First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan

Matsushita, Yosuke; Kanda, Ayami; Usugi, Tomio; Tsuda, Shinya

doi:10.1007/s10327-008-0076-6

Cited by 46 publications

(28 citation statements)

References 14 publications

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“…It was first reported from Canada (Singh et al 1999) on greenhouse tomato (Solanum lycopersicum, formerly Lycopersicon esculentum) plants exhibiting chlorotic leaves and dwarfing symptoms. Since then, it has been reported on tomatoes from the Netherlands (Verhoeven et al 2004), Japan (Matsushita et al 2008), France (acc. # EU729744) and the USA (Ling et al 2009).…”

Section: Introductionmentioning

confidence: 97%

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Molecular and biological characterization of a severe isolate of Tomato chlorotic dwarf viroid containing a novel terminal right (TR) domain sequence

Singh

Dilworth

et al. 2009

Eur J Plant Pathol

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Tomato chlorotic dwarf viroid (TCDVd) manually inoculated to transgenic (cv.'Desiree') potato plants containing antimicrobial cationic peptides failed to develop symptoms in above ground plant parts, but infected tubers were symptomatic. Plants from the infected tubers (second generation plants) emerged as either severely stunted (bushy stunt isolate, BSI) or tall and symptomless. Molecular characterization of BSI isolates showed TCDVd sequence variants 95 to 98% identical to TCDVd sequences from the database, while a viroid variant identical to TCDVd type isolate (acc # AF162131) was cloned from symptomless plants. The TCDVd BSI variants had novel U165C, GU177-178AA, and UCAC181-184CUUU nucleotide substitutions in the terminal right (T R ) domain of the viroid molecule. The cloned viroid cDNAs of the BSI were infectious to experimental (cv. 'Sheyenne') tomato plants causing stunted plants with profuse auxiliary shoots. Visual evaluation of the susceptibility of the BSI to 18 potato and 21 tomato cultivars revealed severe symptoms in most cultivars of both species. The progeny variants accumulating in each potato and tomato cultivar exhibited the same novel T R domain in most cultivars, with only a slight variation in a few. The severity of the stunting symptoms induced by TCDVd from BSI isolates in both potato and tomato cultivars has not been noted previously with other TCDVd isolates and, as such, it is proposed that this new isolate be recognized as a distinct genotype. Emergence of this type of sequence variant in commercial fields or commercial tomato greenhouses could potentially cause relevant losses in both crops.

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Section: Introductionmentioning

confidence: 97%

“…TCDVd can infect plants in the families of Apocynaceae, Compositae, Solanaceae and Verbenaceae (Matsushita et al 2009). Thus, TCDVd is well-adapted to survive in greenhouse growing conditions and can spread rapidly as evidenced by an increase of over 3,000 symptomatic tomato plants within a 6-month period (Matsushita et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular and biological characterization of a severe isolate of Tomato chlorotic dwarf viroid containing a novel terminal right (TR) domain sequence

Singh

Dilworth

et al. 2009

Eur J Plant Pathol

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“…PSTVd has also been detected in avocado (Persea americana) (Querci et al 1995), pepino (S. muricatum) (Puchta et al 1990;Shamloul et al 1997) Infected seed and symptomless carriers of PSTVd are suspected to be responsible for recent outbreaks in tomatoes. Fortunately, PSTVd has never occurred in Japan, although an outbreak of tomato disease caused by the closely related Tomato chlorotic dwarf viroid (TCDVd) was reported recently (Matsushita et al 2008), and there is concern over potential invasion of PSTVd from abroad. No natural resistance against PSTVd has been reported.…”

Section: Introductionmentioning

confidence: 99%

Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA

Hataya

2009

J Gen Plant Pathol

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An accurate and reliable system to detect Potato spindle tuber viroid (PSTVd) was developed based on duplex reverse transcription-polymerase chain reaction (RT-PCR) in combination with a plant mRNA internal control to evaluate the quality of RNA extracted from a dormant potato tuber and the effectiveness of the RT-PCR. In addition, a non-infectious RNA based on the PSTVd sequence was generated in vitro as a readily available positive control without the possibility of escape; this RNA was distinguishable from true positives in PSTVd infection. This detection system will be useful in plant quarantine and phytosanitary certification applications.

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“…Reverse transcription-polymerase chain reaction (RT-PCR) analysis, using methods reported previously (Matsushita et al 2008;Verhoeven et al 2009), was negative for three Solanaceae-infecting viroid species: pepper chat fruit viroid, Potato spindle tuber viroid, and Tomato chlorotic dwarf viroid. Electron microscopic observations of sap from the diseased leaves revealed the presence of a large number of rod-shaped virus-like particles with a length of ca.…”

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confidence: 99%

Characterization of Rehmannia mosaic virus isolated from chili pepper (Capsicum annuum) in Japan

et al. 2011

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In 2010, severe necrotic mosaic disease and fruit distortion were observed on greenhouse-grown chili pepper (Capsicum annuum cv. Fushimi-amanaga) plants in Kyoto Prefecture, Japan. Electron microscopic imaging and genomic RNA sequencing indicated that the virus responsible was a new isolate of Rehmannia mosaic virus (ReMV), which had not been previously reported in Japan. Although ReMV systemically infected many Solanaceae species, including chili pepper and tomato (Solanum lycopersicum), tobamovirus-resistance genes from species of Capsicum (L 1a , L 2 , L 3 , and L 4 ) and tomato (Tm-1, Tm-2, and Tm-2 a ) conferred resistance against ReMV.

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First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan

Cited by 46 publications

References 14 publications

Molecular and biological characterization of a severe isolate of Tomato chlorotic dwarf viroid containing a novel terminal right (TR) domain sequence

Molecular and biological characterization of a severe isolate of Tomato chlorotic dwarf viroid containing a novel terminal right (TR) domain sequence

Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA

Characterization of Rehmannia mosaic virus isolated from chili pepper (Capsicum annuum) in Japan

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