First report of Giardia duodenalis assemblage F in humans and dogs in southern Brazil

Silva, Ana Clécia dos Santos; Martins, Felippe Danyel Cardoso; Ladeia, Winni Alves; Kakimori, Monica Tiemi Aline; Lucas, Juliana Izidoro; Sasse, João Pedro; Martins, Thais Agostinho; Macedo, Vilma de Fátima Rodrigues de; Murad, Valter Abou; Barros, Luiz Daniel de; Garcia, João Luís

doi:10.1016/j.cimid.2022.101878

Cited by 8 publications

(6 citation statements)

References 55 publications

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“…However just as the use of only one parasitological technique underestimated the prevalence of giardiasis in our study, the individual use of PCR can also underestimate cases in human and animal populations (Arroyo-Salgado et al, 2014;David et al, 2015). It should also be noted that the PCR technique can have varied sensitivity because, in addition to the presence of inhibitors, the extraction of DNA from the cysts is variable, with amplification rates from 36% to 85.7% (Babaei et al, 2011;Silva et al, 2022). Prevalence and diagnostic efficiency of molecular methods depend on the type and number of loci analyzed, and their applicability has been mainly restricted to the epidemiological purpose due to their high cost of execution (Feng & Xiao, 2011;Ryan & Cacciò, 2013).…”

Section: Discussionmentioning

confidence: 63%

Giardiasis in children and dogs, and the first report of assemblage E in dogs from northeastern Brazil

Harvey

Carvalho

Aquino

et al. 2023

Rev. Bras. Parasitol. Vet.

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Diagnosis is crucial for controlling giardiasis. We determined the prevalence and genetically characterize isolates of Giardia duodenalis of children and dogs from rural communities in northeastern Brazil. G. duodenalis cysts were concentrated by centrifugal flotation/sedimentation. Molecular characterization was carried out using the loci ssu-rRNA, bg, tpi, and gdh. By parasitological techniques, Giardia spp. infection was detected in 72/192 children (37.5%; 95% CI: 30.6%-44.7%) and 24/139 dogs (17.3%; 95% CI: 11.4%-24.6%). By molecular analysis, infection was detected in 60/141 children (42.5%; 95% CI: 34.3%-51.2%) and 26/92 dogs (28.3%; 95% CI: 19.4%-38.6%). The total prevalence of giardiasis was 54.9% in children (106/193; 95% CI: 47.1%-61.6%) and 32.9% in dogs (47/143; 95% CI: 25.2%-41.2%). Zoonotic assemblages A and B of G. duodenalis were detected in children, and assemblage E of G. duodenalis was detected in one child and two dogs. Parallel use of parasitological and molecular techniques proved to be a more effective strategy for detecting giardiasis in children and dogs from endemic areas.

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Section: Discussionmentioning

confidence: 63%

Giardiasis in children and dogs, and the first report of assemblage E in dogs from northeastern Brazil

Harvey

Carvalho

Aquino

et al. 2023

Rev. Bras. Parasitol. Vet.

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“…These authors emphasize that more studies are necessary to ascertain the extent of the zoonotic transmission of Giardia since the cross-transmission cannot be proved just by the detection of the same assemblages in animal companions and humans. However, the zoonotic potential of this protozoan cannot be underestimated (Silva et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of gastrointestinal parasites in dogs in a rural area of Santa Catarina, Brazil

Arruda,

Bresciani,

Werner

et al. 2023

Rev. Bras. Parasitol. Vet.

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We investigated the occurrence of gastrointestinal parasites in dogs in rural areas of the municipality of Painel, Santa Catarina, Brazil. For this, 91 canine feces samples were collected from 82 farms between August, 2017 and January, 2018. These fecal materials were processed using the techniques of spontaneous sedimentation, centrifugation-floatation in zinc sulfate and Ziehl-Neelsen staining. A questionnaire in the form of an interview was administered to the dogs’ owner and information about the farm and the main care provided for the dogs was obtained. Among 91 sampled dogs, 27 (29.7%) were positive for at least one parasite species. Ancylostoma was the most prevalent genus (16.5%), followed by Giardia duodenalis (14.3%), Trichuris vulpis (6.6%), Toxocara canis (5.5%), Entamoeba spp. (4.8%), Cryptosporidium spp. (3.3%) and Taeniidae (1.1%). Most dogs do not receive veterinarian care and rarely received antiparasitic treatment. They were free to roam and had free access to animal remains and garbage, which was reflected in the significant associations with the occurrence of parasites that were found. We conclude that rural dogs harbor gastrointestinal parasites, but that their owners are unaware of the risks that these parasites can bring to human health.

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“…In one study in Brazil, assemblage E was the only assemblage detected in dogs, and assemblages A, B and E were recovered from children from the same rural community ( Harvey et al, 2023 ). Another study of Giardia -positive samples from humans, dogs and cats in Brazil, identified assemblage F in one human and assemblages C, D and F in dogs and F in one cat ( Silva et al, 2022 ). These studies are suggestive of zoonotic transmission but have not been confirmed by finding identical multilocus subtypes in both humans and their companion animals.…”

Section: Zoonotic Risk Of Cryptosporidiosis and Giardiasis From Cats ...mentioning

confidence: 99%

Cryptosporidium and Giardia in cats and dogs: What is the real zoonotic risk?

Barbosa,

Egan,

Feng

et al. 2023

Current Research in Parasitology & Vector-Borne Diseases

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First report of Giardia duodenalis assemblage F in humans and dogs in southern Brazil

Cited by 8 publications

References 55 publications

Giardiasis in children and dogs, and the first report of assemblage E in dogs from northeastern Brazil

Giardiasis in children and dogs, and the first report of assemblage E in dogs from northeastern Brazil

Occurrence of gastrointestinal parasites in dogs in a rural area of Santa Catarina, Brazil

Cryptosporidium and Giardia in cats and dogs: What is the real zoonotic risk?

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