First Report of Grapevine Pinot gris Virus on Grapevines in Armenia

Eichmeier, Aleš; Peňázová, Eliška; Nebish, A.

doi:10.1094/pdis-09-19-1944-pdn

Cited by 15 publications

(12 citation statements)

References 10 publications

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“…Since the discovery of GPGV (Trichovirus) [16], the virus has been shown to be widespread in vineyards around the world, including in France [68], USA [69], Republic of Korea [70], Turkey [71], China [72], Canada [73], Germany [74], Pakistan [75], Spain [76], Brazil [77], Chile [78], Lebanon [79], Moldova [80], Georgia [81], Iran [82], Australia [83], Czech Republic [84], Algeria [85], England [86], Armenia [87], Bulgaria [88], Argentina [89], California [90], Japan [91], Belgium [92], Romania [93], Hungary [21], and Russia [47]. In 89% (34 out of 38) of the samples, we detected GPGV.…”

Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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The use of high-throughput sequencing (HTS) technology has led to significant progress in the identification of many viruses and their genetic variants. In this study, we used the HTS platform to sequence small RNAs (sRNAs) of grapevine to study the virome. Isolation of RNA was performed using symptomatic grapevines collected from commercial vineyards in Krasnodar Krai in 2017–2018. To determine the viromes of vineyards, we used an integrated approach that included a bioinformatic analysis of the results of sRNA HTS and the molecular method RT-PCR, which made it possible to identify 13 viruses and 4 viroids. Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine Syrah Virus-1 (GSyV-1), Raspberry bushy dwarf virus (RBDV), Australian grapevine viroid (AGVd), and Grapevine yellow speckle viroid 2 (GYSVd-2) were identified for the first time in Russia. Out of 38 samples analyzed, 37 had mixed infections with 4–11 viruses, indicating a high viral load. Analysis of the obtained sequences of fragments of virus genomes made it possible to identify recombination events in GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4, GVT, GPGV, GRSPaV, GVA, and GFLV. The obtained results indicate a wide spread of the viruses and a high genetic diversity in the vineyards of Krasnodar Krai and emphasize the urgent need to develop and implement long-term strategies for the control of viral grapevine diseases.

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Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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“…The phylogenetic study demonstrated that the Algerian GPGV isolates were genetically similar. The geographic uniqueness of GPGV isolates was confirmed by Eichmeier et al [ 3 , 5 , 10 , 24 ]. Given the similarities between GLMD and boron deficiency symptoms in grapevines, Buoso et al [ 41 ] suggested that GPGV interferes in boron homeostasis.…”

Section: Discussionmentioning

confidence: 74%

“…Pinot gris in which it causes grapevine leaf mottling and deformation disease (GLMD) [ 1 , 2 ]. GPGV is widespread in many wine-producing countries around the world [ 3 ], and was more recently detected in Canada [ 4 ], Armenia [ 5 ], Australia [ 6 ], Brazil [ 7 ], Chile [ 8 ] and Pakistan [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The presence of sequence variations in GPGV isolates remains unknown worldwide. There have been a few studies on the variability of the GPGV genome, and they have suggested that the genome is genetically diverse and consists of numerous haplotypes [ 2 , 5 , 12 , 18 , 19 ]. Recently, Hily et al [ 16 ] published genetic diversity analyses of 100 new complete or near-complete GPGV genomic sequences.…”

Section: Introductionmentioning

confidence: 99%

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Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity

Eichmeier

Peňázová

Čechová

et al. 2020

Genes

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Grapevine Pinot gris virus (GPGV) is a putative causal agent of grapevine leaf mottling and deformation disease that has been reported worldwide throughout the grapevine-growing regions. Fifty-four grapevines collected from five Algerian grapevine-growing regions were tested for the presence of GPGV in phloem tissues. Eight of the tested grapevines were infected by GPGV. Viromes of two selected Vitis vinifera cv. Sabel grapevines infected by GPGV and showing virus-like symptoms were analyzed by small RNA sequencing. Phylogenetic analyses of the partial coding sequence (cds) of the RNA-dependent RNA polymerase (RdRp) domain showed that all Algerian GPGV isolates were grouped with some already-described asymptomatic isolates. This study provides the first survey of the occurrence of GPGV in Algeria. Moreover, Grapevine fleck virus, Grapevine rupestris stem pitting-associated virus, Grapevine virus B, Grapevine rupestris vein feathering virus, Hop stunt viroid and Grapevine yellow speckle viroid 1 were detected in Algeria for the first time.

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“…Pinot gris, in which it causes Grapevine Leaf Mottling and Deformation (GLMD) disease [1,2]. GPGV is widespread in many wine-producing countries around the world [3], and it was recently detected in Canada [4], Armenia [5], Australia [6], Brazil [7], Chile [8], Pakistan [9], and Algeria [10]. The typical symptoms, which are apparent mostly in the beginning of the season, are delayed budburst, leaf distortion and mottling, stunted shoots, and poor yield due to increased berry acidity [2].…”

Section: Introductionmentioning

confidence: 99%

Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes

et al. 2021

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Grapevine Pinot gris virus (GPGV) is considered to be a causal agent of Grapevine Leaf Mottling and Deformation (GLMD) disease that has been reported worldwide through the grapevine-growing regions. Seven grapevines that were collected from a vineyard in the Czech Republic were tested for the presence of GPGV in leaf and phloem tissues. Each of the seven grapevines was infected by GPGV, from which sic symptoms were mostly shown without a typical mottling. The phylogeny based on RNA-dependent RNA polymerase and movement/coat protein sequences indicated the same origin of the GPGV isolates. The GPGV titer was the highest in the grapevines with the highest GLMD-like symptoms; however, some of the grapevines with milder GLMD-like symptoms had a lower GPGV titer than the asymptomatic grapevine. Soil analysis showed uneven boron content in the direct vicinity of the grapevines, while the boron content in the grapevines was more, even showing no boron deficiency. The quantitative analysis of selected gene expressions associated with boron efflux and transport only partially explained the boron content in the soil and grapevines and only in the grapevines growing in soils with the highest or lowest boron contents. The VvBor2 and VvNIP5 genes had a higher expression and VvNIP6 had a lower expression in the grapevine growing in the soil with the lowest boron content, while a low expression of VvBor1 and VvBor2 was observed in the grapevine that was grown in the soil with the highest boron content.

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First Report of Grapevine Pinot gris Virus on Grapevines in Armenia

Cited by 15 publications

References 10 publications

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity

Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes

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