First Report of <i>Cotton leaf curl Gezira virus</i> and Its Associated Alphasatellite and Betasatellite from Disease Affected Okra Plants in the United States

Villegas, Cecilia; Ramos-Sobrinho, Roberto; Jifon, John; Keith, Cory Von; Rwahnih, Maher Al; Sétamou, Mamoudou; Brown, Judith K.; Alabi, Olufemi J.

doi:10.1094/pdis-06-19-1175-pdn

Cited by 9 publications

(9 citation statements)

References 11 publications

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“…CLCuGV-satellite complex members have lately been found in the African Sahel, Arabian Peninsula and the Middle East, Pakistan, and recently in okra in southern Texas, USA ( Tiendrébéogo et al, 2010 ; Tahir et al, 2011 ; Idris et al, 2014 ; Villegas et al, 2019 ). The origin of this OW CLCuGV-satellite complex in NW okra plants is unknown; however, it was possibly introduced by diseased plant materials or virulent whiteflies brought into Southern Texas, which is known for its extensive cotton and vegetable production.…”

Section: Introductionmentioning

confidence: 99%

“…The origin of this OW CLCuGV-satellite complex in NW okra plants is unknown; however, it was possibly introduced by diseased plant materials or virulent whiteflies brought into Southern Texas, which is known for its extensive cotton and vegetable production. The spread of this disease poses a significant threat because cotton and vegetable cultivars grown in the US have no resistance to CLCuGV ( Villegas et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

Qureshi

Lal²,

Nawaz-ul-Rehman³

et al. 2022

Front. Plant Sci.

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Plant viruses are responsible for the most devastating and commercially significant plant diseases, especially in tropical and subtropical regions. The genus begomovirus is the largest one in the family Geminiviridae, with a single-stranded DNA genome, either monopartite or bipartite. Begomoviruses are transmitted by insect vectors, such as Bemisia tabaci. Begomoviruses are the major causative agents of diseases in agriculture globally. Because of their diversity and mode of evolution, they are thought to be geographic specific. The emerging begomoviruses are of serious concern due to their increasing host range and geographical expansion. Several begomoviruses of Asiatic origin have been reported in Europe, causing massive economic losses; insect-borne transmission of viruses is a critical factor in virus outbreaks in new geographical regions. This review highlights crucial information regarding Asia’s four emerging and highly destructive begomoviruses. We also provided information regarding several less common but still potentially important pathogens of different crops. This information will aid possible direction of future studies in adopting preventive measures to combat these emerging viruses.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

Qureshi

Lal²,

Nawaz-ul-Rehman³

et al. 2022

Front. Plant Sci.

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“…Interestingly, the CLCuGeV isolates from Saudi Arabia were separately clustered with the CLCuGeV isolates reported from Asia and more closely grouped with the CLCuGeV isolates from Pakistan and Iran. CLCuGeV is an African Malvaceae -adapted monopartite begomovirus, and now it has spread into the Indo–Pak subcontinent, the Middle East [ 19 , 34 , 35 ], and has recently been identified in the USA [ 36 ]. In Saudi Arabia, CLCuGeV has been reported earlier from okra [ 19 ]; however, the identification of CLCuGeV isolates from new crop hosts is alarming and may lead to widespread crop infection.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Alhudaib

Almaghasla

El-Ganainy

et al. 2022

Plants

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The studies on the prevalence and genetic diversity of begomoviruses in Saudi Arabia are minimal. In this study, field-grown symptomatic tomato and muskmelon plants were collected, and initially, begomovirus infection was confirmed by the core coat protein sequences. Four tomato and two muskmelon plants with viral infections were further evaluated for Illumina MiSeq sequencing, and twelve sequences (2.7–2.8 kb) equivalent to the full-length DNA-A or DNA-B components of begomoviruses were obtained along with eight sequences (~1.3–1.4 kb) equivalent to the begomovirus-associated DNA-satellite components. Four begomovirus sequences obtained from tomato plants were variants of tomato yellow leaf curl virus (TYLCV) with nt sequence identities of 95.3–100%. Additionally, two tomato plants showed a mixed infection of TYLCV and cotton leaf curl Gezira virus (CLCuGeV), okra yellow crinkle Cameroon alphasatellite (OYCrCMA), and okra leaf curl Oman betasatellite (OLCuOMB). Meanwhile, from muskmelon plants, two sequences were closely related (99–99.6%) to the tomato leaf curl Palampur virus (ToLCPalV) DNA-A, whereas two other sequences showed 97.9-100% sequence identities to DNA-B of ToLCPalV, respectively. Complete genome sequences of CLCuGeV and associated DNA-satellites were also obtained from these muskmelon plants. The nt sequence identities of the CLCuGeV, OYCrCMA, and OLCuOMB isolates obtained were 98.3–100%, 99.5–100%, and 95.6–99.7% with their respective available variants. The recombination was only detected in TYLCV and OLCuOMB isolates. To our knowledge, this is the first identification of a mixed infection of bipartite and monopartite begomoviruses associated with DNA-satellites from tomato and muskmelon in Saudi Arabia. The begomovirus variants reported in this study were clustered with Iranian isolates of respective begomovirus components in the phylogenetic dendrogram. Thus, the Iranian agroecological route can be a possible introduction of these begomoviruses and/or their associated DNA-satellites into Saudi Arabia.

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“…NW alphasatellites are phylogenetically distinct (classified under the genera Clecrusatellite ) and associated with bipartite viruses [ 11 , 12 ]. Recently, alphasatellites of African origin (CLCGezA) were also reported in the Americas [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Patterns of Genetic Diversity among Alphasatellites Infecting Gossypium Species

et al. 2022

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Alphasatellites are small single-stranded circular DNA molecules associated with geminiviruses and nanoviruses. In this study, a meta-analysis of known alphasatellites isolated from the genus Gossypium (cotton) over the last two decades was performed. The phylogenetic and pairwise sequence identity analysis suggested that cotton-infecting begomoviruses were associated with at least 12 different alphasatellites globally. Three out of twelve alphasatellite were associated with cotton leaf curl geminiviruses but were not isolated from cotton plants. The cotton leaf curl Multan alphasatellite, which was initially isolated from cotton, has now been reported in several plant species, including monocot plants such as sugarcane. Our recombination analysis suggested that four alphasatellites, namely cotton leaf curl Lucknow alphasatellites, cotton leaf curl Multan alphasatellites, Ageratum yellow vein Indian alphasatellites and Ageratum enation alphasatellites, evolved through recombination. Additionally, high genetic variability was detected among the cotton-infecting alphasatellites at the genome level. The nucleotide substitution rate for the replication protein of alphasatellites (alpha-Rep) was estimated to be relatively high (~1.56 × 10−3). However, unlike other begomoviruses and satellites, the first codon position of alpha-Rep rapidly changed compared to the second and third codon positions. This study highlights the biodiversity and recombination of alphasatellites associated with the leaf curl diseases of cotton crops.

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First Report of Cotton leaf curl Gezira virus and Its Associated Alphasatellite and Betasatellite from Disease Affected Okra Plants in the United States

Cited by 9 publications

References 11 publications

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Patterns of Genetic Diversity among Alphasatellites Infecting Gossypium Species

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