First Report of <i>Hop stunt viroid</i> and <i>Citrus exocortis viroid</i> on Fig with Symptoms of Fig Mosaic Disease

Yakoubi, S.; Elleuch, Amine; Besaies, N.; Marrakchi, M.; Fakhfakh, H.

doi:10.1111/j.1439-0434.2007.01205.x

Cited by 24 publications

(9 citation statements)

References 17 publications

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“…None of the sampled trees showed symptoms, except for the four Lebanese samples which, however, were negative to HSVd in PCR and molecular hybridization tests. It is noteworthy that mulberry is the second species of the family Moraceae, the other being fig ( Ficus carica ) (Yakoubi et al. 2007), to be a host of HSVd.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy

Elbeaino

Kubaa

Choueiri

et al. 2011

Journal of Phytopathology

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The presence of Hop stunt viroid (HSVd) was detected using RT-PCR and Northern blot hybridization in five of 60 samples from symptomless mulberry trees (Morus alba) collected in Italian and Lebanese orchards in July 2010. Infection levels were c. 10% in Lebanese and 8% in Italian samples. Nucleotide alignments showed that sequences of the mulberry HSVd isolates shared 95-96% identity with those of the same viroid occurring elsewhere. In a phylogenetic tree, mulberry HSVd isolates clustered together with those of HSVd-citrus, regardless of their geographical origin. This is the first report of infection in mulberry trees by HSVd.

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Section: Resultsmentioning

confidence: 99%

Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy

Elbeaino

Kubaa

Choueiri

et al. 2011

Journal of Phytopathology

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“…Edible fig (Ficus carica L.) trees are known to be natural hosts of several viruses (Elbeaino et al, 2010(Elbeaino et al, , 2012Laney et al, 2012;Ale-Agha and Rakhshandehroo, 2014;Minafra et al, 2017). Viroids of the family Pospiviroidae, including hop stunt viroid, citrus exocortis viroid, and a viroid resembling apple dimple fruit viroid, have also been reported in fig trees (Yakoubi et al, 2007;Chiumenti et al, 2014). The latter viroid was initially identified in fig by high-throughput sequencing (HTS), a powerful technology that has been applied to investigate the virome of several plant species (Hadidi et al, 2016;Villamor et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Novel Fig-Associated Viroid-Like RNAs Containing Hammerhead Ribozymes in Both Polarity Strands Identified by High-Throughput Sequencing

Olmedo-Velarde

Navarro

et al. 2020

Front. Microbiol.

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Based on high-throughput sequencing (HTS) data, the existence of viroid-like RNAs (Vd-LRNAs) associated with fig trees grown in the Hawaiian Islands has been predicted. One of these RNAs has been characterized as a circular RNA ranging in size from 357 to 360 nucleotides. Structural and biochemical features of this RNA, tentatively named fig hammerhead viroid-like RNA (FHVd-LR), markedly resemble those previously reported for several viroids and viroid-like satellite RNAs (Vd-LsatRNAs), which are non-proteincoding RNAs infecting their hosts autonomously and in combination with a helper virus, respectively. The full-length sequence of FHVd-LR variants was determined by RT-PCR, cloning, and sequencing. Despite a low global sequence identity with known viroids and Vd-LsatRNAs, FHVd-LR contains a hammerhead ribozyme (HRz) in each polarity strand. Northern blot hybridization assays identified the circular and linear forms of both polarity strands of FHVd-LR and showed that one strand, assigned the (+) polarity, accumulates at higher levels than the (−) polarity strand in vivo. The (+) polarity RNA assumes a rodlike secondary structure of minimal free energy with the conserved domains of the HRzs located in opposition to each other, a feature typical of several viroids and Vd-LRNAs. The HRzs of both FHVd-LR polarity strands were shown to be active in vitro during transcription, self-cleaving the RNAs at the predicted sites. These data, together with the sequence variability observed in the cloned and sequenced full-length variants, indicate that FHVd-LR is a novel viroid or Vd-LsatRNA. According to HTS data, the coexistence of FHVd-LR of different sizes in the same host cannot be excluded. The relationships of FHVd-LR with previously reported viroids and Vd-LsatRNAs, and the need to perform bioassays to conclusively clarify the biological nature of this circular RNA, are discussed.

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“…It has been found in a wide range of hosts. HSVd infection appears to be latent in many hosts (Shikata 1990;Astruc et al 1996;Polivka et al 1996), whereas it is frequently pathogenic in others such as hop, cucumber, citrus, plum, peach, apricot, almond, jujube, mulberry and fig (Ragozzino et al 2002;Amari et al 2007;Yakoubi et al 2007;Fei et al 2009;Zhang et al 2009;Wang et al 2010;Elbeaino et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Variants of Hop stunt viroid Associated with Mulberry Vein Clearing in Iran

Mazhar

Bagherian

Izadpanah

2013

Journal of Phytopathology

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Samples of mulberry leaves showing vein clearing, yellow speckle and leaf deformation symptoms were collected in the Fars Province of Iran. Grafting of buds from affected trees onto a number of healthy mulberry rootstocks resulted in transmission of the pathogen 40 days postinoculation. Likewise, mechanical inoculation of sap from affected leaves resulted in induction of similar symptoms in mulberry seedlings. Total nucleic acid was extracted from leaf tissues and subjected to reverse transcription polymerase chain reaction (RT-PCR) using Hop stunt viroid (HSVd) and Citrus exocortis viroid (CEVd)-specific primers, followed by sequencing of PCR products. RT-PCR with HSVd primers amplified a 302-or 305-bp product from affected samples, but none from healthy plants. CEVd was not detected in affected trees. HSVd was also found in graft-and mechanically inoculated plants. Sequence analyses showed three variants of HSVd in symptomatic mulberries related to plum and peach variants of this viroid with 94.9% identity, but with only 93% identity to Lebanese and Italian mulberry isolates. This is the first report of HSVd associated with vein clearing in mulberry in Iran.

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First Report of Hop stunt viroid and Citrus exocortis viroid on Fig with Symptoms of Fig Mosaic Disease

Cited by 24 publications

References 17 publications

Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy

Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy

Novel Fig-Associated Viroid-Like RNAs Containing Hammerhead Ribozymes in Both Polarity Strands Identified by High-Throughput Sequencing

Variants of Hop stunt viroid Associated with Mulberry Vein Clearing in Iran

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