First report of an alphasatellite associated with Okra enation leaf curl virus

Chandran, Sam Aldrin; Packialakshmi, R. M.; Subhalakshmi, K.; Prakash, Celine; Poovannan, K.; Prabu, A. Nixon; Pandi, Gopal; Usha, R.

doi:10.1007/s11262-013-0898-y

Cited by 22 publications

(9 citation statements)

References 12 publications

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“…However, recently the first report of an alphasatellite associated with OELCuV, an Indian isolate is similar to Hollyhock yellow vein virus‐associated symptomless alphasatellite (HoYVSA; Chandran et al. ). Phylogenetic analysis of these two alphasatellites and other related alphasatellites shows that both alphasatellite presumably associated with OELCuD are entirely different and distantly related.…”

Section: Discussionmentioning

confidence: 99%

Recombination Among Begomoviruses on Malvaceous Plants Leads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan

Serfraz

Amin

Akhtar

et al. 2015

Journal of Phytopathology

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Whitefly transmitted begomoviruses (family Geminiviridae) are the major reason for significant yield losses of dicotyledonous crops in tropics and subtropics. Okra (Abelmoschus esculentus) is one of the important vegetable crops, and leaf curl disease caused by geminiviruses is the most important limiting factor for its production in Pakistan. Here, we report a new species of okra-infecting begomovirus in south-eastern region of Pakistan and the name Okra enation leaf curl virus (OELCuV) complex is proposed. This okra enation leaf curl disease complex (OELCuD) in Pakistan is found to be associated with Ageratum conyzoides symptomless alphasatellite (ACon-SLA). All efforts to clone the betasatellite were unsuccessful. Comprehensive sequence analyses suggest that intermalvaceous recombination between okra and cotton-infecting begomoviruses resulted in the evolution of the new species. Surprisingly, Bhendi yellow vein mosaic virus (BY-VMV) which has not been reported previously from Pakistan is the major parent while Cotton leaf curl Multan virus (CLCuMV) acts as a distant parent of the virus. Comparative recombination analysis also reveals that okrainfecting begomoviruses from south and north-western India is causing OELCuD in the Pakistan by recombining with CLCuMV at the Rep (1964Rep ( -1513. Recombination is common among geminiviruses and recombining of BYVMV and CLCuMV resulted in a new species: OELCuV. To the best of our knowledge, this evolution of a new species of okra-infecting begomovirus is the first report of intermalvaceous recombination where Rep acts as the target region. nent (betasatellite, alphasatellites; Mansoor et al.

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Section: Discussionmentioning

confidence: 99%

Recombination Among Begomoviruses on Malvaceous Plants Leads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan

Serfraz

Amin

Akhtar

et al. 2015

Journal of Phytopathology

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“…However, RNAi silencing suggested its probable role in overcoming the host-defense (Nawaz-ul-Rehman et al, 2010). An alphasatellite DNA of 1,350 nucleotides was found associated with OELCuV in okra and showed 79.7% similarity to Hollyhock yellow vein virus-associated symptomless alphasatellite (HoYVSLA) (Chandran et al, 2013). …”

Section: Begomoviruses Infecting Okra and Need Of Biotechnological Inmentioning

confidence: 99%

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

et al. 2017

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Despite the importance of okra, as one of the important vegetable crop, very little attention has been paid to its genetic improvement using advanced biotechnological tools. The exploitation of marker assisted breeding in okra is often limited due to the availability of a few molecular markers, the absence of molecular genetic-map(s), and other molecular tools. Chromosome linkage-groups were not yet constructed for this crop and reports on marker development are very scanty and mostly hovering around cultivar characterization. Besides, very little progress has been observed for transgenic development. However, high throughput biotechnological tools like chromosome engineering, RNA interference (RNAi), marker-assisted recurrent selection (MARS), genome-wide selection (GWS), targeted gene replacement, next generation sequencing (NGS), and nanobiotechnology can provide a rapid way for okra improvement. Further, the etiology of many deadly viral diseases like the yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCV) in okra is broadly indistinct and has been shown to be caused by various begomovirus species. These diseases cause systemic infections and have a very effective mode of transmission; thus, preventing their spread has been very complicated. Biotechnological interventions have the potential to enhance okra production even under different viral-stress conditions. In this background, this review deals with the biotechnological advancements in okra per se along with the begomoviruses infecting okra, and special emphasis has been laid on the exploitation of advanced genomic tools for the development of resistant varieties.

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“…Moreover, some of the fragments showed significantly high identities to the nanovirus-like particle rep gene sequences (for replication associated protein), one of which has been previously shown to be associated with okra (Briddon et al 2004). Unexpectedly, none of these cloned fragments showed significant identity to the only alphasatellite reported to be associated with OELCuV and with the betasatellite associated with BYVMV (Chandran et al 2013;Jose and Usha 2003). It can thus be concluded that diverse begomoviruses and satellites are associated with BYVMD in India.…”

Section: Introductionmentioning

confidence: 89%

“…More recently, two other reports; the bipartite begomovirus, Bhendi yellow vein Delhi virus (BYVDV, Venkataravanappa et al 2012) and Bhendi yellow vein Bhubaneswar virus (BYVBV, Venkataravanappa et al 2013), both of which are associated with symptoms resembling BYVMD in okra, have appeared in the literature. There is also a recent report of an alphasatellite associated with Okra enation leaf curl virus (OELCuV) from western India (Chandran et al 2013). However, there is no published report on OELCuV.…”

Section: Introductionmentioning

confidence: 99%

Diverse and recombinant begomoviruses and various satellites are associated with Bhendi yellow vein mosaic disease of okra in India

Rishishwar

Mazumdar

Dasgupta

2015

J. Plant Biochem. Biotechnol.

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Bhendi/Okra, a common vegetable in the Indian subcontinent, is highly susceptible to the viral disease Bhendi yellow vein mosaic disease (BYVMD), resulting in considerable yield loss to the crop. As a first step towards formulating novel strategies against BYVMD, the identities of the viruses and associated satellite molecules were investigated in BYVMD-affected okra leaf samples taken from ten widely separated locations in India. The amplified nucleotide sequence representing the viral coat protein from the samples indicated the presence of the begomoviruses Bhendi yellow vein mosaic virus (BYVMV) in seven and Mesta yellow vein mosaic virus (MeYVMV) in three samples. Subsequent analysis of the full-length viral sequences revealed the presence of a recombinant viral DNA, having MeYVMVand Malvastrum yellow vein Yunnan virus (MYVYNV), a virus not previously reported from India, as parents. Betasatellite sequences were very similar to previous reports. Analysis of the symptomatic samples by Rolling circle amplification showed, in addition to BYVMV-like sequences, begomoviral sequences reported earlier from cotton and mint and alphasatellites reported previously from cotton and Cyamopsis tetragonoloba were also present. This study indicated that the diversity of begomoviruses and satellites associated with BYVMD is much higher than previously thought.

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First report of an alphasatellite associated with Okra enation leaf curl virus

Cited by 22 publications

References 12 publications

Recombination Among Begomoviruses on Malvaceous Plants Leads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan

Recombination Among Begomoviruses on Malvaceous Plants Leads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

Diverse and recombinant begomoviruses and various satellites are associated with Bhendi yellow vein mosaic disease of okra in India

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