First report of alfalfa mosaic virus on Petroselinum crispum in Turkey

Morca, Ali Ferhan; Coşkan, Sevgi; Akbaş, Birol; Karahan, Aynur Gül

doi:10.1007/s42161-022-01082-6

Cited by 5 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phylogenetic interrelationship created from 21 CP sequences of AMV using the Neighbor-Joining algorithm indicated that both AMV sequences are closely related to alfalfa isolates from Van province (MT210179 and MT210178) and Iğdır province of Turkey (MW882262 and MW882261) ( Earlier literature suggested that AMV has existed for forty years in Turkey and various crops are highly susceptible to this infection (Erdiller and Lesemann, 1985;Çalı, 1990;Yardımcı and Açıkgöz, 1997;Çıtır, 1982;Usta and Güller, 2020). The presence of AMV has been reported in agroeconomic crops and weed such as potato, eggplant, Datura stramonium, bean, alfalfa, husk tomato, and pepper from agricultural region nationwide (Özdemir and Erilmez, 2007;Sertkaya et al, 2017;Ozdemir et al, 2011;Çulal Kılıç and Yardımcı, 2015;Çetinkıran and Baloğlu, 2011;Topkaya, 2020). On the contrary, eggplant plantation regions were screened against AMV infection using RT-PCR technique in Antalya province in 2018-2019, but no AMV infection was detected in eggplant (Fidan and Sarıkaya, 2020).…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic Relationship of Alfalfa Mosaic Virus (AMV) Isolate Identified in Bingöl Province of Turkey

Güller

Usta

KORKMAZ

2022

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

Alfalfa mosaic virus (AMV) is one of the most important viral diseases of alfalfa plant among the forage crop, causing significant annual economic losses. The agent is also of potential importance to other cultivars such as tomatoes, potatoes, and peppers in most cases. The identification and phylogenetic relationships of AMV were carried out by reverse-transcription polymerase chain reaction (RT-PCR), following by bacterial cloning. The cDNA of alfalfa samples (12) were subjected to RT-PCR tests using primer pairs, specific for the capsid protein gene (CP) of AMV, resulting in a DNA fragment of approximately 700 bp as expected. The amplicons were directly cloned and then resulting sequences were deposited in GenBank (Acc. No: MW962977, MW962976). The BLASTn analysis of both sequences demonstrated that AMV virus isolates from alfalfa were highly similar to other AMV isolates from various crops in the world, with nucleotide identity ranging from 97 to 99.37%. The results of phylogenetic dendrogram based on CP gene sequences clearly suggested that our isolates are closely related to four AMV isolates from alfalfa in Turkey. To our knowledge, this study is the first report of molecular phylogeny and AMV presence in alfalfa exhibiting yellowing, mottling, and leaves abnormalities in Bingöl province, Turkey.

show abstract

Section: Resultsmentioning

confidence: 99%

Phylogenetic Relationship of Alfalfa Mosaic Virus (AMV) Isolate Identified in Bingöl Province of Turkey

Güller

Usta

KORKMAZ

2022

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

show abstract

“…PVY infection was detected in potato samples at a rate of 49.5%, followed by PLRV and AMV infections at 5.4% (Carpar and Sertkaya, 2016). Furthermore, the presence of AMV was serologically detected in pepper plants in Gaziantep, Şanlıurfa, and Kahramanmaraş 9 (Buzkan et al, 2006), Amaranthus retroflexus, pepper, Malva sylvestris, Solanum nigrum, and S. oleraceus in Hatay (Sertkaya and Özdağ, 2017;, tobacco in Samsun (Erdem, 2010), and pepino in Uşak (Özdemir and Erilmez, 2012). AMV-infected pepper plants were reported in Samsun by Arli-Sökmen et al (2005), in Adana by Çetinkıran and Baloğlu (2011), and in Kahramanmaraş by Demir (2005).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Coat protein of alfalfa mosaic alfamovirus (AMV) from Türkiye: genetic inference and in silico docking analysis for potential antiphytoviral purposes

DEMİREL,

GÜLLER,

USTA

et al. 2024

Not Bot Horti Agrobo

View full text Add to dashboard Cite

In 2021, a study was conducted in the Denizli region of Türkiye to investigate the phylogenetic relationship and presence of alfalfa mosaic alfamovirus (AMV) infecting pepper plants exhibiting viral disease symptoms. A total of 57 samples were collected, of which twenty-four tested positive for AMV with reverse transcriptase polymerase chain reaction (RT-PCR) assays. Samples from pepper plants displaying virus symptoms gave positive bands on the agarose gel, while healthy plants yielded negative results. One of the positive samples was randomly selected, cloned and sequenced. This sequence of the Denizli AMV isolate (753 bp) was recorded in the GenBank database with accession number OQ845956. The nucleotide sequence showed a high nucleotide consensus of 97%-99% compared with the nucleotide sequences of the same variants from different origins in GenBank. According to the phylogenetic tree generated through the Neighbour Joining (NJ) method, this AMV isolate belongs to the same group as Iranian isolates from various of hosts. Furthermore, in silico docking analysis of the coat protein (CP) of the AMV isolate and promising 12 essential oil compounds was performed to enable potential antiviral drug development. Docking study showed that eucalyptol, eugenol and carvacrol can make important contributions to the advancement of drug-based strategies for the managing of plant viruses by interacting with the virus coat protein of high binding energies, -5.3, -5.2 and -5.0 kcal mol-1, respectively. Although the presence of AMV in Denizli province has been reported previously, this study reports the phylogenetic relationships and docking analysis of the new AMV isolate in pepper crops.

show abstract

“…Recently, the research in the world shown that CMV subgroup I divided into two subgroups (Ia and Ib) with the analysis of non-protein coding regions at the 5' end and the CP gene [6]. Previous studies have shown the presence of CMV infections in various hosts in Turkey, as described by several researchers [29,32,36,37,38]. A few studies have shown that subgroup IA isolates are frequently observed in molecular investigations carried with CMV [34,39,40].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province

Güller,

Demirel,

Usta

et al. 2024

Türk Doğa Ve Fen Dergisi

View full text Add to dashboard Cite

The cucumber plant (Cucumis sativus L) is an important cultivated plant produced worldwide. Cucumber mosaic virus (Cucumber mosaic virus, CMV), one of the common viral diseases, causes economic losses by reducing the yield and quality of the cucumber plant. In the observations performed in Diyarbakır in September 2021, cucumber plants showing virus-like symptoms such as mosaic, irregular yellowish spots, and deformity on the leaves were observed. Symptomatic and non-symptomatic samples were collected and subjected to a polymerase chain reaction (PCR) using CMV-specific primers, and the produced DNA bands were visualized on an agarose gel. CMV infection was detected in seven of the 15 samples. Bacterial cloning and sequencing of a randomly selected specimen determined that the CMV partial coat protein gene was 593 bp long and was registered in the NCBI database with the accession number MW962979.1. According to the phylogenetic tree performed with different isolates of CMV, Diyarbakir CMV isolate clustered with CMV isolates from Australia, Israel, Spain, Hungary, Japan, and Korea forming Subgroup IA. The presence of CMV and group/subgroup diagnosis in cucumber plants grown in the Diyarbakır region were confirmed molecularly for the first time by this study.

show abstract

First report of alfalfa mosaic virus on Petroselinum crispum in Turkey

Cited by 5 publications

References 3 publications

Phylogenetic Relationship of Alfalfa Mosaic Virus (AMV) Isolate Identified in Bingöl Province of Turkey

Phylogenetic Relationship of Alfalfa Mosaic Virus (AMV) Isolate Identified in Bingöl Province of Turkey

Coat protein of alfalfa mosaic alfamovirus (AMV) from Türkiye: genetic inference and in silico docking analysis for potential antiphytoviral purposes

Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province

Contact Info

Product

Resources

About