First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt

Seleim, Mohamed A. A.; Abo-Elyousr, Kamal A. M.; Abd-El-Moneem, Kenawy M.; Saead, Farag A.

doi:10.5423/ppj.nt.10.2013.0101

Cited by 17 publications

(10 citation statements)

References 17 publications

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“…A wilting score was recorded once a week for 4 weeks on a scale of 0 to 5, where 0 = no visible wilt, 1 = one leaf wilted, 2 = two or three leaves wilted, 3 = all except top leaves wilted, 4 = all leaves wilted, and 5 = dead (Wang et al 2000). Percentage of wilted plant (W) was calculated as described by Winstead and Kelman (1952) and Seleim et al (2014) using the formula W = [(Nt -Nh)/Nt] 9 100, where Nt is number of total plants and Nh is number of unwilted plants. W was arcsine square root-transformed for data normalization before QTL analysis using the PROC MIXED procedure of SAS (SAS 9.2, SAS Institute, Cary, USA).…”

Section: Caps Marker Designmentioning

confidence: 99%

Construction of a single nucleotide polymorphism marker based QTL map and validation of resistance loci to bacterial wilt caused by Ralstonia solanacearum species complex in tomato

et al. 2020

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Bacterial wilt (BW), caused by Ralstonia solanacearum species complex is one of the major biotic factors limiting tomato production in the humid tropics. Pyramiding of resistance genes through marker-assisted selection is an efficient way to develop durable BW resistant cultivars. Tomato line 'Hawaii 7996' (H7996) is a stable and robust resistance source against various strains of the species complex. Major BW resistance quantitative trait loci (QTLs) Bwr-12 and Bwr-6, and several minor or strain specific QTLs have been coarse-mapped in this line, but none has been fine-mapped and validated. The objective of the current study was to construct a high density genetic map using single-nucleotide polymorphism (SNP) markers derived from genotyping-bysequencing, fine-map Bwr-12 and Bwr-6 and determine the effects of these QTLs using a near isogenic line (NIL) population. A high density genetic map using 1604 SNP markers with an average distance of 0.82 cM was developed for 188 F 9 recombinant inbred lines derived from the cross H7996 9 WVa700. A total of seven QTLs associated with BW resistance to race 1-phylotype I (R. pseudosolanacearum) or/and race 3-phylotype II (R. solanacearum) strains were located on chromosomes 6 (Bwr-6.1, 6.2, 6.3 and 6.4) and 12 (Bwr-12.1, Bwr-12.2 and Bwr-12.3) with logarithm of odds (LOD) scores of 6.2-15.6 and 6.2-31.1, explaining 14.2-33.4% and 15.9-53.9% of the total phenotypic variation contributed from H7996, respectively. To validate the genetic effects of the two QTL regions, a set of 80 BC 3 F 3 NILs containing different sections of Bwr-6 with or without Bwr-12 was phenotyped for disease severity after challenge with either race 1-phylotype I Pss4 or race 3-phylotype II Pss1632 BW strains over two seasons. Bwr-6.1 specific to Pss4 and Bwr-6.3 specific to Pss1632 were mapped to an interval of 5.0 cM (P \ 0.05) between 6_33,444,000_SLM6-47 and 6_33,868,000_SLM6-124 SNP marker, and to 2.7 cM (P \ 0.01) between positions 6_35,949,000 _SLM6-107 to 6_36,750,000_SLM6-82 marker, respectively. In addition, the specific effect of Bwr-12 for resistance to Pss4 (LOD score of 5.8-16.1, P \ 0.01) was confirmed.

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Section: Caps Marker Designmentioning

confidence: 99%

Construction of a single nucleotide polymorphism marker based QTL map and validation of resistance loci to bacterial wilt caused by Ralstonia solanacearum species complex in tomato

et al. 2020

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“…5 ml of TZC solution filter sterilized was added to the autoclaved medium to give final concentration of 0.005%) according to the procedure of Seleim, et al [12]. The plates were incubated at 28 o C ± 2°C for 24-48 hr.…”

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confidence: 99%

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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Article HistoryRalstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5-1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants.

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“…H 2 S production was noticed by the appearance of black precipitation. Seleim et al (2014) also found positive motility for all R. solanacearum isolates from wilt infected tomato plants. In the biochemical test, R. solanacearum strain BD isolated from wilt disease of papaya showed positivity to methyl red and Kovac oxidase which is confirmatory with the findings of Zhang et al (2011) .…”

Section: Discussionmentioning

confidence: 75%

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

Hossain

Billah

Ali

et al. 2021

Saudi Journal of Biological Sciences

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Ralstonia solanacearum is a harmful pathogen that causes severe wilt disease in several vegetables. In the present study, we identified R. solanacearum from wilt of papaya by 16S rRNA PCR amplification. Virulence ability of R. solanacearum was determined by amplification of approximately 1500 bp clear band of hrpB gene. Further, in-vitro seed germination assay showed that R. solanacearum reduced the germination rate up to 26.21%, 34% and 33.63% of cucumber, bottle guard and pumpkin seeds, respectively whereas shoot and root growth were also significantly decreased. Moreover, growth inhibition of R. solanacearum was recorded using antibacterial compound from medicinal plant and antagonistic B. subtilis. Petroleum ether root extract of Rauvolfia serpentina showed highest 22 ± 0.04 mm diameter of zone of inhibition where methanolic extract of Cymbopogon citratus and ethanolic extract of Lantana camara exhibited 20 ± 0.06 mm and 20 ± 0.01 mm zone of inhibition against R. solanacearum , respectively . In addition, bioactive compounds of B. subtilis inhibited R. solanacearum growth by generating 17 ± 0.09 mm zone of inhibition. To unveil the inhibition mechanism, we adopted chemical-protein interaction network and molecular docking approaches where we found that, rutin from C. citratus interacts with citrate (Si)-synthase and dihydrolipoyl dehydrogenase of R. solanacearum with binding affinity of −9.7 kcal/mol and −9.5 kcal/mol while quercetin from B. subtillis interacts with the essential protein F0F1 ATP synthase subunit alpha of the R. solancearum with binding affinity of −6.9 kcal/mol and inhibit the growth of R. solanacearum . Our study will give shed light on the development of eco-friendly biological control of wilt disease of papaya.

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First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt

Cited by 17 publications

References 17 publications

Construction of a single nucleotide polymorphism marker based QTL map and validation of resistance loci to bacterial wilt caused by Ralstonia solanacearum species complex in tomato

Construction of a single nucleotide polymorphism marker based QTL map and validation of resistance loci to bacterial wilt caused by Ralstonia solanacearum species complex in tomato

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

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