2009
DOI: 10.1002/bmc.1312
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First report of an antifungal amidase from Peltophorum ptercoarpum

Abstract: A 60 kDa antifungal amidase was purified from Peltophorum pterocarpum [corrected] seeds using an isolation procedure that entailed ion-exchange chromatography on Q-Sepharose, ion-exchange chromatography on DEAE-cellulose and FPLC-gel filtration on Superdex 75. Unlike most other antifungal proteins isolated previously, it was adsorbed on Q-Sepharose and DEAE-cellulose. The isolated protein, designated as peltopterin, exhibited an N-terminal amino acid sequence closely resembling those of amidases. It exhibited … Show more

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Cited by 13 publications
(6 citation statements)
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“…These experimental results get nice correlation with a previous investigation 30 where antimicrobial activity of methanol extract of P. pterocarpum flowers was found against B. subtilis, S. aureus, S. epidermidis, E. faecalis, E. coli, P. aeruginosa, K. pneumoniae, P. vulgaris, and C. albicans at the concentration of 5 mg/mL. Lam & Ng 26 isolated an amidase, peltopterin, from P. pterocarpum seeds and found its antifungal activity against Rhizotonia solani in the pH range 0-14 and temperature range of 25-100°C 26 . This amidase impeded mycelial growth of this fungus with an IC 50 of 0.65 μM.…”
Section: Chemical Constituents: Chemical Constituents (Structures Shosupporting
confidence: 89%
“…These experimental results get nice correlation with a previous investigation 30 where antimicrobial activity of methanol extract of P. pterocarpum flowers was found against B. subtilis, S. aureus, S. epidermidis, E. faecalis, E. coli, P. aeruginosa, K. pneumoniae, P. vulgaris, and C. albicans at the concentration of 5 mg/mL. Lam & Ng 26 isolated an amidase, peltopterin, from P. pterocarpum seeds and found its antifungal activity against Rhizotonia solani in the pH range 0-14 and temperature range of 25-100°C 26 . This amidase impeded mycelial growth of this fungus with an IC 50 of 0.65 μM.…”
Section: Chemical Constituents: Chemical Constituents (Structures Shosupporting
confidence: 89%
“…The antifungal potency toward F. oxysporum was reflected in an IC 50 value of 8.3 μ m . The antifungal potency of CsPR10 is similar to that displayed by other antifungal proteins (Lam & Ng 2009, 2010; Fang et al. 2010; Ye & Ng 2009).…”
Section: Resultssupporting
confidence: 56%
“…Its antifungal activity was thermostable and pH stable. It causes chitin deposition at hyphal tips in R. solani (Lam and Ng 2010b). Ib-AMPs from Impatiens balsamina seeds are 20-mer antifungal and antibacterial peptides with a well-defined loop structure stabilized by two disulfide bonds (Patel et al 1998).…”
Section: Other Antifungal Proteinsmentioning
confidence: 99%