First Report of Bean Common Mosaic Necrosis Virus Infecting Soybean in Korea

Jang, Yunwoo; Jo, Yeonhwa; Cho, W K; Choi, Hoseong; Yoon, Young Mi; Lim, Seungmo; Lee, Y H; Bae, Ju Young; Lee, B C

doi:10.1094/pdis-09-17-1474-pdn

Cited by 11 publications

(3 citation statements)

References 2 publications

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“…In addition, we revealed that PeMoV and PSV were also frequently identified in the Republic of Korea. Moreover, we identified CMV, TSWV, WVMV, BCMV, and BCMNV infecting soybean in Korea, as previously reported [ 35 , 36 ]. Of them, we report infection of WVMV in the soybean for the first time in Korea and the world.…”

Section: Discussionsupporting

confidence: 75%

Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing

Yoon

Jang

et al. 2020

Microorganisms

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Soybean (Glycine max L.) is one of the most important crop plants in the Republic of Korea. Here, we conducted a soybean virome study. We harvested a total of 172 soybean leaf samples showing disease symptoms from major soybean-growing regions in the Republic of Korea. Individual samples were examined for virus infection by RT-PCR. Moreover, we generated eight libraries representing eight provinces by pooling samples and four libraries from single samples. RNA-seq followed by bioinformatics analyses revealed 10 different RNA viruses infecting soybean. The proportion of viral reads in each transcriptome ranged from 0.2 to 31.7%. Coinfection of different viruses in soybean plants was very common. There was a single dominant virus in each province, and this geographical difference might be related to the soybean seeds that transmit viruses. In this study, 32 viral genome sequences were assembled and successfully used to analyze the phylogenetic relationships and quasispecies nature of the identified RNA viruses. Moreover, RT-PCR with newly developed primers confirmed infection of the identified viruses in each library. Taken together, our soybean virome study provides a comprehensive overview of viruses infecting soybean in eight geographical regions in the Republic of Korea and four single soybean plants in detail.

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Section: Discussionsupporting

confidence: 75%

Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing

Yoon

Jang

et al. 2020

Microorganisms

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“…Of these, the soybean mosaic virus (SMV), the soybean yellow mottle mosaic virus (SYMMV), and the soybean yellow common mosaic virus (SYCMV) have been identified as major viruses infecting soybean in Korea [7]. Other viruses infecting soybean in Korea include the peanut mottle virus (PeMV), the soybean dwarf virus, the clover yellow vein virus, the bean common mosaic virus, the peanut stunt virus, the tomato spotted wilt virus, and the bean common mosaic necrosis virus [8][9][10][11][12][13][14]. Although various RNA viruses infecting soybean have been identified, none of the DNA viruses infecting soybean have been reported in Korea.…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence of a Novel Monopartite Mastrevirus, Soybean Geminivirus B, Isolated from Soybean (Glycine max (L.) Merrill)

Choi

Hong

et al. 2022

Plants

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Soybean is one of the most important crops in Korea. To identify the viruses infecting soybean, we conducted RNA sequencing with samples displaying symptoms of viral disease. A contig displaying sequence similarity to the known Geminivirus was identified. A polymerase chain reaction (PCR) using two different pairs of back-to-back primers and rolling circle amplification (RCA) confirmed the complete genome of a novel virus named soybean geminivirus B (SGVB), consisting of a circular monopartite DNA genome measuring 2616 nucleotides (nt) in length. SGVB contains four open reading frames (ORFs) and three intergenic regions (IRs). IR1 includes a nonanucleotide origin of replication in the stem-loop structure. Phylogenetic and BLAST analyses demonstrated that SGVB could be a novel virus belonging to the genus Mastrevirus in the family Geminiviridae. We generated infectious clones for SGVB by adding a copy of the IR1 region of SGVB, comparing the V-ori in addition to the full-length genome of SGVB. Using the infectious clones, we observed chlorosis and leaf curling with a latent infection in the inoculated Nicotiana benthamiana plants, while none of the inoculated soybean plants showed any visible symptoms of disease. This study provides the complete genome sequence and infectious clones of a novel Mastrevirus referred to as SGVB from soybean in Korea.

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“…Yield loss can reach 100% in severely BCMVinfected bean fields (Saqib et al 2010;Li et al 2014), and the virus is internationally distributed. Isolates of BCMV have been identified and characterized from different countries, including USA, India, China, Iran, South Korea, Turkey, and Australia, based on analysis of complete or partial genome sequences (Flores-Estévez et al 2000;Saqib et al 2005;Damayanti et al 2008;Cui et al 2014;Seo et al 2015;Jang et al 2018). BCMV is divided into strains based on host symptoms, serological relationships, high performance liquid chromatography peptide mapping, and genome sequence (Drijfhout et al 1978;McKern et al 1992;Mink and Silbernagel 1992;Feng et al 2014aFeng et al , 2014bLi et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of a recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of bean common mosaic virus

et al. 2021

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Bean common mosaic virus (BCMV) is one of the most widespread and damaging viruses of cultivated legumes in the world. In addition to serious yield reduction and germplasm decline, BCMV infection also makes legumes more vulnerable to other pathogens. Early diagnosis of the virus is particularly important in limiting its spread. Recombinase polymerase amplification (RPA) is a novel isothermic amplification technology. The whole reaction can be done outside the laboratory environment after the nucleic acid sample is obtained. In this study, we established a rapid and sensitive RPA combined with the lateral flow dipstick (LFD) assay for detection of BCMV, based on the conserved BCMV coat protein (CP) gene sequence. Specific primers and a probe were designed, which amplify ~ 150 bp CP fragments from BCMV-infected samples under a constant temperature of 37 °C for 20 min. The end-labeled amplification products were detected by high-affinity LFD within 5 min. Sensitivity of this RPA-LFD assay was 1000 times greater than that of the conventional polymerase chain reaction (PCR) assay. Furthermore, when the primers/probe were used against related potyviruses including soybean mosaic virus (SMV), bean yellow mosaic virus (BYMV), and turnip mosaic virus (TuMV), the three potyviruses were not detected, indicating that the assay was BCMV species-specific. The RPA-LFD assay was also successfully applied for the detection of seed-borne BCMV in beans. The RPA-LFD assay has great potential application in the rapid diagnosis of BCMV in the field.

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First Report of Bean Common Mosaic Necrosis Virus Infecting Soybean in Korea

Cited by 11 publications

References 2 publications

Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing

Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing

Complete Genome Sequence of a Novel Monopartite Mastrevirus, Soybean Geminivirus B, Isolated from Soybean (Glycine max (L.) Merrill)

Development of a recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of bean common mosaic virus

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