First Report of Bean pod mottle virus in Soybean in Canada

Abstract

In 2001, soybean fields were surveyed to determine the incidence of viruses because soybean aphids (Aphis glycines Matsamura), known to transmit Soybean mosaic virus (SMV) (2), were found in Ontario. In addition, bean leaf beetle (Cerotoma trifurcata Forster) was found during 2000 to be contaminated with Bean pod mottle virus (BPMV), although soybean plants, on which the beetles were feeding, tested negative (3). In the current survey, young soybean leaves were selected at random in July and August from 20 plants per site at growth stages R4 to R5 (1) from 415 sites representing the entire soybean-producing area in Ontario. Samples were maintained under cool conditions until received at the laboratory, where they were promptly processed. A combined sub-sample was obtained from the 20 plants per site. The 415 sub-samples were tested for SMV, BPMV, Tobacco ringspot virus (TRSV), and Tobacco streak virus (TSV) using polyclonal antibody kits for double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (Agdia Inc., Elkart, IN). The ELISA plates were read with a plate reader (MRX, Dynex Technologies Inc., Chantilly, VA), and results were analyzed using ELISA software (Leading Edge Research, Merrickville, Ontario) and compared positive and negative controls (Agdia). TRSV was detected in one sample from Essex County and another sample from Middlesex County. SMV, BPMV, and TSV were not found in commercial soybean fields. However, SMV and BPMV were found in samples originating from two soybean breeding nurseries, one in Essex County and one in Kent County. Seedlings of soybean cv. Williams 82 were inoculated in the greenhouse with sap from leaf samples that tested positive for BPMV. Leaves of plants that developed mosaic symptoms were retested using ELISA and confirmed to be positive for BPMV. SMV and TRSV have been found previously in commercial soybean fields in Ontario (4). To our knowledge, this is the first report of BPMV on soybean plants in Canada. References: (1) W. R. Fehr et al. Merr. Crop. Sci. 11:929, 1971. (2) J. H. Hill et al. Plant Dis. 85:561, 2001. (3) A. U. Tenuta. Crop Pest. 5 (11):8, 2000. (4) J. C. Tu. Can. J. Plant Sci. 66:491, 1986.

Bean pod mottle virus: A Threat to U.S. Soybean Production

“…BPMV was confirmed in North Carolina and Virginia (82), Kentucky (27), Mississippi (62), and Louisiana (35 (44) and has also been reported in Canada (50). BPMV is likely present in all soybean-producing states, but documentation is incomplete.…”

Microsatellite diversity of soybean genotypes differing in bean pod mottle virus leaf symptom

“…Bean pod mottle virus was first reported in soybean in Arkansas (Skotland 1958). Currently it is present in most soybean growing states of the United States of America (Horn et al 1970;Pitre et al 1979;Hopkins and Mueller 1983;Lin and Hill 1983;Zettler et al 1989;Giesler et al 2002) and also in Canada (Michelutti et al 2002). The first report of BPMV in Ohio was published in 1999 (Dorrance et al 2001).…”

A Cocktail ELISA Semi‐nested RT‐PCR Assay to Detect Bean pod mottle virus in Soya bean Seeds

“…BPMV infection of Phaseolus vulgaris (a bean of the Tendergreen variety) was first reported by Thomas and Zaumeyer in the United States (Zaumeyer and Thomas 1948) and has subsequently been reported in more than 10 countries including Brazil, Canada, Iran and Peru (Fribourg and Perez 1994;Anjos et al 1999;Giesler et al 2002;Michelutti et al 2002). Severe BPMV infections commonly occur in the United States, and almost all of the isolates and strains of BPMV reported in the GenBank database are derived from plants in the United States.…”