First Report of Iris yellow spot virus on Onion (Allium cepa) in Serbia

Bulajić, Aleksandra; Jović, Jelena; Krnjajić, Slobodan; Petrov, M.; Djekic, Ivana; Krstić, Branka

doi:10.1094/pdis-92-8-1247a

Cited by 10 publications

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“…Comparative evaluation of mechanical and thrips-transmission of IYSV indicated that only thrips-transmission was effective, and therefore was adopted further in the study. This is in line with the previous reports by Bulajić, 2008, Srinivasan et al (2010), and Naveed and Pappu (2012).…”

Section: Discussionsupporting

confidence: 94%

“…Virus transmission using T. tabaci was adopted for this study because mechanical inoculation attempted as described was not successful and this was also reported previously (Bulajić, 2008; Srinivasan et al, 2010; Naveed and Pappu, 2012). A cohort of 500 first instar (≤ 8 h-old) T. tabaci obtained from icipe ’s insectary were allowed to acquire virus by feeding on IYSV-infected Allium cepa var.…”

Section: Methodsmentioning

confidence: 79%

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Endophytic Colonization of Onions Induces Resistance Against Viruliferous Thrips and Virus Replication

et al. 2018

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In agricultural ecosystems, insect pests, pathogens, weather patterns, and reduced soil fertility pose major challenges to crop productivity and are responsible for significant yield losses worldwide. Iris yellow spot virus (IYSV) vectored by Thrips tabaci Lindeman, is a major hindrance to onion production in eastern Africa. Control measures often rely on insecticides with deleterious effects. Endophytes are one key alternative as they can play important roles in mediating induced systemic resistance. Hence, we examined the potential effect of endophytic fungus Hypocrea lixii (F3ST1) on feeding and replication of IYSV on endophyte-colonized (E+) and endophyte-free (E-) onion plants. For more precise assessment, replication was also tested using leaf disk bioassays and individual thrips. The number of feeding punctures was significantly lower in E+ as compared to E- plants. Disease level was significantly lower in E+ as compared to E- plants for four weeks post-exposure to thrips. IYSV replication was reduced by 2.5-fold in endophytic treatment on both whole plant and leaf disk assays. Thrips tabaci showed 2 times higher feeding activities on endophyte-free onion leaf disks as compared to the endophyte-inoculated leaf disks. Our results suggest potential utility of the endophytes to reduce feeding damage and virus infection on onion plants. Further studies should be conducted to elucidate the secondary metabolites involved in such endophyte-thrips-virus mediated interaction and determine whether the interactions extend for this and other onion varieties and viruses under field conditions.

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Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 79%

Endophytic Colonization of Onions Induces Resistance Against Viruliferous Thrips and Virus Replication

et al. 2018

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“…There is no information on damage and economic impact, except that estimated incidence on onion was high in Slovenia (29) and that severely infected plants eventually died in Spain (11). However, most recent records of IYSV outbreak in several European countries such as Italy (7), Germany (5), the UK (33), and Serbia (9), and the virus occurrence in different areas of France on onion and shallot (22), as well as a new report on leek in Spain (10) could represent increasing incidence of IYSV in Europe.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, survey for the virus has been initiated only by several countries, and in almost all of them IYSV has been detected only sporadically and in restricted areas. In addition to the Netherlands and Israel, IYSV has been recorded in Slovenia (29), Italy (7,13), Poland (8), Spain (10,11), France (22,23), Germany (5,28), the UK (33), and Serbia (9).…”

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confidence: 99%

Incidence and Distribution of Iris yellow spot virus on Onion in Serbia

et al. 2009

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In a survey to determine the presence and distribution of Iris yellow spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Bačka District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-like symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription–polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB from the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.

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“…IYSV is a recently emerging tospovirus, with outbreaks in onions recorded from Spain (Cordoba-Selles et al, 2005), Germany (Leinhos et al, 2007), Greece (Chatzivassiliou et al, 2009), Italy (Tomassoli et al, 2009), Serbia (Bulajic et al, 2008), the Netherlands (Hoedjes et al, 2011) and the UK (Mumford et al, 2008). T. tabaci is the only reported vector of IYSV (Cortes et al, 1998;Kritzman et al, 2001), but recently F. fusca has been described as a second vector in the USA (Srinavasan et al, 2012).…”

Section: Occurrence Of Tospoviruses In the Risk Assessment Areamentioning

confidence: 99%

Scientific Opinion on the pest categorisation of the tospoviruses

Rossi¹

2012

EFS2

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The European Commission requested EFSA’s Panel on Plant Health to perform the pest categorisation for the 24 viruses of the Tospovirus genus for the EU territory. The following tospoviruses were analysed: Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV), Iris yellow spot virus (IYSV), Polygonum ringspot virus (PolRSV), Groundnut ringspot virus (GRSV), Tomato chlorotic spot virus (TCSV), Alstroemeria necrotic streak virus (ANSV), Chrysanthemum stem necrosis virus (CSNV), Melon severe mosaic virus (MSMV), Tomato yellow (fruit) ring virus (TYRV), Tomato zonate spot virus (TZSV), Groundnut yellow spot virus (GYSV), Groundnut chlorotic fan-spot virus (GCFSV), Groundnut bud necrosis virus (GBNV), Zucchini lethal chlorosis virus (ZLCV), Capsicum chlorosis virus (CaCV), Watermelon bud necrosis virus (WBNV), Watermelon silver mottle virus (WSMoV), Tomato necrotic ringspot virus (TNRV), Calla lily chlorotic spot virus (CCSV), Melon yellow spot virus (MYSV), Soybean vein necrosis associated virus (SVNaV), Bean necrotic mosaic virus (BeNMV) and Pepper necrotic spot virus (PNSV). In reaching its conclusions, the Panel considered four parameters to be of critical importance in the risk assessment area: (i) the presence of a tospovirus, (ii) the existence of host plants, (iii) the existence of thrips vector species and (iv) the potential for damage to crops grown in Europe. Based on its analysis, the Panel concluded that the 24 viruses analysed could be allocated to four different risk groups. Seven viruses (GRSV, TCSV, ANSV, CSNV, MSMV, TYRV, TZSV) for which both thrips species vectors and natural or experimental hosts crops are present in the EU territory were considered by the Panel to represent the highest risk to the EU territory. In contrast, three viruses (INSV, IYSV and PolRSV) already present in the risk assessment area were not considered by the Panel to pose a risk justifying the development of full risk assessments.

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First Report of Iris yellow spot virus on Onion (Allium cepa) in Serbia

Cited by 10 publications

References 0 publications

Endophytic Colonization of Onions Induces Resistance Against Viruliferous Thrips and Virus Replication

Endophytic Colonization of Onions Induces Resistance Against Viruliferous Thrips and Virus Replication

Incidence and Distribution of Iris yellow spot virus on Onion in Serbia

Scientific Opinion on the pest categorisation of the tospoviruses

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