First report of bleeding canker caused by <i>Rahnella</i> sp. on <i>Populus nigra</i> in Iran

Moradi-Amirabad, Yousef; Khodakaramian, Gholam

doi:10.5197/j.2044-0588.2020.041.037

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…These eight species contributed to the existing diversity of Rahnella with isolations of Rahnella victoriana, Rahnella variigena and Rahnella inusitata from bleeding cankers of oak; R. victoriana, R. variigena and R. woolbedingensis from asymptomatic alder and walnut; Rahnella bruchi from the gut of the Agrilus biguttatus beetle; Rahnella aceris and Rahnella laticis from sap of Acer pictum and Rahnella contaminans as a contaminant from MRSA agar plates [5][6][7]. In addition to their isolation from the natural environment, Rahnella species have been linked to nitrogen-fixation [8], metal and radionuclide sequestration [9] and biological control [10]; and more recently as possible pathogens of oak [11], poplar [12] and onion [13].…”

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confidence: 99%

Rahnella perminowiae sp. nov., Rahnella bonaserana sp. nov., Rahnella rivi sp. nov. and Rahnella ecdela sp. nov., isolated from diverse environmental sources, and emended description of the genus Rahnella

Brady

Asselin

Beer

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Bacteria isolated from onion bulbs suffering from bacterial decay in the United States and Norway were previously shown to belong to the genus Rahnella based on partial housekeeping gene sequences and/or fatty acid analysis. However, many strains could not be assigned to any existing Rahnella species. Additionally, strains isolated from creek water and oak as well as a strain with bioremediation properties were assigned to Rahnella based on partial housekeeping gene sequences. The taxonomic status of these 21 strains was investigated using multilocus sequence analysis, whole genome analyses, phenotypic assays and fatty acid analysis. Phylogenetic and phylogenomic analyses separated the strains into five clusters, one of which corresponded to Rahnella aceris . The remaining four clusters could be differentiated both genotypically and phenotypically from each other and existing Rahnella species. Based on these results, we propose the description of four novel species: Rahnella perminowiae sp. nov. (type strain SL6T=LMG 32257T=DSM 112609T), Rahnella bonaserana sp. nov. (H11bT=LMG 32256T=DSM 112610T), Rahnella rivi sp. nov. (FC061912-KT=LMG 32259T=DSM 112611T) and Rahnella ecdela sp. nov. (FRB 231T=LMG 32255T=DSM 112612T).

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mentioning

confidence: 99%

Rahnella perminowiae sp. nov., Rahnella bonaserana sp. nov., Rahnella rivi sp. nov. and Rahnella ecdela sp. nov., isolated from diverse environmental sources, and emended description of the genus Rahnella

Brady

Asselin

Beer

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…Decline is described as decreasing health and vigor of forest trees incited by abiotic and biotic agents (Helms, 1998). Forest tree declines characterized by trunk bark necrosis and bleeding of a dark sticky fluid from the vertical cracks developed on the trunks have been increasing in prevalence and severity in the Alborz and Zagros forests of oak, walnut, and alder in Iran (Ahmadi et al, 2019;Bakhshi Ganje et al, 2020;Moradi-Amirabad and Khodakaramian, 2020;Allahverdipour et al, 2021;Bakhshi Ganje et al, 2021). In the present study, we characterized strains of B. goodwinii associated with oak and oriental beech trees; the latter was identified for the first time as a new host species impacted by B. goodwinii.…”

Section: Discussionmentioning

confidence: 84%

Reliable and specific detection and identification of Brenneria goodwinii, the causal agent of oak and oriental beech decline

Araeinejhad,

Charkhabi,

Brady

et al. 2024

Front. For. Glob. Change

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Chestnut-leaved oak (Quercus castaneifolia) and oriental beech (Fagus orientalis) are among the major tree species in the Hyrcanian forests. Brenneria goodwinii was identified as the causal agent of necrotic lesions and stem bleeding on affected oak trees in different countries. Oak and oriental beech trees with bleeding symptoms were observed in a few forest sites in northern Iran. The objectives of the present study were to identify and characterize the causal agents of bark canker in oak and oriental beech trees and develop a primer set for specific detection, using polymerase chain reaction (PCR), of Brenneria goodwinii strains. A total of 31 and 20 samples from oak and oriental beech trees, respectively, with stem bleeding and bark canker symptoms were collected from Golestan and Mazandaran forests in northern Iran in 2020–2021. Bacterial strains displaying a green metallic sheen on EMB-agar medium were isolated from symptomatic oak (105 strains) and oriental beech samples (32 strains), while 31 and 20 strains were also isolated from healthy oak and oriental beech, respectively. Pathogenicity tests indicated that 51 and 25 strains isolated from oak and oriental beech, respectively were able to induce a necrotic area on oak acorns 15 days following inoculation. Moreover, four and two representative strains inoculated on oak and oriental beech twigs, respectively induced necrosis on all inoculated green twigs 1 month after inoculation. The sequences of the 16S rRNA and gyrB genes of representative strains isolated from and proved pathogenic on oak and oriental beech trees were 100% and over 99% similar to B. goodwinii LMG 26270T, respectively, which revealed the strains belong to B. goodwinii species. The primer pair BgF3/R2, which was designed to target the hrpN gene, was proven to be specific in the detection of B. goodwinii strains. The primer pair amplified a 618-bp DNA fragment from strains of B. goodwinii only and not from strains belonging to Rahnella, Gibbsiella, Lonsdalea, and the other Brenneria species among several other pathogenic bacteria tested. No fragment was amplified from DNA extracted from healthy trees or seedlings in PCR using this primer pair.

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“…victoriana was found associated with sudden decline of Persian oak ( Quercus brantii L.), chestnut‐leaved oak ( Quercus castaneifolia C.A. Mey) and hornbeam ( Carpinus betulus L.) (Moradi‐Amirabad et al, 2019), and Rahnella strains distinct from other named species were recently described as the causal agent of bleeding canker of black poplar ( Populus nigra L.) (Moradi‐Amirabad & Khodakaramian, 2020). In this study, the results of phenotypic and genetic analyses, and pathogenicity tests indicated that the isolated strains from diseased eucalypt trees, that were classified as R .…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of Rahnella victoriana associated with bacterial canker of Eucalyptus in Iran

et al. 2022

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Eucalypts are cultivated in many regions of the world because of their amenability to intensive plantation forestry (environmental adaptability and plasticity of clones, fast growth, easiness of rooting and strong re‐sprouting after coppicing), quality of the timber for multiple uses and high economic performance. Symptomatic Eucalyptus camaldulensis trees were sampled at two sites in the Mazandaran province of Iran. Plants showed trunk bark cracking and cankers accompanied by dark‐brown watery exudation from damaged tissues. Isolation, purification along with phenotypic and genotypic characterization of isolates were performed using routine bacteriological methods. Finally, the bacterial isolates obtained from cankers on symptomatic eucalyptus plants were identified as Rahnella victoriana based on results of biochemical tests in combination with analyses of partial sequences of the 16S rRNA, gyrB,rpoB, and atpD gene regions. When representative isolates were inoculated into the stem of 1‐year‐old E. camaldulensis saplings, necrotic lesions were visible in external and internal bark tissues 30 days after inoculation. The bacteria were re‐isolated from lesions up to 2 months post‐inoculation. This is the first report of bacterial canker of Eucalyptus caused by R. victoriana in Iran and in the world.

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First report of bleeding canker caused by Rahnella sp. on Populus nigra in Iran

Cited by 4 publications

References 5 publications

Rahnella perminowiae sp. nov., Rahnella bonaserana sp. nov., Rahnella rivi sp. nov. and Rahnella ecdela sp. nov., isolated from diverse environmental sources, and emended description of the genus Rahnella

Rahnella perminowiae sp. nov., Rahnella bonaserana sp. nov., Rahnella rivi sp. nov. and Rahnella ecdela sp. nov., isolated from diverse environmental sources, and emended description of the genus Rahnella

Reliable and specific detection and identification of Brenneria goodwinii, the causal agent of oak and oriental beech decline

Isolation and identification of Rahnella victoriana associated with bacterial canker of Eucalyptus in Iran

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