First Report of <i>Macrophomina phaseolina</i> Causing Charcoal Rot in Lima Bean (<i>Phaseolus lunatus</i>) in Brazil

Sousa, E.; Melo, Maruzanete Pereira de; Pires, Larissa Leandro; Silva, B. A.; García, Mynor Efraín Munguía; Mota, J. M.; Beserra, José Evando Aguiar

doi:10.1094/pdis-11-16-1661-pdn

Cited by 10 publications

(8 citation statements)

References 4 publications

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“…Many of these hosts are economically important crop plants including maize, soybean, canola, cotton, peanut, sunflower and sugar cane 5–10 . Mp is found throughout the world 11–15 , notably in warmer regions where crop diseases caused by the pathogen are typically associated with drought and heat stress 16 . Increasing numbers of first reports of Mp -caused crop diseases 6,9,12 combined with the possibility of global warming effects benefitting the spread of this pathogen through stressed hosts makes the need to effectively combat this pathogen imperative.…”

Section: Introductionmentioning

confidence: 99%

A novel Arabidopsis pathosystem reveals cooperation of multiple hormonal response-pathways in host resistance against the global crop destroyer Macrophomina phaseolina

Schroeder

Liu

Shirai

et al. 2019

Sci Rep

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Dubbed as a “global destroyer of crops”, the soil-borne fungus Macrophomina phaseolina (Mp) infects more than 500 plant species including many economically important cash crops. Host defenses against infection by this pathogen are poorly understood. We established interactions between Mp and Arabidopsis thaliana (Arabidopsis) as a model system to quantitatively assess host factors affecting the outcome of Mp infections. Using agar plate-based infection assays with different Arabidopsis genotypes, we found signaling mechanisms dependent on the plant hormones ethylene, jasmonic acid and salicylic acid to control host defense against this pathogen. By profiling host transcripts in Mp-infected roots of the wild-type Arabidopsis accession Col-0 and ein2/jar1, an ethylene/jasmonic acid-signaling deficient mutant that exhibits enhanced susceptibility to this pathogen, we identified hundreds of genes potentially contributing to a diverse array of defense responses, which seem coordinated by complex interplay between multiple hormonal response-pathways. Our results establish Mp/Arabidopsis interactions as a useful model pathosystem, allowing for application of the vast genomics-related resources of this versatile model plant to the systematic investigation of previously understudied host defenses against a major crop plant pathogen.

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Section: Introductionmentioning

confidence: 99%

A novel Arabidopsis pathosystem reveals cooperation of multiple hormonal response-pathways in host resistance against the global crop destroyer Macrophomina phaseolina

Schroeder

Liu

Shirai

et al. 2019

Sci Rep

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“…Both subphases were observed from the fungal isolates in the present study and to support their morphological identification, molecular analysis was carried out. Molecular identification of M. phaseolina in the present study was verified using ITS region and TEF1-a as adopted by Sousa et al [47]. Most of the previous studies relied on ITS to recognize M. phaseolina [48][49][50] and the others used species-specific primers [51][52][53].…”

Section: Discussionmentioning

confidence: 59%

Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia

et al. 2019

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Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-a) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-a grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts. ARTICLE HISTORY

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“…For example, GPDH sequences were used to identify Alternaria species [32], Curvularia inaequalis and Bipolaris spicifera [33]. TEF1 sequences were used to identify Macrophomina phaseolina [34] or used together with other genes [35]. Pre-sA_ten isolates did not cluster apart from PresA_alt isolates in phylogenetic trees based on ITS region, β-tubulin, ATPase or calmodulin genes.…”

Section: Plos Onementioning

confidence: 99%

Histone H3 gene is not a suitable marker to distinguish Alternaria tenuissima from A. alternata affecting potato

Zhang¹,

Tian²,

Duan³

et al. 2020

PLoS ONE

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Potato Alternaria leaf blight is one of the economically most important disease in potato production worldwide. A recent study reported a quick method to distinguish main Alternaria pathogens A. tenuissima, A. alternata, and A. solani using partial histone H3 gene sequences. Using this method, our collection of 79 isolates from 8 provinces in China were presumably separated into A. tenussima and A. alternata. But in depth morphological and genetic analysis casted doubt on this identification. Culture morphologies of six presumed A. alternata isolates (PresA_alt) and six presumed A. tenuissima isolates (PresA_ten) were not significantly different. PresA_ten isolates also produced conidia in branched chains which supposed to be A. aternata. Phylogenetic analyses were conducted using internal transcribed spacer region (ITS) and five genes commonly used for species identification including glyceraldehyde-3-phosphate dehydrogenase (GPDH), translation elongation factor 1-alpha (TEF1), β-tubulin, plasma membrane ATPase (ATPase), and calmodulin genes. The results showed that GPDH and TEF1 sequences of PresA_alt and PresA_ten isolates were identical. The 12 isolates did not cluster by presumed species neither by individual or concatenated sequence comparisons. The phylogeny-trait association analysis confirmed that the two group isolates were undistinguishable by those molecular markers. Analysis of histone H3 gene sequences revealed variable intron sequences between PresA_ten and PresA_alt isolates, but the amino acid sequences were identical. Our results indicate that the previously published method to distinguish Alternaria species based on histone H3 gene sequence variation is inaccurate and that the prevalence of A. tenuissima isolates in China was likely overestimated.

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First Report of Macrophomina phaseolina Causing Charcoal Rot in Lima Bean (Phaseolus lunatus) in Brazil

Cited by 10 publications

References 4 publications

A novel Arabidopsis pathosystem reveals cooperation of multiple hormonal response-pathways in host resistance against the global crop destroyer Macrophomina phaseolina

A novel Arabidopsis pathosystem reveals cooperation of multiple hormonal response-pathways in host resistance against the global crop destroyer Macrophomina phaseolina

Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia

Histone H3 gene is not a suitable marker to distinguish Alternaria tenuissima from A. alternata affecting potato

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