First report of Ageratum yellow vein virus infecting papaya in Lampung, Indonesia

Abstract

Begomovirus attacks on papaya plants have been identi ed in several regions in Indonesia. Detection based on viral nucleic acid in each category of papaya plants in Tanggamus, Pringsewu, West Lampung, Pesawaran, Central Lampung, and Bandar Lampung using universal primers SPG1/SPG2 showed positive results for Begomovirus infection with visible DNA bands measuring ± 900 bp. Direct nucleotide sequencing using SPG1/SPG2 primers for the AC2 dan AC1 genes of the Begomovirus and con rmed by the BLAST program showed that papaya samples were infected with Ageratum yellow vein virus (AYVV). The phylogenetic results show that AYVV from papaya samples has a close relationship with the AYVV group from several other countries with 98% homology. Overall, our study further indicated Ageratum acts as an alternative host and reservoir for Begomovirus.