First Report of Alternaria alternata Causing Black Spot on Tomato (Solanum lycopersicum) in Tongzhou, China

Ren, Zhaoyu; Cao, Aocheng; Li, J.; Guo, M. X.; Wang, Q. X.; Yan, Dongdong; Ouyang, Canbin; Li, Y.

doi:10.1094/pdis-03-17-0346-pdn

Cited by 4 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, Adhikari et al [ 6 ] stated that annual losses in tomato yield caused by A. alternata and A. solani have been estimated at 79%. The small-spored Alternaria species, e.g., A. alternata , A. arborescens , A. tenuissima , A. dumosa , and A. interrupta , have been reported to cause diseases in Solanaceous hosts [ 7 , 8 , 9 ]. In addition to infecting fruits and vegetables, Alternaria species are also well-known sources of more than 70 toxins that can lead to many human health disorders [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats

Ismail

Elshewy

El-Ganainy

et al. 2023

JoF

View full text Add to dashboard Cite

The Alternaria species is considered to contain a plethora of several mycotoxins constituting a risk factor for both human and animal health. This work aimed mainly to explore the cytotoxicity of a combined mixture of altenuene (ALT), alternariol (AOH), tenuazonic acid (TeA), and altenuisol (AS) toxins produced by pathogenic A. alternata toward human oral epithelial cells (PCS-200-014), lung fibroblast cells (WI-38), and male albino rats. The sequencing of the multi-locus, RNA polymerase second largest subunit (rpb2), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and Alternaria major allergen gene (Alt a 1) was performed to infer relationships among isolated Alternaria species. The phylogenetic analysis of gapdh, rpb2, and Alt-a 1 sequence data indicated that all isolates resided in A. alternata. The pathogenic potentiality of A. alternata was investigated on tomato plants cv. super strain B under greenhouse conditions, and all isolates were pathogenic to tomato plants, with significant (p < 0.05) variations. The ability of A. alternata isolates to produce mycotoxins was also explored using high-performance liquid chromatography (HPLC). All tested isolates were able to produce at least one of the assessed mycotoxins—ALT, AOH, TeA, and AS—and ALT was reported as the dominant mycotoxin, produced by 80% of A. alternata isolates. The cytotoxic properties of the combined mixture of ALT, AOH, TeA, and AS at concentrations of 31.25, 62.50, 125, 250, and 500 µg/mL were assessed via the MTT assay method after exposure for 24 h versus the control. The treatment of both cell lines with combined mixtures of ALT, AOH, TeA, and AS showed a dose-dependent decrease in cell viability. The highest concentrations tested at 62.50, 125, 250, and 500 µg/mL significantly decreased cell viability and caused cell damage compared to the lowest concentration of 31.25 µg/mL and the control. The cytotoxicity and genotoxicity of the combined mixtures of ALT, AOH, TeA, and AS on male albino rats were also investigated via the gene expression of (TNF-α) and using hematological (CBC), chemical (alanine aminotransferase (ALT), aspartate aminotransferase (AST) and urea and creatinine), and histopathological analyses. A marked increase was observed in the levels of ALT, AST, urea and creatinine, TNF-α gene expression, red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), and packed cell volume % (PCV) after 28 days of exposure relative to the untreated control. Pathological alterations were also observed in the liver and kidney tissues of rats. Conclusively, this work provides a new understanding on the cytotoxicity and genotoxicity of mycotoxins of pathogenic A. alternata from tomatoes.

show abstract

Section: Introductionmentioning

confidence: 99%

Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats

Ismail

Elshewy

El-Ganainy

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…However, it has been shown that A. linariae, A. grandis and A. protenta can also infect both tomato (Solanum lycopersicum L.) and potato (Solanum tuberosum L.) [12][13][14][15]. Moreover, several small-spored species have been reported to cause necrotic lesions on Solanaceae, e.g., A. alternata, A. arborescens, A. tenuissima, A. dumosa, A. interrupta [8,14,[16][17][18][19]. However, the exact number of species responsible for Solanaceae leaf spot diseases should be taken with caution due to the recent redefinition of species within the genus Alternaria, based on multi-gene phylogeny.…”

Section: Introductionmentioning

confidence: 99%

Characterization of New Small-Spored Alternaria Species Isolated from Solanaceae in Algeria

Bessadat

Hamon

Bataillé‐Simoneau

et al. 2021

Life

View full text Add to dashboard Cite

Although large-spored Alternaria species of the section Porri are considered to be the major agents responsible for leaf spot and blight of Solanaceae, small-spored Alternaria species are also frequently isolated from symptomatic tissues. A survey of the north-western regions of Algeria during the 2017–2018 growing seasons revealed that amongst the 623 Alternaria isolates from tomato, potato, pepper, eggplant and black nightshade, 8% could not be morphologically assigned to either section Porri or section Alternaria. In order to more precisely determine the taxonomic position of these isolates, detailed morphological characterizations and multi-locus phylogenetic analyses were performed. Based on these analyses, the isolates were grouped into four main clades: section Ulocladioides, section Infectoriae, including two new species, section Embellisioides, and section Eureka, including one new species. These isolates were also characterized for their virulence under green-house conditions. They were able to produce leaf spot symptoms on tomato plants but with variable levels.

show abstract

“…Keissler, A. tomatophila Simmons, A. solani Sorauer and A. tomato (Cooke) LR Jones (Blancard, 2012;Snowdon 1990). A. solani is a plant pathogen and causal agent of early blight in solanum species (Russell et al, 2010); however, there are reports of A. alternata causing early blight of tomato at Pakistan and China (Akhtar et al, 2004;Ren et al, 2017). Due the above mentioned the objective of the research was to identify the agent associated with early blight in tomato at Morelos.…”

Section: Introductionmentioning

confidence: 99%

AGENT ASSOCIATED WITH EARLY BLIGHT Alternaria alternata (FR.) KEISSLER IN THE Solanum lycopersicum L. TOMATO CROP IN MEXICO

Arizpe

Cepeda

Vázquez

et al. 2021

Trop Subtrop Agroecosyst

View full text Add to dashboard Cite

Background. Tomato is an important crop in Mexico, due mainly to its economic profitability. Objective. The objective of the research was to identify the agent associated with early blight in tomato at Morelos. Methodology. Samples of diseased plants were taken from a greenhouse at Jantetelco country of the Morelos state and taken to the laboratory. Later, 1 cm cuts of diseased and healthy tissue were made which disinfected with 3% sodium hypochlorite for 1 min and washed three times with sterile distilled water for 1 min. Later, four leaf cuts in were put in Petri dishes with PDA culture medium (Bioxon) kept at 25 °C ± 2 °C for 168 h, the pathogen purification was done by monoconidial cultures and pathogen identifying by morphocultural criteria and molecularly. Results. The agent associated with early blight was Alternaria alternata. Implications. Our result reveals the opportunity to improve the control of this pathogen. Conclusion. Although A. alternata to infect the fruit peduncle causing abortion, in this research the pathogen was found also in leaves.

show abstract

First Report of Alternaria alternata Causing Black Spot on Tomato (Solanum lycopersicum) in Tongzhou, China

Cited by 4 publications

References 5 publications

Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats

Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats

Characterization of New Small-Spored Alternaria Species Isolated from Solanaceae in Algeria

AGENT ASSOCIATED WITH EARLY BLIGHT Alternaria alternata (FR.) KEISSLER IN THE Solanum lycopersicum L. TOMATO CROP IN MEXICO

Contact Info

Product

Resources

About