First Report of <i>Little cherry virus 1</i> in Chile

Fiore, Nicola; Fernández, Carolina; Quiroga, Nicolás; Pino, Ana María; Rivera, Lucia; Sagredo, K.; Zamorano, Alan

doi:10.1094/pdis-09-17-1407-pdn

Cited by 5 publications

(3 citation statements)

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“…All other sampled Prunus species were confirmed to be latently infected. Their etiology varied considerably between scion and rootstock varieties and also according to season, year of sampling and sometimes co-infection as observed in earlier reports [2,3,8,21,22,27,28,29,30,31,32,33,34,35,36,41].…”

Section: Discussionmentioning

confidence: 83%

“…The LChV-1 and -2 incidence and observed variability in all surveyed regions can mainly be attributed to the contribution of different plant material sources, their co-infectious status and their mode of propagation in long-established orchards. In a context of rapid and widespread disease worldwide, aspects like multiple infections in symptomless plant reservoirs are readily known to play a key epidemiological role [8,21,27,28,29,30,31,32,33,34,35]. Severe damage has occurred on sweet and sour cherry trees on which the LChD was observed, often leading, together with possible other biotic and abiotic factors, to full tree decline in devastated old orchards, as has been reported for most sensitive sweet cherry cultivars ([8], Supplementary Material).…”

Section: Discussionmentioning

confidence: 99%

“…Neither LChV-1 or LChV-2 are pollen- or seed-transmitted [2,3,4,8,9]. LChD was first reported in the USA and Canada in the late 1930s, occasionally in association with the Western X phytoplasma disease [8], and is now present on a broad range of stone fruit species in many other cherry-growing areas of the world [2,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24], including Belgium [25,26].…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput Sequencing Assists Studies in Genomic Variability and Epidemiology of Little Cherry Virus 1 and 2 infecting Prunus spp. in Belgium

Tahzima

Foucart²,

Peusens³

et al. 2019

Viruses

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Little cherry disease, caused by little cherry virus 1 (LChV-1) and little cherry virus 2 (LChV-2), which are both members of the family Closteroviridae, severely affects sweet (Prunus avium L.) and sour cherry (P. cerasus L.) orchards lifelong production worldwide. An intensive survey was conducted across different geographic regions of Belgium to study the disease presence on these perennial woody plants and related species. Symptomatic as well as non-symptomatic Prunus spp. trees tested positive via RT-PCR for LChV-1 and -2 in single or mixed infections, with a slightly higher incidence for LChV-1. Both viruses were widespread and highly prevalent in nearly all Prunus production areas as well as in private gardens and urban lane trees. The genetic diversity of Belgian LChV-1 and -2 isolates was assessed by Sanger sequencing of partial genomic regions. A total RNA High-Throughput Sequencing (HTS) approach confirmed the presence of both viruses, and revealed the occurrence of other Prunus-associated viruses, namely cherry virus A (CVA), prune dwarf virus (PDV) and prunus virus F (PrVF). The phylogenetic inference from full-length genomes revealed well-defined evolutionary phylogroups with high genetic variability and diversity for LChV-1 and LChV-2 Belgian isolates, yet with little or no correlation with planting area or cultivated varieties. The global diversity and the prevalence in horticultural areas of LChV-1 and -2 variants, in association with other recently described fruit tree viruses, are of particular concern. Future epidemiological implications as well as new investigation avenues are exhaustively discussed.

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