First Report of Chromosome Analysis of Two Dragonets (Perciformes, Callionymidae)

Abstract

We report the first chromosome analysis in the ocellated dragonet (Synchiropus ocellatus) and picturesque dragonet (S. picturatus) from Thailand. Kidney cell samples were taken from five male and five female fishes. The mitotic chromosome preparation was prepared directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of S. ocellatus and S. picturatus were 2n=40, and the fundamental number (NF) was 40 in both males and females. The chromosomes were present as large telocentric, medium telocentric and small telocentric chromosomes in numbers of 16-18-6 and 14-24-2, respectively. No morphological difference was identified between sex chromosomes of male and female specimens. After Ag-NOR banding technique, a single pair of nucleolar organizer regions (NORs) was observed on the long arm centromeric region of large telocentric chromosome pair 5 in S. ocellatus and chromosome pair 4 in S. picturatus. The karyotype formulas could be deduced as:

First Cytogenetic Study of Green Chromis, Chromis viridis (Perciformes, Pomacentridae) by Conventional Staining and Ag-NOR Banding Techniques

“…The cell suspensions were then fixed in Carnoy's fixative solution (ratio 3 methanol: 1 acetic acid). The cell suspensions were dropped on the slide (Getlekha et al, 2017;Kasiroek et al, 2017;Supiwong et al, 2017).…”

History and New Developments on the 90th Anniversary of Cytologia: On the Occasion of the Publication of “Dawn of Modern Wheat Genetics”

“…In Thailand, including Indochina and the northern Malay peninsula, Alongklod Tanomtong and coworkers have recently focused on fish karyotype analysis and have published 15 reports in Cytologia over the last 2 years on the following fish species: Humpback cardinalfish, Fibramia lateralis (Kasiroek et al 2017a); Banggai cardinalfish, Pterapogon kauderni (Kasiroek et al 2017b); triangle butterflyfish, Chaetodon triangulum and yellow butterflyfish, C. andamanensis (Supiwong et al 2017a); Indian vagabond butterflyfish, Chaetodon decussatus and Lined butterflyfish, C. lineolatus (Supiwong et al 2017b); Nile tilapia, Oreochromis niloticus (Sriuttha et al 2017); three-spot damselfish, Dascyllus trimaculatus (Getlekha et al 2017); ocellated dragonet, Synchiropus ocellatus and picturesque dragonet, S. picturatus (Kasiroek et al 2017c); crystal eye catfish, Hemibagrus wyckii (Supiwong et al 2017d); Chevey s sheetfish, Micronema cheveyi (Pinthong et al 2017); F 1 hybrid catfish: Mekong giant catfish, Pangasianodon gigas striped catfish, P. hypophthalmus and spot pangasius, Pangasius larnaudii Pangasianodon hypophthalmus (Sreeputhorn et al 2017); whitecheek monocle bream, Scolopsis vosmeri (Supiwong et al 2017c); snakehead fishes, Channa marulius and C. marulioides (Sarasan et al 2018); Hihgfin barb fish, Cyclocheilichthys armatus (Chaiyasan et al 2018); black lancer, Bagrichthys majusculus (Supiwong et al 2018); and longfin carp, Labiobarbus leptocheilus (Saenjundaeng et al 2018).…”

Karyotype and Chromosome Behavior Analyses in Three Regions of the Indomalayan Realm

“…He and his coworkers have published 92 articles, reporting the karyotypes of at least 124 species in Cytologia since his first such work in 2005. At least 32 of these karyotypes were the first to be reported worldwide, including those of Fibramia lateralis (Kasiroek et al 2017a), Pterapogon kauderni (Kasiroek et al 2017b), Chaetodon decussatus, C. lineolatus (Supiwong et al 2017a), C. triangulum, C. andamanensis (Supiwong et al 2017b), Synchiropus ocellatus, S. picturatus (Kasiroek et al 2017c), Axis axis (Khongcharoensuk et al 2017), Plectorhinchus pictus (Jantarat et al 2017a), Micronema cheveyi (Pinthong et al 2017b), Scolopsis vosmeri (Patawang et al 2017d), and Crassostrea iredalei (Chooseangjaew et al 2018). Professor Tanomtong won the second Wada Memorial Award for this achievement.…”