First Report of <i>Fusarium incarnatum</i> Associated With Fruit Disease of Bell Peppers in Trinidad

Ramdial, Hema; Hosein, Fazeeda N.; Rampersad, Sephra N.

doi:10.1094/pdis-05-15-0550-pdn

Cited by 31 publications

(17 citation statements)

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“…Furthermore, the pathogenicity test revealed that inoculation of this pathogen into muskmelon was successful only when muskmelons were wounded (Figure ), indicating that wounding may help fungal pathogens penetrate into plant tissues to colonize and cause diseases in plant hosts. This is in agreement with previous reports that wounding is required for successful (positive) pathogenicity tests with F. incarnatum (Ramdial, Hosein, & Rampersad, ). Overall, the results suggest that F. incarnatum causes severe fruit rot in muskmelon.…”

Section: Discussionsupporting

confidence: 93%

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Wonglom

Sunpapao

2020

Journal of Phytopathology

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Postharvest fruit rot was observed on muskmelon (Cucumis melo) on market shelves at a melon farm in the city of Hatyai, Songkhla, Thailand. Diseased muskmelon fruit displayed cotton‐like mycelia on wounds and had brown to dark brown internal tissue. Based on morphological characteristics and molecular analysis of internal transcribed spacer (ITS) and translation elongation factor 1‐α (TEF1‐α) DNA sequences, the fungal pathogen was identified as Fusarium incarnatum. This species belongs to the Fusarium incarnatum‐equiseti species complex (FIESC). A pathogenicity test was conducted to verify Koch's postulates, and F. incarnatum was observed to cause fruit rot on muskmelon; symptoms of the disease were similar to those seen in the field. However, only artificially wounded melons became infected, suggesting that F. incarnatum is an obligate wound‐infecting pathogen. To our knowledge, this is the first report of F. incarnatum causing fruit rot of muskmelon in Thailand.

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Section: Discussionsupporting

confidence: 93%

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Wonglom

Sunpapao

2020

Journal of Phytopathology

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“…F . incarnatum can infect other crops, such as Capsicum annum L. [37] and Ziziphus jujube Mill. [38].…”

Section: Discussionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, ‘INTA Rojo’. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar ‘INTA Rojo’ and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology, ecology, and control of the pathogenic fungi of common beans in the field.

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“…However, the size of macroconidia differed, ranging from 12.5 to 17.5 μm in width and from 60 to 82.5 μm in length. Ramdial et al (2016) observed macroconidia in Fusarium incarnatum with four to five septa, measuring between 28 and 31 μm in length and 3 to 5 μm in width and presence of microconidia. In Fusarium equiseti, Lazreg et al (2014) observed the presence of macroconidia with five to six septa, length ranging from 31 to 45 μm and basal cell in foot format, and no microconidia were observed, as Lazarotto et al (2014) verified macroconidia mostly with 5 septa and absence of chlamydospore.…”

Section: /6mentioning

confidence: 89%

“…Ramdial et al (2016) observed for Fusarium incarnatum, white and dense aerial mycelium, and staining of the lower part of the plate ranging from cream to salmon.…”

Section: Morphophysiological Characterizationmentioning

confidence: 94%

Morphological, physiological and molecular characterization of Fusarium lacertarum causal agent of damping-off in Casuarina equisetifolia

Favaretto

Muniz

Poletto

et al. 2018

Agraria

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Casuarina (Casuarina equisetifolia L.) is a species used like wind break, in streets ornamentation, soils conservation and dunes fixation. The main form of propagation of this species is by seeds, although, in nursery, the fungus Fusarium lacertarum have been cause damping-off in seedlings. Thus, the objective of this work was to characterize morphologically, physiologically and molecularly the pathogen. To molecular characterization, pure colonies were cultivated in PDA and LCA culture media, and for physiological characterization, colonies were cultivated in PDA, SNA, Mathur and V8 culture media. The genomic segment most appropriated to identify this species was the tef1-α. The causal agent of damping-off in Casuarina equisetifolia seedlings was confirmed like F. lacertarum, and the most efficient genomic region to identification is tef1-α. The best incubation condition to conidia production of F. lacertarum was in Mathur medium and the largest daily average growth was in SNA medium. The morphological characteristics are equivalent to the characterization of F. incarnatum-equiseti species complex isolated. Key words: ITS; nursery; phytopathogens; tef1-αCaracterização morfofisiológica e molecular de Fusarium lacertarum agente causal de tombamento em Casuarina equisetifolia RESUMO: A casuarina (Casuarina equisetifolia L.) é uma espécie utilizada como quebra-ventos, na ornamentação de ruas, conservação de solos e fixação de dunas. Sua principal forma de propagação é seminal, entretanto, em viveiros, o fungo Fusarium lacertarum tem causado tombamento em plântulas. Dessa forma, o objetivo deste trabalho foi caracterizar morfofisiológica e molecularmente o patógeno. Para a caracterização molecular, colônias puras foram cultivadas nos meios de cultura BDA e FCA, e para caracterização fisiológica, colônias foram cultivadas nos meios BDA, SNA, Mathur e V8. O segmento genômico mais apropriado para identificação da espécie foi o tef1-α. O agente causal de tomabamento em plântulas de Casuarina equisetifolia foi confirmando como F. lacertarum, sendo a região genômica mais eficiente para identificação a tef1-α. A melhor condição de incubação visando à produção de conídios de F. lacertarum foi em meio Mathur e o maior crescimento médio diário foi em meio SNA. As características morfológicas condizem com a caracterização de isolados do complexo de espécies F. incarnatumequiseti. Palavras-chave: ITS; viveiro; fitopatógeno; tef1-αMorphological, physiological and molecular characterization of Fusarium lacertarum causal agent of damping-off in Casuarina equisetifolia Rev. Bras.

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First Report of Fusarium incarnatum Associated With Fruit Disease of Bell Peppers in Trinidad

Cited by 31 publications

References 2 publications

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Morphological, physiological and molecular characterization of Fusarium lacertarum causal agent of damping-off in Casuarina equisetifolia

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