First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation

Thidiazuron (TDZ) is an active substituted phenyl urea compound that has found a significant role as a plant growth regulator. The most exciting aspect of its function is that it can mimic auxins and cytokinin but is chemically different from these two. Many theories have been put forward, and experiments performed to understand the mode of action of TDZ in callogenesis. One suggested mechanism presents that it works by inhibiting the cytokinin degrading enzymes that compete with cytokinin for an active site on the enzyme. An example is the TDZ‐induced suppressed expression of gibberellic acid (GA) biosynthesis genes encoding GA3 and GA20 oxidases. This is entailed with a slightly increased expression of GA catabolism genes encoding GA20 oxidase. Similarly, one of the recommendations is that TDZ induces the expression of specific genes and transcription regulatory sequences that are either responsible directly for callus formation or in turn induce other auxins or cytokinin for callogenesis. There is no concise review available that discusses the details of TDZ‐induced callus, specifically and other in vitro cultures in general. This review is an attempt to explore all these pathways and mechanisms involved in callogenesis in plants stimulated by TDZ.

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“…For example, the transformation efficiency of Selaginella bryopteris was enhanced TDZ (2 mg/L) to generate hairy roots. 67…”

Section: The Role Of Thidiazuron In Exploiting Hairy Root Culturesmentioning

confidence: 99%

“…Hairy root cultures can produce certain medicinally and commercially when treated with TDZ. For example, the transformation efficiency of Selaginella bryopteris was enhanced TDZ (2 mg/L) to generate hairy roots 67 …”

Section: Applications Of Thidiazuron In Plant Tissue Culturementioning

confidence: 99%

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

Ali

Khan

et al. 2022

Biotech and App Biochem

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“…Chung and coauthors (2016) used jasmonic acid (JA) and salicylic acid (SA) elicitors to enhance biomass accumulation and phenolic compound production in hairy root cultures of Momordica charantia L.. Their results revealed that cultures supplemented with 100 μM JA and SA can significantly enhance the production of phenolic compounds in compared to control cultures [19]. In recent years, there is a growing interest to biotechnological methods such as liquid and hairy root cultures through A. rhizogenes for enhancement of secondary metabolites production in medicinal plants [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Terpenoids Production of Elicited Hairy Root Cultures of Scutellaria bornmuelleri

Gharari

Bagheri

Sharafi

2023

Braz. arch. biol. technol.

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Scutellaria bornmuelleri is recognized as a medicinal plant and a rich source of terpenoids. Hairy Root Cultures (HRCs) along with elicitation of in vitro cultures have been widely considered in order to produce important secondary metabolites such as terpenoids. In the present study, S. bornmuelleri HRCs were established by transformation of stem explants with Agrobacterium rhizogenes strain A4. The effect of different concentrations of sucrose on transgenic root induction and biomass production were investigated. The production and accumulation of terpenoids in HRCs elicited with chitosan (CS), methyl jasmonate (MeJA) and β-cyclodextrin (β-CD) was evaluated using Gas chromatography/mass spectrometry (GC/MS). Among various concentrations of sucrose, half-strength Murashige and Skoog (MS) medium supplemented with 2.5% sucrose resulted in significant increase in transformation frequency and maximum root biomass production. The highest sesquiterpene production was observed in HRCs elicited with 100 mg L -1 CS. However, the highest monoterpene accumulation was occurred in HRCs treated with 100 µM MeJA. Among the combined treatments, the highest sesquiterpene and monoterpene yield was achieved by 0.7 and 7 mM β-CD plus MeJA as well as 50 and 100 mg L -1 CS plus MeJA, respectively. To the best of our knowledge, the results of this study for the first time suggested that eliciting HRCs can be an effective method to accumulate sesquiterpenes in large scale cultures of S. bornmuelleri HRs in bioreactor.

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Micropropagation of Pteridophytes

Suneetha¹,

Hegde²

2022

Ferns

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First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation

Cited by 6 publications

References 27 publications

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

Enhanced Terpenoids Production of Elicited Hairy Root Cultures of Scutellaria bornmuelleri

Micropropagation of Pteridophytes

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