First report of a phytoplasma associated with bell pepper big bud disease in Iran

Faghihi, Mohammad Mehdi; Taghavi, Seyed Mohsen; Safaei, Ali; Siampour, Majid; Najafabadi, Seyed Saeid Modarres

doi:10.5197/j.2044-0588.2016.033.015

Cited by 13 publications

(7 citation statements)

References 6 publications

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“…Besides well‐known fungal and virus pathogens, recently, phytoplasma has become increasingly important as a serious threat in many different crops around the world (Junqueira, Bedendo, & Pascholati, ; Margaria et al., ), and the pepper crop is not the exception. Phytoplasma associated with C. annuum diseases have been reported worldwide, including bell pepper leaf size reduction (16SrIII) in Bolivia, bell and chilli pepper big bud in Iran and the United States, respectively (16SrII and 16SrVI) and pepper witches’ broom (16SrI) in China and México (Arocha et al., ; Faghihi, Taghavi, Safaei, Siampour, & Najafabadi, ; Randall, Bosland, & Hanson, ; Santos‐Cervantes, Chávez‐Medina, Mendez‐Lozano, & Leyva‐Lopez, ; Zheng‐Nan, Zhang, Song, & Wu, ). Of late, Mauricio‐Castillo, Salas‐Muñoz, Velásquez‐Valle, Ambríz‐Granados, and Reveles‐Torres () reported the presence of “ Candidatus Phytoplasma trifolii” (16SrVI) in Zacatecas, México.…”

Section: Introductionmentioning

confidence: 99%

Candidatus Phytoplasma trifolii (16SrVI) infection modifies the polyphenols concentration in pepper (Capsicum annuum) plant tissues

Reveles‐Torres

Velásquez‐Valle

Salas-Muñoz

et al. 2018

Journal of Phytopathology

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Of late, the presence of Candidatus Phytoplasma trifolii was reported as a serious threat to the pepper crop in Zacatecas, México; therefore, asymptomatic and symptomatic pepper plants were collected from a commercial field among three samplings after the fruit set stage was reached. Total DNA was extracted using the CTAB‐based method and tested for phytoplasma using a nested PCR assay, followed by a BLAST, and restriction fragment length polymorphism (RFLP) analysis of 16S rDNA sequences, which confirmed the presence of phytoplasma group 16SrVI, “Candidatus Phytoplasma trifolii” in the symptomatic plants. As the metabolic pathways of pathogen‐infected plants tend to change, resulting in a biochemical differentiation with the noninfected plants, the polyphenolic compound concentrations were quantified from the vegetative tissues (root, stem, leaves and developed fruit/big bud) and were analysed based on a principal component analysis (PCA). Results revealed that, in general, plants tend to a progressive increase in total phenols, flavonoids, condensed tannins and anthocyanins related to the plants exposure to “Ca. P. trifolii” infection, and PCA demonstrated that almost 90% of the observed variance was explained by the first two components. Hence, the phenolic content of the plants increases as a response of the defence mechanism, which reflects its condition and resistance.

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Section: Introductionmentioning

confidence: 99%

Candidatus Phytoplasma trifolii (16SrVI) infection modifies the polyphenols concentration in pepper (Capsicum annuum) plant tissues

Reveles‐Torres

Velásquez‐Valle

Salas-Muñoz

et al. 2018

Journal of Phytopathology

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“…The 16SrII-D subgroup strains were also indicated to cause diseases in other plants in Iran, including Capsicum annuum, Zinnia elegans, and Artemisia sieberi in the south of the country (Hormozgan province, Faghihi et al, 2016;Hemmati & Nikooei, 2017,…”

Section: Discussionmentioning

confidence: 99%

“…The 16SrII‐D subgroup strains were also indicated to cause diseases in other plants in Iran, including Capsicum annuum , Zinnia elegans , and Artemisia sieberi in the south of the country (Hormozgan province, Faghihi et al, 2016; Hemmati & Nikooei, 2017, 2019), Albizia lebbeck , and Pelargonium hortorum in the southern part of Kerman province (Amirmijani et al, 2020; Salari & Azadvar, 2019). In addition, the plants Vicia faba and Punica granatum in Fars province (Iran's southwest) (Salehi, Esmaeilzadeh‐Hosseini, et al, 2016; Salehi, Rasoulpour, & Izadpanah, 2016), as well as Petroselinum crispum and Heliopsis helianthoides in the country's central region (Yazd province) (Babaei et al, 2022; Salehi, Esmailzadeh Hosseini, et al, 2016) have been reported to be infected by the same phytoplasma subgroup strains.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Movahedi‐Parizi

Hosseinipour

Azadvar

et al. 2022

Annals of Applied Biology

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Given the potential for urban green spaces to provide fresh and healthy environments for humans, exploring the issues that threaten plants in these places is crucial.Phytoplasma-related symptoms were encountered on some plants in urban green spaces in the province of Kerman, southeastern Iran, between 2017 and 2019. Affected periwinkles and petunias exhibited phytoplasma disease symptoms, including virescence, phyllody, and witches'-broom. However, ball or disc-like shoot proliferation symptoms were noticed on the trunks and branches of pine trees. PCR was performed with phytoplasma-detecting universal primers, targetting and amplifying the 16S rRNA gene, and determining whether phytoplasmas are implicated in the symptomatic plants. The infection of the symptomatic plants was confirmed using nested-PCR amplification of expected DNA sizes for phytoplasmas. No product, however, was amplified from sampled symptomless plants. The sequencing of nested-PCR products was performed to obtain sequences encasing the standard F2nR2 fragments. The resulted sequences were submitted to iPhyClassifier, the universal phytoplasma classification platform, for the taxonomic assignment of the found phytoplasmas compared with previously identified 'Candidatus Phytoplasma' species, groups, and subgroups. The results revealed that phytoplasma strains related to the species 'Ca. P. trifolii' (16SrVI-A subgroup) infect periwinkles and pines. However, strains from the species 'Ca. P. aurantifolia' (16SrII-D subgroup) and 'Ca. P. phoenicium' (16SrIX-C subgroup) were found in petunias and periwinkles, respectively. To the best of our knowledge, phytoplasmas from the 16SrVI-A and 16SrII-D subgroups are the first reported to infect these plants in Kerman province, while a related strain from the subgroup 16SrIX-C is the first recorded to infect periwinkles in Iran and the second in the world.

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“…P. solani') in pepper with big bud symptoms. In addition, Harling et al (2009) and Faghihi et al (2016) reported the'Ca. P. aurantiolia' (16SrII group) in pepper plants exhibiting symptoms such as small-leaved, phyllody (virescence), big bud, and rosetting from Indonesia and Iran.…”

Section: Cladistic Analysis Of Phytoplasma Igdır I3 Isolatementioning

confidence: 99%

Türkiye’nin Iğdır İli’nde sararma ve çalımsı görünüm gösteren biber bitkilerinde ‘Candidatus phytoplasma trifolii’nin varlığı

Güller

Usta²,

KORKMAZ³

et al. 2022

Ege Üniversitesi Ziraat Fakültesi Dergisi

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Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

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First report of a phytoplasma associated with bell pepper big bud disease in Iran

Cited by 13 publications

References 6 publications

Candidatus Phytoplasma trifolii (16SrVI) infection modifies the polyphenols concentration in pepper (Capsicum annuum) plant tissues

Candidatus Phytoplasma trifolii (16SrVI) infection modifies the polyphenols concentration in pepper (Capsicum annuum) plant tissues

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Türkiye’nin Iğdır İli’nde sararma ve çalımsı görünüm gösteren biber bitkilerinde ‘Candidatus phytoplasma trifolii’nin varlığı

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