First Report of Apple Latent Spherical Virus Infecting <i>Angelica sinensis</i> in China

Jin, Weijie; Zhang, Yubao; Su, Xuesi; Tang, Guoliang; Xie, Zhongkui; Wang, Ruoyu; Wang, Yajun; Zhao, Xia

doi:10.1094/pdis-08-22-1756-pdn

Cited by 3 publications

(1 citation statement)

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“…Angelica acutiloba Kitagawa is of the same botanical family ( Umbelliferae ) and genus as Angelica sinensis Diels (ASD) [ 26 , 27 ], and is often used as an alternative to ASD in Chinese herbal medicine [ 28 ]. Currently, the dried roots of A. acutiloba [ 29 ] are commonly used to treat irregular menstruation, dysmenorrhea, blood deficiency amenorrhea, abdominal pain, and constipation [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Angelica acutiloba Kitagawa flower induces A549 cell pyroptosis via the NF-κB/NLRP3 pathway for anti-lung cancer effects

Chen,

Zhu,

Che

et al. 2023

Cell Div

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Angelica acutiloba Kitagawa, a traditional medicinal herb of the Umbelliferae family, has been demonstrated to have anticancer activity. In this study, we investigated the anti-lung cancer effects of two compounds extracted from A. acutiloba flowers: kaempferol-3-O-α-L-(4″-E-p-coumaroyl)-rhamnoside (KAE) and platanoside (PLA). MTT, cell colony formation, and cell migration (scratch) assays revealed that both KAE (100 μM) and PLA (50 μM and 100 μM) inhibited the viability, proliferation, and migration of A549 cells. Dichlorodihydrofluorescein diacetate assays showed that KAE and PLA also induced the generation of reactive oxygen species in A549 cells. Morphologically, A549 cells swelled and grew larger under treatment with KAE and PLA, with the most significant changes at 100 μM PLA. Fluorescence staining and measurement of lactate dehydrogenase release showed that the cells underwent pyroptosis with concomitant upregulation of interleukin (IL)-1β and IL-18. Furthermore, both KAE and PLA induced upregulation of NF-κB, PARP, NLRP3, ASC, cleaved-caspase-1, and GSDMD expression in A549 cells. Subsequent investigations unveiled that these compounds interact with NLRP3, augment NLRP3’s binding affinity with ASC, and stimulate the assembly of the inflammasome, thereby inducing pyroptosis. In conclusion, KAE and PLA, two active components of A. acutiloba flower extract, had significant anti-lung cancer activities exerted through regulation of proteins related to the NLRP3 inflammasome pathway.

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