First Report of Bacterial Wilt of Amaranth (<i>Amaranthus cruentus</i>) Caused by <i>Ralstonia solanacearum</i> in Benin

Sikirou, Rachidatou; Zocli, Bruno; Afari‐Sefa, Victor; Honfoga, Judith; Azoma, K.; Chen, J.-R.; Paret, Mathews L.; Bihon, Wubetu

doi:10.1094/pdis-07-18-1140-pdn

Cited by 9 publications

(7 citation statements)

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“…The occurrence of R. pseudosolanacearum in other ornamental plants associated with lethal wilting was previously reported for Osteospermum , Petunia , Calibrachoa , Verbena , Lobelia and Amaranthus spp. (Weibel et al ., 2016; Sikirou et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

et al. 2020

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The Ralstonia solanacearum species complex (RSSC) can cause bacterial wilt in a wide variety of plant species, including a number of ornamental glasshouse crops. Recently in Europe, ornamental rose plants for the production of cut flowers and propagation materials have been strongly affected by Ralstonia pseudosolanacearum, phylotype I, biovar 3. To test for the presence of the pathogen in the glasshouse, sampling of water from a drainage gutter or well may be an efficient strategy since it is known that RSSC can be released from infected root systems in the water. A protocol was developed to detect low densities of R. pseudosolanacearum in drain water collected from rose growers. Drain water was filtered through a bacterial filter, the filtrate was collected and target bacteria enriched for 48 h in Semi‐selective Medium South Africa (SMSA) broth supplemented with sterilized tomato plant extracts. DNA extracted from the enrichment broth was analysed using a TaqMan test in a duplex format, based on specific egl sequences of RSSC and the use of an extraction and amplification control. The optimized protocol had a detection level of ≤1–10 colony forming units of R. pseudosolanacearum in drain water.

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Section: Introductionmentioning

confidence: 99%

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

et al. 2020

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“…3). Bacterial wilt can cause up to 100% crop losses and is consequently considered as one of the most serious diseases for solanaceous crops, including tomato, eggplant, sweet pepper, and African eggplant (Charrier et al 1997;Fufa et al 2009) and was also reported to affect Amaranthus (Sikirou et al 2019) and cucurbit crops (Wicker et al 2002) in tropical regions. The pathogen enters the plant through the roots and colonizes xylem tissue, thereby reducing the movement of water through the plant.…”

Section: Bacterial Wiltmentioning

confidence: 99%

Tapping the potential of grafting to improve the performance of vegetable cropping systems in sub-Saharan Africa. A review

Nordey¹,

Schwarz

Kenyon

et al. 2020

Agron. Sustain. Dev.

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Vegetable grafting is developing worldwide to cope with biotic and abiotic stresses. This technique faces strong regional disparities and its potential to improve crop performances is currently underexploited in sub-Saharan Africa. This review explores the potential of grafting to increase and secure vegetable production in challenging production conditions in sub-Saharan Africa and to identify the obstacles to its adoption. The major conclusions that can be drawn from the review are (1) the capacity of vegetable grafting to overcome several agronomic challenges commonly encountered in sub-Saharan Africa particularly for soilborne diseases; (2) grafting does not systematically lead to higher yields since the performance of grafted plants is closely linked with the combination of scion-rootstock chosen; (3) resistant rootstocks to the local soilborne pathogens could be identified through short-term laboratory and field experiments whereas the improvement of the tolerance to abiotic stresses would require deeper knowledge on underlying mechanisms; (4) the significant increase in the cost of using grafted plants are warranted in systems where there are no other technical alternatives to manage biotic and abiotic stresses, where profitability per plant is high, or where the use of grafted plants makes it possible to reach a profitable niche market such as off-season or organic products; and (5) vegetable grafting should therefore be promoted by providing evidence for its profitability in different production systems (open field and greenhouse cultivation) combined with supply-side interventions to build or strengthen the capacity of nursery operators to provide a continuous supply of good-quality grafted plants.

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“…Unfortunately, most such vegetables are damaged by numerous plant pathogens among which bacteria are of importance given the severity of their adverse effect upon infection. Recently, bacterial wilt caused by Ralstonia solanacearum was reported as a limiting factor in Solanaceae production in Benin [13], and bacterial wilt incidence of 70% on tomato [14] and 75.2% on Gboma [15] were reported. Globally, economic losses due to R. solanacearum in potato cultivation were estimated at 950 million USD [16].…”

Section: Introductionmentioning

confidence: 99%

“…Globally, economic losses due to R. solanacearum in potato cultivation were estimated at 950 million USD [16]. In addition to the many host crops from the Solanaceae, R. solanacearum was reported for the first time in Benin on Amaranthaceae crops [13], with 72.4% incidence on amaranth and an equivalent level of yield loss due to entire plant damage.…”

Section: Introductionmentioning

confidence: 99%

Screening of Amaranthus sp. Varieties for Resistance to Bacterial Wilt Caused by Ralstonia solanacearum

et al. 2021

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Bacterial wilt, caused by Ralstonia solanacearum, is an emerging constraint in amaranth production in Benin. Host resistance is the most sustainable disease control measure. Ten amaranth varieties including A2002, Bresil (B) -Sel, Madiira 2, AC-NL, GARE ES13-7, Madiira 1, UG-AMES13-2, AM-NKGN, IP-5-Sel and a local variety from Benin were screened for resistance to bacterial wilt. The study was conducted in a screen house and in the naturally contaminated open field during a consecutive rainy and dry season using a randomized complete block design with four and three replications, respectively. In the screen house, plants were inoculated by drenching a 40 mL of bacterial suspension containing 108 CFU/mL of R. solanacearum strain NCBI 5 GenBank N° MH397250 at the collar region. The bacterial wilt incidence (BWI) and the area under the disease progress curve (AUDPC) suggested differential reactions of amaranth varieties to the pathogen. BWI and AUDPC were low for UG-AMES13-2, moderate for Madiira 2, AM-NKGN and the local variety and very high for A2002, Bresil (B) -Sel, AC-NL, GARE ES13-7, Madiira 1 and IP-5-Sel. The World Vegetable Center’s UG-AMES13-2 can be considered as first choice, which is resistant to R. solanacearum, and should be scaled up for seed production towards supporting farmers.

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First Report of Bacterial Wilt of Amaranth (Amaranthus cruentus) Caused by Ralstonia solanacearum in Benin

Cited by 9 publications

References 1 publication

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

Tapping the potential of grafting to improve the performance of vegetable cropping systems in sub-Saharan Africa. A review

Screening of Amaranthus sp. Varieties for Resistance to Bacterial Wilt Caused by Ralstonia solanacearum

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