2018
DOI: 10.1016/j.sjbs.2018.01.006
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First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon)

Abstract: Black cutworm (BCW) , an economically important lepidopteran insect, has attracted a great attention. (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact, bio-pesticide formulations and insect resistant transgenic plants based on the bacterium Bt delta-endotoxin have attracted worldwide attention as a safer alternative to harmful chemical pesticides. The major objective of the current study was to unders… Show more

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Cited by 8 publications
(11 citation statements)
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“…This demonstrated the selective precipitation of the ∼48 kDa protein from the solubilized BBMV of BCW along with Vip3A toxin. Previously, it has been demonstrated by radio-labeled blotting using 125 I-Vip3A that Vip3A toxin binds to the same ∼48 kDa protein from the BBMV of BCW (Osman et al, 2017); this suggests that the precipitated protein formed a complex with Vip3A toxin, anti-Vip3A is the only binding protein for Vip3A, and it is the same binding protein that is found on the epithelial cells of BCW (Fig. 1).…”
Section: Resultsmentioning
confidence: 68%
See 1 more Smart Citation
“…This demonstrated the selective precipitation of the ∼48 kDa protein from the solubilized BBMV of BCW along with Vip3A toxin. Previously, it has been demonstrated by radio-labeled blotting using 125 I-Vip3A that Vip3A toxin binds to the same ∼48 kDa protein from the BBMV of BCW (Osman et al, 2017); this suggests that the precipitated protein formed a complex with Vip3A toxin, anti-Vip3A is the only binding protein for Vip3A, and it is the same binding protein that is found on the epithelial cells of BCW (Fig. 1).…”
Section: Resultsmentioning
confidence: 68%
“…It has also been concluded that several factors may influence the insect host range of the insecticidal proteins produced by Bt subspecies, which include: (i) solubilization of the crystal protein, (ii) processing by midgut proteases, (iii) presence of specific binding sites on the susceptible cells, and (iv) insertion of the bound toxin into the membrane (Vadlamudi et al, 1993, Vadlamudi et al, 1995). In a recent study, several proteins of size 48, 40, and 31 kDa from the BBMV of Agrotis ipsilon (BCW) were identified and purified (Osman et al, 2017). Immunoprecipitation with the same antiserum and subsequent ligand blotting with Vip3A toxin demonstrated binding of the toxin to the 48-kDa protein from the BBMV of BCW midgut.…”
Section: Discussionmentioning
confidence: 99%
“…This proteolytic processing has been shown to be required for Vip3 cytotoxicity 10 , 11 . In the midgut of susceptible larvae, the toxin binds to receptors on target cells that are not yet fully characterised 12 , 13 . At some stage following receptor engagement, the Vip3 proteins are proposed to form pores in insect cell membranes 10 .…”
Section: Introductionmentioning
confidence: 99%
“…The remaining food, dead larvae and residues were isolated, the larvae were then placed on a natural food environment under the previously established laboratory conditions. Four replicates were made for each concentration and all larvae were left to complete their life cycle after calculating the number of dead larvae 16 .…”
Section: Preparation Of Bacterial Suspensionmentioning
confidence: 99%