aparecido

doi:10.1111/tbed.13771

Cited by 39 publications

(8 citation statements)

References 53 publications

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“…We identify ST126 among the MSSA isolates, but not among the mecC MRSA detected. Our report, in addition to the study by Silva and collaborators [18], may indicate a more widely spread of mecC among livestockassociated S. aureus in Brazil. Future studies are needed to investigate the extent of this change in S. aureus epidemiology, the animal management, and the potential dissemination of mecC strains in humans, in order to prevent public health and economic impacts.…”

supporting

confidence: 76%

“…Interestingly, the MRSA ST746 is genetically similar to ST97, varying in the aroE gene. Only recently, Silva and co-workers identified an MRSA ST126 harboring the mecC gene [18]. We identify ST126 among the MSSA isolates, but not among the mecC MRSA detected.…”

contrasting

confidence: 44%

“…This is the first report of mecC-positive LA-MRSA isolates in Southeastern Brazil. Just recently, Silva and co-workers reported the occurrence of a LA-MRSA ST126 harboring the mecC variant in the North of Brazil [18]. MRSA harboring mecC has already been identified in milk samples from animal origin in previous studies elsewhere [7,12,19,20].…”

mentioning

confidence: 95%

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First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil

et al. 2020

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MRSA infection and colonization have been reported in both companion and food-chain animals, highlighting MRSA as an important veterinary and zoonotic pathogen. Another mec allele, the mecC gene, also confers beta-lactam resistance in Staphylococcus aureus and shows 69% nucleotide identity to mecA. The main aim of this study was to investigate the genotypic and clonal profile of methicillin-resistant S. aureus (MRSA) from cows with mastitis in dairy herds. Thirty-five samples suggestive of bovine subclinical mastitis were evaluated, and S. aureus were detected in all of them using both phenotypic and molecular approaches. According to the multilocus sequence typing (MLST), the S. aureus isolates were assigned in five different STs, 21 (60%) showed ST 742, 6 (17%) ST97, 4 (11%) ST1, 2 (6%) ST30, and 2 (6%) ST126. The presence of mecA was not observed in any of these isolates whereas mecC was detected in nine of them (9/35; 26%). The Panton-Valentine leukocidin (PVL) genes were detected in a total of 4 isolates. Among the 35 isolates analyzed, 26 showed resistance to penicillin. Changes in the S. aureus epidemiology due to the detection of MRSA in milk samples from cows presenting with bovine subclinical mastitis may have consequences for public health in Brazil, challenging the empirical therapy and animal management, with potential medical and social outcomes. To the best of our knowledge, this is the first report describing mecC MRSA in Southeastern Brazil.

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supporting

confidence: 76%

contrasting

confidence: 44%

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First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil

et al. 2020

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“…The antibiotic susceptibility pattern of strain SA10610, as reported for most mecC MRSA isolates, 5 , 15 , 18 , 29 , 32 is characterized by susceptibility to the majority of all non-β-lactam antibiotics. 35 In addition, all the genomes tested in this analysis, except from the isolate isolated in Brazil, carry the same SCC mec and have very similar horizontally transmissible accessory genomes.…”

Section: Discussionmentioning

confidence: 90%

“…The following genomes were used for WGS analysis comparison: mec A LGA251 5 (accession: FR821779); M10/0061 7 (accession: FR823292); Patient A 20 (accession: ERR084771); Cow A 20 (accession: ERR144792); Patient B 20 (accession: ERR144788); Sheep B 20 (accession: ERR144749); OFVD (accession: OFVD01000000); OFUF (accession: OFUF01000000); mecC MRSA isolated in Spain 29 (accession: ERR403511); mecC MRSA isolated in Brazil 15 (GCA_009763195.1); mecC MRSA isolated in New Zealand 29 (ERR5417136); mecC MRSA isolated in England 29 (ERR3595448); and mecC MRSA isolated in Australia 18 (accession: LUFG00000000). WGS of strain SA10610 was deposited in the pubMLST database under the ID 34000.…”

Section: Methodsmentioning

confidence: 99%

mecCMRSA in Israel—genomic analysis, prevalence and global perspective

Baum

Anuka

Treygerman

et al. 2022

JAC-Antimicrobial Resistance

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Background MRSA is a major global healthcare problem. In 2011, a new mec variant designated mecC was described, presenting partial identity at the DNA level, thus undetectable by routine mecA PCR. Objectives Until now, no reliable information regarding mecC MRSA prevalence was available in Israel. In this study, to the best of our knowledge, we describe the first case of mecC MRSA in Israel, with focus on genomic analysis and global context. Methods The mecC MRSA isolate was analysed by WGS with focus on phylogeny, global contextualization, virulence and resistance genes. The strain was characterized by antibiotic susceptibility testing, spa typing and presence of mecA/C and pvl genes. Results An MRSA strain (SA10610), isolated from a urine sample of an 83-year old patient, was found negative for the mecA and pvl genes. The MLST and spa type were ST130 and t1736, respectively. SA10610 presented resistance to oxacillin, penicillin and cefoxitin, and susceptibility to all non-β-lactam agents tested. Phylogenetic comparison with a global dataset of 586 mecC MRSA genomes revealed substantial genomic divergence. The nearest genomic relatives were human and animal isolates from Denmark. A screen of 12 761 S. aureus isolates collected during 2011–18 in Israel indicated this is the only mecC-positive strain. Conclusions A high degree of genetic variability was found between the SA10610 strain and previously sequenced mecC MRSA isolated worldwide. The genomic and phylogenetic analysis suggest that mecC MRSA isolates have evolved independently rather than from a common ancestor.

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Diversity and emergence of multi-resistant Staphylococcus spp. isolated from subclinical mastitis in cows in of the state of Piauí, Brazil

et al. 2022

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First report of a livestock‐associated methicillin‐resistant Staphylococcus aureus ST126 harbouring the mecC variant in Brazil

Cited by 39 publications

References 53 publications

First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil

First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil

mecCMRSA in Israel—genomic analysis, prevalence and global perspective

Diversity and emergence of multi-resistant Staphylococcus spp. isolated from subclinical mastitis in cows in of the state of Piauí, Brazil

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