First report of Giardia duodenalis genotypes in Zangxiang pigs from China

Zhang, H. J.; Song, Jun-Ke; Wu, Xuemei; Li, Y. H.; Wang, Y.; Lin, Qing; Zhao, Guanghui

doi:10.1007/s00436-019-06340-8

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Two novel subtypes (named as E45*, n = 4 and E46*, n = 1) and 3 novel subtypes (named as E42*, n= 5; E43*, n= 2 and E44*, n= 1) of assemblage E were identified at the gdh and tpi loci, respectively (Table 2). Previous studies have shown that the assemblage E is the primary genotype in different animals [1,[39][40][41][42][43][44][45]. Both assemblage A and assemblage E were also found in humans [1,5].…”

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confidence: 91%

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified

Zou

Zhang

et al. 2020

Korean J Parasitol

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Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

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confidence: 91%

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified

Zou

Zhang

et al. 2020

Korean J Parasitol

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“…In Xinjiang, China, detection rates of G. duodenalis in pigs through PCR amplification of SSU rRNA were lower (2.6%), and genotyping showed a predominance of assemblage B, but also the presence of some infections with assemblage A and E (Jing et al 2019). In the Chinese provinces of Shaanxi and Qinghai, the positivity rate by PCR was 6.2%, with the predominance of assemblage E and some isolates characterized as assemblage B (Zhang et al 2019). In Tibet, the detection of G. duodenalis in fecal specimens from pigs by PCR (GDH loci) revealed a positivity of 0.58% and the presence of assemblages D and E (Zou et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Detection and genotyping of Giardia duodenalis infecting pigs and small ruminants in the state of Piauí, northeastern Brazil

Calegar,

Coronato-Nunes,

Bacelar

et al. 2023

Pesq. Vet. Bras.

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This study performed a molecular detection and characterization of Giardia duodenalis infecting pigs, goats and sheep in rural and peri-urban communities in the state of Piauí, northeastern Brazil, and proposed phylogenetic relationships among the characterized parasites. We assessed 52 fecal samples from pigs, 13 from goats, and 10 from sheep. A fragment of the β-giardin locus was PCR-amplified and sequenced. Overall, PCR-based G. duodenalis positivity was 11/52 (21.2%) in pigs, 2/13 (15.4%) in goats, and 2/10 (20%) in sheep. Seven out of 15 successfully amplified samples could be sequenced: three from pigs, two from goats, and two from sheep. Parasites from different hosts were found to belong to sub-assemblage AII. The phylogenetic analyses of the original G. duodenalis AII β-giardin sequences obtained from distinct host species and sequences of G. duodenalis recovered from humans available in GenBank suggest that the parasites are genetically related, supporting a local scenario of cross-host transmission.

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“…Human infection occurs through the digestive tract by consumption of food or water contaminated with the faeces of infected animals containing Cryptosporidium oocysts or Giardia cysts [15]. The prevalence of Cryptosporidium parvum in dairy calves has been confirmed in Argentina [16] and in Germany [15], and Giardia duodenalis among livestock and pets in Poland [17], and in pigs in China [18]. Therefore, the foodborne transmission of both parasites may be more common than until recently recognized.…”

Section: Introductionmentioning

confidence: 99%

Potential sources of infection with selected zoonotic agents in the veterinary work environment – pilot studies

Wójcik-Fatla

Sroka

Zając

et al. 2020

Ann Agric Environ Med.

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Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%)-Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.

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First report of Giardia duodenalis genotypes in Zangxiang pigs from China

Cited by 5 publications

References 41 publications

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified

Detection and genotyping of Giardia duodenalis infecting pigs and small ruminants in the state of Piauí, northeastern Brazil

Potential sources of infection with selected zoonotic agents in the veterinary work environment – pilot studies

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