First Report of <i>Diaporthe gulyae</i> Causing Phomopsis Stem Canker of Sunflower (<i>Helianthus annuus</i>) in Argentina

Mancebo, M. F.; Bazzalo, M. E.; Reid, Roberto; Kontz, Brian; Mathew, Febina M.

doi:10.1094/pdis-10-18-1907-pdn

Cited by 4 publications

(6 citation statements)

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“…described by Mancebo et al (2019), Isol4 and Isol5 grouped with D. helianthi and D. gulyae, respectively (bootstrap 100% for both isolates; Fig. 4).…”

Section: Figurementioning

confidence: 94%

“…DNA was extracted from one isolate selected as morphologically typical of each of the four species of Diaporthe reported in the present work, and they were identified as isolates 1, 2, 3, and 6 (Isol1, Isol2, Isol3, and Isol6, respectively). In the analysis were also included isolates 4 (Isol4) and 5 (Isol5), corresponding to D. helianthi and D. gulyae, respectively, previously described in Mancebo et al (2019). Mycelial discs of the four isolates were cut from the margin of actively growing colonies and transferred to 150 ml of potato dextrose liquid medium.…”

Section: Dna Isolation Pcr Amplification and Dna Sequencingmentioning

confidence: 99%

“…The PCR fragment sizes obtained were approximately 580 bp for ITS, 350 bp for EF1-a, and 530 bp for b-tubulin. The ITS and EF1-a from Isol4 and Isol5 were taken from deposited sequences in GenBank, whereas the b-tubulin gene region was amplified from genomic DNA isolated and stored by Mancebo et al (2019). The DNA samples were sequenced (Functional Biosciences, Madison, WI) using the same primer pairs.…”

Section: Longicollamentioning

confidence: 99%

“…The pathogenicity of Isol1, Isol2, Isol3, and Isol6 was evaluated by inoculating plants of a susceptible experimental hybrid (Advanta H1) at the vegetative phenological stage V4 (four leaves) to V6 (six leaves) of crop development (Schneiter and Miller 1981). Isolates of D. helianthi and D. gulyae sampled by Mancebo et al (2019) were included as positive controls.…”

Section: Pathogenicity Of Isolates Of Diaporthementioning

confidence: 99%

“…However, fungicide applications may not be effective after the PSC symptoms appear on the sunflower plants. Recent research characterizing species of Diaporthe causing PSC using morphology as well as analyses of translation elongation factor 1-a (EF1-a) and internal transcribed spacer (ITS) gene regions confirmed the presence of D. helianthi and, for the first time in Argentina, of D. gulyae (Mancebo et al 2019).…”

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confidence: 99%

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Six Species of Diaporthe Associated with Phomopsis Stem Canker of Sunflower in Southern Pampean Region of Argentina

Zambelli

Mancebo²,

Bazzalo³

et al. 2021

Plant Health Progress

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A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of β-tubulin, translation elongation factor 1-α, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.

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“…described by Mancebo et al (2019), Isol4 and Isol5 grouped with D. helianthi and D. gulyae, respectively (bootstrap 100% for both isolates; Fig. 4).…”

Section: Figurementioning

confidence: 94%

Section: Dna Isolation Pcr Amplification and Dna Sequencingmentioning

confidence: 99%

Section: Longicollamentioning

confidence: 99%

Section: Pathogenicity Of Isolates Of Diaporthementioning

confidence: 99%

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confidence: 99%

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