FIRST REPORT OF Begomovirus INFECTION ON PAPAYA IN BENGKULU, INDONESIA

Abstract

First report of Begomovirus infection on papaya in Bengkulu, Indonesia. A field survey was conducted during 2019, wefound a severe systemic yellow mosaic, striped green mosaic on leaves petiole, green spots on the fruit of papaya, leafmalformation, and stunting symptoms on three papaya cultivation area in Rejang Lebong, Kepahiang, Bengkulu Tengah, andSeluma, Bengkulu Province, Indonesia. A begomo-like virus was inferred to be the possible cause of the virus-disease-likesymptoms. The study aimed to identify the causal of those typical symptoms on papaya. PCR using universal primer fortranscriptional activator protein (TrAp) and replication-associated protein (Rep) gene of Begomovirus successfully amplifiedthe DNA fragments of 900 bp in all 10 detected samples, except for samples with leaf malformation and stunting symptoms. Itis indicating that those typical symptoms on papaya is associated with Begomovirus infection, while the causal of leafmalformation and stunting is unknown yet. This work is the first report of Begomovirus infected papaya in Indonesia. Severedisease incidence caused by this pathogen was observed on papaya plants in Bengkulu Province that was in the range of 42–100%. This finding is a precious information to be used for identification, and characterization the species of the virus,determining control strategies against the disease.

Deteksi Begomovirus Pada Benih Pepaya Dan Pengendaliannya Dengan Metode Hot Water Treatment

“…Hasil deteksi menunjukkan pada sampel daun berhasil diamplifikasi pita DNA sebesar 900 pb. Sutrawati et al, (2021) Gambar 2. Visualisasi amplifikasi Begomovirus dari sampel daun papaya menggunakan primer universal SPG1 dan SPG 2 pada gel agarose 1% marker 1 kb, 900 pb DNA ladder (Thermo Scientific, AS); Ket: M sebagai marker 1 kb, (1-4) sampel benih berumur 7 hari, (5-8) sampel benih berumur 14 hari.…”

Begomovirus detection in the whitefly Bemisia spp. on eggplant Solanum melongena L. leaves

“…Detection of Begomovirus in plant leaves through the polymerase chain reaction (PCR) method has been carried out on eggplant, melon, chili, watermelon, papaya, and pepper plants [2,8]. DNA extraction is an initial step in Begomovirus detection using PCR.…”

Begomovirus Associated with Yellow Mosaic Symptom on Eggplant Plant in Bengkulu

“…Begomovirus telah dilaporkan keberadaannya di Indonesia pada tanaman tembakau, cabai, tomat, timun (Septariani et al 2014;Listihani et al 2019), melon (Subiastuti et al 2019), kedelai (Rahim et al 2015;Sutrawati et al 2020), terung, buncis, kacang panjang, dan gulma babadotan (Gaswanto et al 2016). Begomovirus yang pernah dilaporkan keberadaannya di Bengkulu antara lain pada tanaman pepaya dan mentimun (Tomato leaf curl New Delhi virus) (Sutrawati et al 2021). Begomovirus merupakan salah satu virus penting dan telah lama menjadi kendala utama dalam budi daya tanaman di daerah tropis dan subtropik di seluruh dunia (Rojas et al 2018).…”