First report of Cucumber mosaic virus infecting Ocimum gratissimum (L.) in Calabar, Cross River State, Nigeria

Ekpiken, E. E.; Eyong, O. I.; Mofunanya, A. A. J.; Owolabi, A. T.; Arogundade, Olawale

doi:10.1007/s13337-021-00662-x

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…However, they were clustered to the IB subgroup. Previously characterised CMV isolates, especially from Nigeria, have been placed in either subgroups IA, IB or II (Ayo-John and Hughes, 2014; Kayode et al, 2014;Ekpiken et al, 2021). The occurrence of subgroup IB in this study, and its prevalence in Nigeria, strongly supports an earlier report by Haase et al (1989) which suggested that subgroup I isolates were more commonly found in the tropics and subtropics while subgroup II isolates were prevalent in the temperate regions.…”

Section: Analysis Of Sequencessupporting

confidence: 84%

“…Table 1 and Figure 4 showed that the 31 isolates, including those retrieved from GenBank, were divided into four main phylogroups inconsistent with geographic origins and hosts, with each phylogroup sub- (Adediji, 2019;Arogundade et al, 2019;Eyong et al, 2020;Ekpiken et al, 2021;Apalowo et al, 2022;Longe et al, 2022). The present investigation revealed the natural occurrence of CMV on P. foetida with its detection and identification based on ELISA testing and sequencing of its partial genome.…”

Section: Analysis Of Sequencesmentioning

confidence: 99%

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Causal agent for disease symptoms on passionflower in Adim, Cross River State, Nigeria

Ekpiken,

Eyong,

Adediji

2024

Afr. Crop Sci. J.

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The stinking passionflower (Passiflora foetida L.) is generally regarded as a weed, although its tender fruits are consumed by a few; while the plant is mostly regarded for its medicinal properties. Cucumber mosaic virus is known not just to cause significant losses among crops, but also present in many weeds species. The objective of this study was to identify the causal agent of the disease symptoms observed from P. foetida in Adim, Cross River State, Nigeria. Leaves of P. foetida with virus symptoms were collected from various locations in Adim. Antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and reverse transcription polymerase chain reactions (RT-PCR) were used for virus detection and characterisation. The symptomatic leaves reacted positively with the CMV antisera in ACP-ELISA and sequences from RT-PCR products showed the highest identity (98.5%) with a CMV isolate from Xanthosoma sp. (MG021460) in Uganda. Phylogenetic reconstruction aggregated the isolates with other Nigerian CMV sequences in a separate uncharacterised subgroup, closest to MH798809 (Nigeria), MW655577 (Nigeria) and HQ874434 (Austria). The tree also revealed the isolates as members of subgroup IB. This study provides the first molecular evidence of CMV infection on P. foetida in Nigeria and adds to the list of possible natural host for the virus in the country.

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Section: Analysis Of Sequencessupporting

confidence: 84%

Section: Analysis Of Sequencesmentioning

confidence: 99%

Causal agent for disease symptoms on passionflower in Adim, Cross River State, Nigeria

Ekpiken,

Eyong,

Adediji

2024

Afr. Crop Sci. J.

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“…For example, CMV infects plant species belonging to different botanical families, such as Brassicaceae , Solanaceae , Papilionaceae , and Cucurbitaceae , including weeds and ornamental plants such as Chenopodium amaranticolor , Datura innoxia , Hibiscus rosa or Salvia splendens [ 49 , 50 , 51 ]. Recently, it has also been reported to infect Ocimum gratissimum L. in Nigeria [ 52 ] and Tylophora indica in India [ 53 ]. In Spain, CMV has been found to be widely distributed in cucumber, melon, pumpkin, zucchini, watermelon, tomato, pepper, aubergine, tobacco, beans, celery, and borage horticultural crops, among others [ 42 , 47 , 50 , 54 ].…”

Section: Description Of the Main Aphid-transmitted Virus In Cucurbitsmentioning

confidence: 99%

Occurrence, Distribution, and Management of Aphid-Transmitted Viruses in Cucurbits in Spain

2023

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The effectiveness of pest and disease management in crops relies on knowledge about their presence and distribution in crop-producing areas. Aphids and whiteflies are among the main threats to vegetable crops since these hemipterans feed on plants, causing severe damage, and are also able to transmit a large number of devastating plant viral diseases. In particular, the widespread occurrence of aphid-transmitted viruses in cucurbit crops, along with the lack of effective control measures, makes surveillance programs and virus epidemiology necessary for providing sound advice and further integration into the management strategies that can ensure sustainable food production. This review describes the current presence and distribution of aphid-transmitted viruses in cucurbits in Spain, providing valuable epidemiological information, including symptom expressions of virus-infected plants for further surveillance and viral detection. We also provide an overview of the current measures for virus infection prevention and control strategies in cucurbits and indicate the need for further research and innovative strategies against aphid pests and their associated viral diseases.

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Genetic Structure of Cucumber Mosaic Virus From Natural Hosts in Nigeria Reveals High Diversity and Occurrence of Putative Novel Recombinant Strains

et al. 2022

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Cucumber mosaic virus (CMV, Bromoviridae: Cucummovirus), one of the most widespread plant viruses with several hosts, causes huge losses in yield quality and quantity. The occurrence of various CMV strains and high genetic diversity within the virus complicate its management. We describe the population structure of CMV in Nigeria using partial RNA1 and RNA3 gene sequences from three natural hosts: pepper (Capsicum annuum), tomato (Solanum lycopersicum), and watermelon (Citrullus lanatus). One hundred and six leaf samples were obtained from 16 locations across Nigeria, and specific primers were used to amplify the two gene fragments using PCR. Twenty-four samples tested positive for CMV using RNA1 primers, and amplicons were sequenced from 12 isolates, revealing 82.94–99.80% nucleotide and 85.42–100% amino acid sequence similarities within the population. The partial RNA3 fragment, corresponding to the complete coat protein (CP) gene, was sequenced from seven isolates, with 95.79–97.90% and 98.62–100% nucleotide and amino acid intrapopulation similarities, respectively. The isolates belonged to subgroup IB and formed distinct phylogenetic clusters in both gene sets, indicating putative novel strains. Recombination signals, supported by phylogenetic inferences, were detected within the RNA1 dataset (P ≤ 0.05) and identified a recombinant isolate within the Nigerian sequences. No recombination was detected within the CP genes. Population genetics parameters established high diversity within the Nigerian population compared to other isolates worldwide, while selection pressure estimates revealed the existence of negative selection in both gene sets. Although CMV subgroup IB strains were postulated to originate from Asia, this study reveals their prevalence across several hosts from different locations in Nigeria. To our knowledge, this is the first comprehensive description of a recombinant CMV subgroup IB isolate from West Africa, which has implications for its robust detection and overall management.

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First report of Cucumber mosaic virus infecting Ocimum gratissimum (L.) in Calabar, Cross River State, Nigeria

Cited by 3 publications

References 4 publications

Causal agent for disease symptoms on passionflower in Adim, Cross River State, Nigeria

Causal agent for disease symptoms on passionflower in Adim, Cross River State, Nigeria

Occurrence, Distribution, and Management of Aphid-Transmitted Viruses in Cucurbits in Spain

Genetic Structure of Cucumber Mosaic Virus From Natural Hosts in Nigeria Reveals High Diversity and Occurrence of Putative Novel Recombinant Strains

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