First report of gray mold rot disease on tomato (Solanum lycopersicum L.) caused by Botrytis cinerea in Malaysia

Tijjani, Abdulnasir; Ismail, Siti Izera; Ahmad, Khalil; Omar, Dzolkhifli

doi:10.1007/s42161-018-0155-2

Cited by 3 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification of this specific fungus was viewed under a microscope for morphology characteristics. The morphological and molecular characteristics of B. cinerea were similar to the description of Javed et al [20] and Tijjani et al [3].…”

Section: Preparation Of B Cinereasupporting

confidence: 84%

“…Subjected to scientific and economic importance, B. cinerea was ranked as the second top plant pathogen in the world [ 2 ]. In 2015, Tijjani et al [ 3 ] observed tomato fruits with gray mold symptoms in postharvest storage at Cameron Highlands with 65% disease incidence, and this is the first report of gray mold disease on tomato fruits in Malaysia.…”

Section: Introductionmentioning

confidence: 92%

“…Subjected to scientific and economic importance, B. cinerea was ranked as the second top plant pathogen in the world [2]. In 2015, Tijjani et al [3] voucher specimen (SK 3280/18) was deposited in the herbarium of the same institute. The plants were thoroughly cleaned, and the stems were cut into 25 cm lengths and planted in Ladang 15, Universiti Putra Malaysia (geographical coordinates: 2°59′19.8″ North, 101°43′50″ East, Malaysia) in December 2017.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

Self Cite

View full text Add to dashboard Cite

Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

show abstract

Section: Preparation Of B Cinereasupporting

confidence: 84%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…and humidity, are key to the promotion of grape gray mold outbreaks [ 4 , 13 , 14 , 15 , 16 ]. Considering the size of B. cinerea spores (10–12 × 8–10 μm), spore infection of the stoma in grape (23–28 × 15–18 μm) occurs easily [ 13 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

A Simulation Study to Reveal the Epidemiology and Aerosol Transmission Characteristics of Botrytis cinerea in Grape Greenhouses

Yuan,

Jiang,

et al. 2024

Pathogens

View full text Add to dashboard Cite

Most previously studies had considered that plant fungal disease spread widely and quickly by airborne fungi spore. However, little is known about the release dynamics, aerodynamic diameter, and pathogenicity threshold of fungi spore in air of the greenhouse environment. Grape gray mold is caused by Botrytis cinerea; the disease spreads in greenhouses by spores in the air and the spore attaches to the leaf and infects plant through the orifice. In this study, 120 μmol/L propidium monoazide (PMA) were suitable for treatment and quantitation viable spore by quantitative real-time PCR, with a limit detection of 8 spores/mL in spore suspension. In total, 93 strains of B. cinerea with high pathogenicity were isolated and identified from the air samples of grapevines greenhouses by a portable sampler. The particle size of B. cinerea aerosol ranged predominately from 0.65–3.3 μm, accounting for 71.77% of the total amount. The B. cinerea spore aerosols were infective to healthy grape plants, with the lowest concentration that could cause disease being 42 spores/m3. Botrytis cinerea spores collected form six greenhouse in Shandong Province were quantified by PMA-qPCR, with a higher concentration (1182.89 spores/m3) in May and June and a lower concentration in July and August (6.30 spores/m3). This study suggested that spore dispersal in aerosol is an important route for the epidemiology of plant fungal disease, and these data will contribute to the development of new strategies for the effective alleviation and control of plant diseases.

show abstract

Identification and characterization of Botrytis cinerea causing gray mold on tomato in Malaysia

Yusoff,

Ismail,

Haron

et al. 2024

MAB

View full text Add to dashboard Cite

Botrytis cinerea, commonly known as gray mold, is a pervasive fungal pathogen that affects a wide range of plant species, leading to significant agricultural losses. The identification of Botrytis cinerea in Malaysia is crucial for protecting the agricultural sector, minimizing economic losses, ensuring food security, maintaining export quality, addressing environmental concerns, and advancing scientific research. In the present research, tomato fruits collected from Cameron Highlands, Pahang, Malaysia showed gray mold disease symptoms of B. cinerea. The fungal isolates were examined morphologically for colony colour, growth rate, conidiophores, conidia shape, and sclerotia on PDA and V8 agar. According to the results, conidiophores appeared in grape shape and length was range of 21.26-32.52 μm, ovoid conidial dimensions were in the range of 10.03-16.08 × 7.37-11.15 μm and sclerotia size was range 1.91-4.50 × 1.70-4.00 mm. All isolates were attributed to the morphospecies Botrytis cinerea on account of these characteristics. The resulting sequences deposited in GenBank were accessions MT012053 to MT012062, respectively. A BLAST analysis of the resulting 550-bp nucleotide sequences showed 99-100% identity closest matched to B. cinerea. The pathogenicity experiments showed P6 isolates of B. cinerea were highly pathogenic and caused gray mold development on tomato fruits that led to severe symptoms in five days. Meanwhile, the least pathogenic isolate was P9. In terms of temperature, B. cinerea grew faster on PDA at 20ºC, slower grew below 20ºC and did not grow at 25ºC. Identification and characterization of B. cinerea on tomato could potentially provide information to assist disease management strategies for B. cinerea.

show abstract

First report of gray mold rot disease on tomato (Solanum lycopersicum L.) caused by Botrytis cinerea in Malaysia

Cited by 3 publications

References 2 publications

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

A Simulation Study to Reveal the Epidemiology and Aerosol Transmission Characteristics of Botrytis cinerea in Grape Greenhouses

Identification and characterization of Botrytis cinerea causing gray mold on tomato in Malaysia

Contact Info

Product

Resources

About